GSTM3 Polyclonal antibody

• Featured Product
• KD/KO Validated

GSTM3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 15214-1-AP

Synonyms

Glutathione S transferase Mu 3, GST class mu 3, GST5, GSTB, GSTM3, GSTM3 3, GTM3, hGSTM3 3

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Validation Data Gallery

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  WB analysis using 15214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15214-1-AP (GSTM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15214-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15214-1-AP (GSTM3 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 15214-1-AP

  IP Result of anti-GSTM3 (IP:15214-1-AP, 4ug; Detection:15214-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 15214-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15214-1-AP (GSTM3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15214-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15214-1-AP (GSTM3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue, human testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 5 publications below

Product Information

15214-1-AP targets GSTM3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSTM3 fusion protein Ag7375
• Full Name: glutathione S-transferase mu 3 (brain)
• Calculated molecular weight: 27 kDa
• Observed molecular weight: 27-29 kDa
• GenBank accession number: BC000088
• Gene symbol: GSTM3
• Gene ID (NCBI): 2947
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSTM3 is a cytosolic enzyme involved in prostaglandin and leukotriene synthesis and in the metabolization of toxic compounds, such as chemotherapeutic drugs, insecticides, herbicides, carcinogens, and by-products of oxidative stress. GSTM3 is expressed in testis, brain, lung, and lymphocytes. GSTM3 have been reported to be dysregulated in cancers like prostate cancer and colon cancer.

Publications

Species	Application	Title
mouse	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
human	WB,IHC	Cancer Lett; GSTM3 deficiency impedes DNA mismatch repair to promote gastric tumorigenesis via CAND1/NRF2-KEAP1 signaling.; Authors - Tao Chen ; KD Validated
mouse	WB	Part Fibre Toxicol; Protein target identification and toxicological mechanism investigation of silver nanoparticles-induced hepatotoxicity by integrating proteomic and metallomic strategies.; Authors - Ming Xu
Sheep	IHC	Sci Rep; The fetal ovary exhibits temporal sensitivity to a 'real-life' mixture of environmental chemicals.; Authors - Richard G Lea
human	WB,IHC	Br J Cancer; Genetic variation in the GSTM3 promoter confer risk and prognosis of renal cell carcinoma by reducing gene expression.; Authors - Tan X X ; KD Validated
human	WB,IHC	Front Oncol; Glutathione S-Transferase Mu-3 Predicts a Better Prognosis and Inhibits Malignant Behavior and Glycolysis in Pancreatic Cancer.; Authors - Shunda Wang ; KD Validated