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GSTM3 Polyclonal antibody

GSTM3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15214-1-AP

Synonyms

Glutathione S transferase Mu 3, GST class mu 3, GST5, GSTB, GSTM3, GSTM3 3, GTM3, hGSTM3 3



Tested Applications

Positive WB detected inmouse testis tissue, human testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

15214-1-AP targets GSTM3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, sheep, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTM3 fusion protein Ag7375
Full Name glutathione S-transferase mu 3 (brain)
Calculated molecular weight 27 kDa
Observed molecular weight 27-29 kDa
GenBank accession numberBC000088
Gene symbol GSTM3
Gene ID (NCBI) 2947
RRIDAB_2116070
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSTM3 is a cytosolic enzyme involved in prostaglandin and leukotriene synthesis and in the metabolization of toxic compounds, such as chemotherapeutic drugs, insecticides, herbicides, carcinogens, and by-products of oxidative stress. GSTM3 is expressed in testis, brain, lung, and lymphocytes. GSTM3 have been reported to be dysregulated in cancers like prostate cancer and colon cancer.

Protocols

Product Specific Protocols
WB protocol for GSTM3 antibody 15214-1-APDownload protocol
IHC protocol for GSTM3 antibody 15214-1-APDownload protocol
IP protocol for GSTM3 antibody 15214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
humanWB

Mol Cell

SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation

Authors - Wenxin Zhang

Cell Syst

Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees.

Authors - Stephanie A Zlatic
humanWB,IHC

Cancer Lett

GSTM3 deficiency impedes DNA mismatch repair to promote gastric tumorigenesis via CAND1/NRF2-KEAP1 signaling.

Authors - Tao Chen
  • KD Validated
mouseWB

Part Fibre Toxicol

Protein target identification and toxicological mechanism investigation of silver nanoparticles-induced hepatotoxicity by integrating proteomic and metallomic strategies.

Authors - Ming Xu
humanWB

Int J Mol Sci

Glutathione S-Transferase M3 Is Associated with Glycolysis in Intrinsic Temozolomide-Resistant Glioblastoma Multiforme Cells.

Authors - Shu-Yu Cheng
  • KD Validated