GSTM3 Polyclonal antibody

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  WB analysis using 15214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15214-1-AP (GSTM3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15214-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15214-1-AP (GSTM3 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 15214-1-AP

  IP result of anti-GSTM3 (IP:15214-1-AP, 4ug; Detection:15214-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of mouse testis using 15214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15214-1-AP (GSTM3 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15214-1-AP (GSTM3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 15214-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15214-1-AP (GSTM3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15214-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15214-1-AP (GSTM3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 15214-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSTM3 antibody (15214-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

GSTM3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15214-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

GST5, GST class-mu 3, GST class mu 3, Glutathione S-transferase Mu 3, Glutathione S transferase Mu 3

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Tested Applications

• Positive WB detected in: mouse testis tissue, human testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 16 publications below
• IHC: See 7 publications below

Product Information

15214-1-AP targets GSTM3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSTM3 fusion protein Ag7375
• Full Name: glutathione S-transferase mu 3 (brain)
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27-29 kDa
• GenBank Accession Number: BC000088
• Gene Symbol: GSTM3
• Gene ID (NCBI): 2947
• RRID: AB_2116070
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P21266
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSTM3 is a cytosolic enzyme involved in prostaglandin and leukotriene synthesis and in the metabolization of toxic compounds, such as chemotherapeutic drugs, insecticides, herbicides, carcinogens, and by-products of oxidative stress. GSTM3 is expressed in testis, brain, lung, and lymphocytes. GSTM3 have been reported to be dysregulated in cancers like prostate cancer and colon cancer.

Publications

Species	Application	Title
mouse	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
human	WB	Mol Cell; SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation; Authors - Wenxin Zhang
human	WB	Nat Chem Biol; Enhanced mapping of small-molecule binding sites in cells; Authors - Jacob M Wozniak
		Cell Syst; Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees.; Authors - Stephanie A Zlatic
human	WB,IHC	Cancer Lett; GSTM3 deficiency impedes DNA mismatch repair to promote gastric tumorigenesis via CAND1/NRF2-KEAP1 signaling.; Authors - Tao Chen ; KD Validated
mouse	WB	Part Fibre Toxicol; Protein target identification and toxicological mechanism investigation of silver nanoparticles-induced hepatotoxicity by integrating proteomic and metallomic strategies.; Authors - Ming Xu