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GSTM3 Polyclonal antibody
GSTM3 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 15214-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse testis tissue, human testis tissue |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 4 publications below |
WB | See 9 publications below |
IHC | See 5 publications below |
Product Information
15214-1-AP targets GSTM3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, sheep |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GSTM3 fusion protein Ag7375 |
Full Name | glutathione S-transferase mu 3 (brain) |
Calculated molecular weight | 27 kDa |
Observed molecular weight | 27-29 kDa |
GenBank accession number | BC000088 |
Gene symbol | GSTM3 |
Gene ID (NCBI) | 2947 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GSTM3 is a cytosolic enzyme involved in prostaglandin and leukotriene synthesis and in the metabolization of toxic compounds, such as chemotherapeutic drugs, insecticides, herbicides, carcinogens, and by-products of oxidative stress. GSTM3 is expressed in testis, brain, lung, and lymphocytes. GSTM3 have been reported to be dysregulated in cancers like prostate cancer and colon cancer.
Protocols
Product Specific Protocols | |
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WB protocol for GSTM3 antibody 15214-1-AP | Download protocol |
IHC protocol for GSTM3 antibody 15214-1-AP | Download protocol |
IP protocol for GSTM3 antibody 15214-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Syst Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees. | ||
Cell Death Dis Akt kinase LANCL2 functions as a key driver in EGFR-mutant lung adenocarcinoma tumorigenesis. | ||
Int J Mol Sci Glutathione S-Transferase M3 Is Associated with Glycolysis in Intrinsic Temozolomide-Resistant Glioblastoma Multiforme Cells.
| ||
Exp Ther Med iTRAQ-based quantitative proteomics analysis of the hepatoprotective effect of melatonin on ANIT-induced cholestasis in rats. | ||
Endocrinology Reassessment of the Proteomic Composition and Function of Extracellular Vesicles in the Seminal Plasma. | ||
Mol Cell Endocrinol Exposure to chemical cocktails before or after conception - the effect of timing on ovarian development. |