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  • KD/KO Validated

GSTM3 Polyclonal antibody

GSTM3 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 15214-1-AP

Synonyms

Glutathione S transferase Mu 3, GST class mu 3, GST5, GSTB, GSTM3, GSTM3 3, GTM3, hGSTM3 3



Tested Applications

Positive WB detected inmouse testis tissue, human testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15214-1-AP targets GSTM3 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTM3 fusion protein Ag7375
Full Name glutathione S-transferase mu 3 (brain)
Calculated molecular weight 27 kDa
Observed molecular weight 27-29 kDa
GenBank accession numberBC000088
Gene symbol GSTM3
Gene ID (NCBI) 2947
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSTM3 is a cytosolic enzyme involved in prostaglandin and leukotriene synthesis and in the metabolization of toxic compounds, such as chemotherapeutic drugs, insecticides, herbicides, carcinogens, and by-products of oxidative stress. GSTM3 is expressed in testis, brain, lung, and lymphocytes. GSTM3 have been reported to be dysregulated in cancers like prostate cancer and colon cancer.

Protocols

Product Specific Protocols
WB protocol for GSTM3 antibody 15214-1-APDownload protocol
IHC protocol for GSTM3 antibody 15214-1-APDownload protocol
IP protocol for GSTM3 antibody 15214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Syst

Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees.

Authors - Stephanie A Zlatic
humanWB

Cell Death Dis

Akt kinase LANCL2 functions as a key driver in EGFR-mutant lung adenocarcinoma tumorigenesis.

Authors - Yuqing Lou
sheepIHC

Mol Cell Endocrinol

Exposure to chemical cocktails before or after conception - the effect of timing on ovarian development.

Authors - Bellingham Michelle M
humanWB,IHC

Front Oncol

Glutathione S-Transferase Mu-3 Predicts a Better Prognosis and Inhibits Malignant Behavior and Glycolysis in Pancreatic Cancer.

Authors - Shunda Wang
  • KD Validated
humanWB,IHC

Br J Cancer

Genetic variation in the GSTM3 promoter confer risk and prognosis of renal cell carcinoma by reducing gene expression.

Authors - Tan X X
  • KD Validated
SheepIHC

Sci Rep

The fetal ovary exhibits temporal sensitivity to a 'real-life' mixture of environmental chemicals.

Authors - Richard G Lea