GSTA2 Polyclonal antibody

GSTA2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 10505-1-AP

Synonyms

Glutathione S transferase A2, GST class alpha member 2, GST gamma, GST HA subunit 2, GST2, GSTA2, GSTA2 2, GTA2, GTH2

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Validation Data Gallery

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  WB analysis of human testis using 10505-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10505-1-AP (GSTA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 10505-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10505-1-AP (GSTA2 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10505-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10505-1-AP (GSTA2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse brain using 10505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10505-1-AP (GSTA2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10505-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10505-1-AP (GSTA2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IHC detected in: human pancreas cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

10505-1-AP targets GSTA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSTA2 fusion protein Ag0781
• Full Name: glutathione S-transferase alpha 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC002895
• Gene Symbol: GSTA2
• Gene ID (NCBI): 2939
• RRID: AB_2248094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Theranostics; Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening.; Authors - Zhenyu Wang
rat	WB	Antioxidants (Basel); Isatin Counteracts Diethylnitrosamine/2-Acetylaminofluorene-Induced Hepatocarcinogenesis in Male Wistar Rats by Upregulating Anti-Inflammatory, Antioxidant, and Detoxification Pathways.; Authors - Nagwa G Tawfik
mouse	WB	Eur J Nutr; Multi-omics integration reveals the hepatoprotective mechanisms of ursolic acid intake against chronic alcohol consumption.; Authors - Xin Yan
human	WB	Kidney Blood Press Res; Identification of PDK4 as hub gene for diabetic nephropathy using co-expression network analysis; Authors - Yuanyuan Han
mouse	WB	J Pharmacol Sci; Enhanced expression of glutathione S-transferase in the hippocampus following acute treatment with trimethyltin in vivo.; Authors - Nagashima Reiko R
mouse	WB	J Toxicol Pathol; Thioacetamide-induced Hepatocellular Necrosis Is Attenuated in Diet-induced Obese Mice.; Authors - Shirai Makoto M

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iru (Verified Customer) (02-27-2019)

This antibody works best at 1:500 in TBST milk with O/N incubation. I get beautiful blot with the use of this antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Fallopian tube epithelial cells

GSTA2 Antibody Western Blot, validation (1:500 dilution) in Fallopian tube epithelial cells (Cat no:10505-1-AP)