GRAMD1A Polyclonal antibody

GRAMD1A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25487-1-AP

Synonyms

GRAM domain containing 1A, GRAMD1A, KIAA1533

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Validation Data Gallery

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  WB analysis using 25487-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25487-1-AP (GRAMD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293T cells, HeLa cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25487-1-AP targets GRAMD1A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRAMD1A fusion protein Ag22221
• Full Name: GRAM domain containing 1A
• Calculated Molecular Weight: 724 aa, 81 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC014077
• Gene Symbol: GRAMD1A
• Gene ID (NCBI): 57655
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein Aster-A (GRAMD1A) is a cholesterol transporter, which contains unique domains for binding cholesterol and the plasma membrane (PM), thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the endoplasmic reticulum (ER). It was reported that plays a role in autophagy regulation and is required for the biogenesis of the autophagosome (PMID:31222192). It can be detected as 80 kDa.