Validation Data Gallery
|Positive WB detected in||human brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue, HepG2 cells|
|Positive IHC detected in||human kidney tissue, human testis tissue, human skin tissue, human spleen tissue, human lung tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15838-1-AP targets GSTT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, hamster|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GSTT1 fusion protein Ag8588|
|Full Name||glutathione S-transferase theta 1|
|Calculated molecular weight||240 aa, 27 kDa|
|Observed molecular weight||28 kDa, 30 kDa|
|GenBank accession number||BC007065|
|Gene ID (NCBI)||2952|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.
Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees.
The hepatic compensatory response to elevated systemic sulfide promotes diabetes.
J Occup Health
A trial to find appropriate animal models of dichloropropane-induced cholangiocarcinoma based on the hepatic distribution of glutathione S-transferases.
J Toxicol Sci
Modifying effects of 1,2-dichloropropane on N-nitrosobis(2-oxopropyl)amine-induced cholangiocarcinogenesis in male Syrian hamsters.
Int J Clin Exp Pathol
Different carcinogenic process in cholangiocarcinoma cases epidemically developing among workers of a printing company in Japan.
Drug Metab Dispos
Evaluation of hepatic glutathione transferase Mu 1 and Theta 1 activities in humans and mice using genotype information.