Validation Data Gallery
|Positive WB detected in
|human brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue, HepG2 cells
|Positive IHC detected in
|human kidney tissue, human testis tissue, human skin tissue, human spleen tissue, human lung tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
15838-1-AP targets GSTT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, hamster
|Host / Isotype
|Rabbit / IgG
|GSTT1 fusion protein Ag8588
|glutathione S-transferase theta 1
|Calculated molecular weight
|240 aa, 27 kDa
|Observed molecular weight
|28 kDa, 30 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.
Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees.
Gstm1/Gstt1 is essential for reducing cisplatin ototoxicity in CBA/CaJ mice.
Determination of Hepatotoxicity and Its Underlying Metabolic Basis of 1,2-dichloropropane in Male Syrian Hamsters and B6C3F1 Mice.
Drug Metab Dispos
Evaluation of hepatic glutathione transferase Mu 1 and Theta 1 activities in humans and mice using genotype information.
Curr Issues Mol Biol
COPS5 Conferred the Platinum Resistance in Epithelial Ovarian Cancer
Int J Clin Exp Pathol
Different carcinogenic process in cholangiocarcinoma cases epidemically developing among workers of a printing company in Japan.