GSTM3 Polyclonal antibody

• Featured Product
• KD/KO Validated

GSTM3 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 15214-1-AP

Synonyms

Glutathione S transferase Mu 3, GST class mu 3, GST5, GSTB, GSTM3, GSTM3 3, GTM3, hGSTM3 3

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Validation Data Gallery

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  WB analysis using 15214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15214-1-AP (GSTM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15214-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15214-1-AP (GSTM3 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 15214-1-AP

  IP Result of anti-GSTM3 (IP:15214-1-AP, 4ug; Detection:15214-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 15214-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15214-1-AP (GSTM3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15214-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15214-1-AP (GSTM3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue, human testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 5 publications below

Product Information

15214-1-AP targets GSTM3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSTM3 fusion protein Ag7375
• Full Name: glutathione S-transferase mu 3 (brain)
• Calculated molecular weight: 27 kDa
• Observed molecular weight: 27-29 kDa
• GenBank accession number: BC000088
• Gene symbol: GSTM3
• Gene ID (NCBI): 2947
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GSTM3 is a cytosolic enzyme involved in prostaglandin and leukotriene synthesis and in the metabolization of toxic compounds, such as chemotherapeutic drugs, insecticides, herbicides, carcinogens, and by-products of oxidative stress. GSTM3 is expressed in testis, brain, lung, and lymphocytes. GSTM3 have been reported to be dysregulated in cancers like prostate cancer and colon cancer.

Publications

Species	Application	Title
		Cell Syst; Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees.; Authors - Stephanie A Zlatic
human	WB	Cell Death Dis; Akt kinase LANCL2 functions as a key driver in EGFR-mutant lung adenocarcinoma tumorigenesis.; Authors - Yuqing Lou
human	WB	Int J Mol Sci; Glutathione S-Transferase M3 Is Associated with Glycolysis in Intrinsic Temozolomide-Resistant Glioblastoma Multiforme Cells.; Authors - Shu-Yu Cheng ; KD Validated
rat	WB	Exp Ther Med; iTRAQ-based quantitative proteomics analysis of the hepatoprotective effect of melatonin on ANIT-induced cholestasis in rats.; Authors - Dingnan Wang
human	WB	Endocrinology; Reassessment of the Proteomic Composition and Function of Extracellular Vesicles in the Seminal Plasma.; Authors - Hanshu Wang
sheep	IHC	Mol Cell Endocrinol; Exposure to chemical cocktails before or after conception - the effect of timing on ovarian development.; Authors - Bellingham Michelle M