GPX8 Polyclonal antibody

GPX8 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 16846-1-AP


GPx 8, GPX8, GSHPx 8, UNQ847

Tested Applications

Positive WB detected inU-251 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 16846-1-AP is GPX8 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPX8 fusion protein Ag9930
Full Name glutathione peroxidase 8 (putative)
Calculated Molecular Weight 209 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC029424
Gene Symbol GPX8
Gene ID (NCBI) 493869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPX8(Probable glutathione peroxidase 8) is also named as GSHPx-8 and belongs to the glutathione peroxidase family. Glutathione peroxidase (GPx) reduces hydroperoxides, including hydrogen peroxides, in the presence of reduced glutathione as a means of protecting organisms from oxidative damage. Several GPx isozymes have been identified in animal cells and these have been classified into different groups according to their cellular location and substrate specificity. In humans, eight types of GPx have been identified from GPx1 to GPx8. The full length GPX8 has 209 amino acids and the molecular weight is 24 kDa.


Product Specific Protocols
WB protocol for GPX8 antibody 16846-1-APDownload protocol
IHC protocol for GPX8 antibody 16846-1-APDownload protocol
IP protocol for GPX8 antibody 16846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Med Virol

SARS-CoV-2 nucleocapsid protein enhances the level of mitochondrial reactive oxygen species

Authors - Haiyun Yu

Free Radic Biol Med

ER-resident antioxidative GPx7 and GPx8 enzyme isoforms protect insulin-secreting INS-1E β-cells against lipotoxicity by improving the ER antioxidative capacity.

Authors - Ilir Mehmeti

Medicina (Kaunas)

Chosen Antioxidant Enzymes GPx4 and GPx8 in Human Colorectal Carcinoma: Study of the Slovak Population.

Authors - Andriana Pavliuk-Karachevtseva

MedComm (2020)

Glutathione peroxidase 8 expression on cancer cells and cancer-associated fibroblasts facilitates lung cancer metastasis

Authors - Yu‐Lian Xu
  • KD Validated


Luminal H(2) O(2) promotes ER Ca(2+) dysregulation and toxicity of palmitate in insulin-secreting INS-1E cells

Authors - Sarah Sharifi


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Andrea (Verified Customer) (01-23-2023)

Sharp and strong signal at 24 kDa with clear background.

  • Applications: Western Blot