Tested Applications
| Positive WB detected in | human brain tissue, HepG2 cells, mouse brain tissue, mouse liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15838-1-AP targets GSTT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8588 Product name: Recombinant human GSTT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC007065 Sequence: MGLELYLDLLSQPCRAVYIFAKKNDIPFELRIVDLIKGQHLSDACAQVNPLKKVPALKDGDFTLTESVAILLYLTRKYKVPDYWYPQDLQARARVDEYLAWQHTTLRRSCLRALWHKVMFPVFLGEPVSPQTLAATLAELDVTLQLLEDKFLQNKAFLTGPHISLADLVAITELMHPVGAGCQVFEGRPKLATWRQRVEAAVGEDLFQEAHEVILKAKDFPPADPTIKQKLMPWVLAMIR Predict reactive species |
| Full Name | glutathione S-transferase theta 1 |
| Calculated Molecular Weight | 240 aa, 27 kDa |
| Observed Molecular Weight | 28 kDa, 30 kDa |
| GenBank Accession Number | BC007065 |
| Gene Symbol | GSTT1 |
| Gene ID (NCBI) | 2952 |
| RRID | AB_2116344 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30711 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GSTT1 antibody 15838-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GSTT1 antibody (Cat# 15838-1-AP) has 12 total citations across journals including Cell Reports, Cell Systems, Frontiers in Immunology, FASEB Journal, Toxicological Sciences, Drug Metabolism and Disposition, Current Issues in Molecular Biology, Journal of Psychiatric Research, Journal of Occupational Health, Journal of Toxicological Science, International Journal of Clinical and Experimental Pathology, and Journal of Animal Science and Biotechnology. Research fields span hepatotoxicity, ferroptosis, cisplatin resistance, cholangiocarcinoma, copper homeostasis, drug metabolism, and placental angiogenesis.
| Species | Application | Title |
|---|---|---|
J Anim Sci Biotechnol Increased maternal consumption of methionine as its hydroxyl analog improves placental angiogenesis and antioxidative capacity in sows | ||
Cell Rep The hepatic compensatory response to elevated systemic sulfide promotes diabetes. | ||
Cell Syst Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees. | ||
Front Immunol GSTT1/GSTM1 deficiency aggravated cisplatin-induced acute kidney injury via ROS-triggered ferroptosis | ||
Toxicol Sci Determination of Hepatotoxicity and Its Underlying Metabolic Basis of 1,2-dichloropropane in Male Syrian Hamsters and B6C3F1 Mice. | ||
FASEB J Gstm1/Gstt1 is essential for reducing cisplatin ototoxicity in CBA/CaJ mice.
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