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  • KD/KO Validated

GSTT1 Polyclonal antibody

GSTT1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15838-1-AP

Synonyms

Glutathione transferase T1 1, GST class theta 1, GSTT1



Tested Applications

Positive WB detected inhuman brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue, HepG2 cells
Positive IHC detected inhuman kidney tissue, human testis tissue, human skin tissue, human spleen tissue, human lung tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15838-1-AP targets GSTT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTT1 fusion protein Ag8588
Full Name glutathione S-transferase theta 1
Calculated Molecular Weight 240 aa, 27 kDa
Observed Molecular Weight 28 kDa, 30 kDa
GenBank Accession NumberBC007065
Gene Symbol GSTT1
Gene ID (NCBI) 2952
RRIDAB_2116344
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.

Protocols

Product Specific Protocols
WB protocol for GSTT1 antibody 15838-1-APDownload protocol
IHC protocol for GSTT1 antibody 15838-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Syst

Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees.

Authors - Stephanie A Zlatic
mouseWB

FASEB J

Gstm1/Gstt1 is essential for reducing cisplatin ototoxicity in CBA/CaJ mice.

Authors - Peipei Li
  • KO Validated
hamsterIHC

Toxicol Sci

Determination of Hepatotoxicity and Its Underlying Metabolic Basis of 1,2-dichloropropane in Male Syrian Hamsters and B6C3F1 Mice.

Authors - Min Wei
humanWB

Drug Metab Dispos

Evaluation of hepatic glutathione transferase Mu 1 and Theta 1 activities in humans and mice using genotype information.

Authors - Arakawa Shingo S
humanWB

Curr Issues Mol Biol

COPS5 Conferred the Platinum Resistance in Epithelial Ovarian Cancer

Authors - Ming-Wei Lin
mouseIHC

Int J Clin Exp Pathol

Different carcinogenic process in cholangiocarcinoma cases epidemically developing among workers of a printing company in Japan.

Authors - Yasunori Sato