GenoMatrix™ Whole Genome Amplification Kit

Synonyms

genomatrix whole genome amplification, WGA, sample prep, sampleprep, sample preparation, genome, genome amplification, sheared DNA, unsheared DNA, DNA amplification

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Overview

Whole Genome Amplification (WGA) is used to amplify an entire genome from a small amount of genomic DNA, so any gene in the organism can be studied while preserving the limited sample. The GenoMatrix™ Whole Genome Amplification Kit provides up to a 500-fold amplification starting from as little as 10 ng of any type of genomic DNA, while maintaining sequence representation of the starting material. GenoMatrix Kits provide all reagents needed to quickly and easily perform whole genome amplification. The kit was designed for high-efficiency, genome-representative amplification, and is validated for use with samples generated by ChIP-IT^® Express and the MethylCollector™ Kit, which enables seamless transition into microarray analysis or other applications that require large quantities of DNA.

Description

What is the GenoMatrix Whole Genome Amplification Kit?

The GenoMatrix Whole Genome Amplification Kit provides all the reagents and buffers required to quickly and easily perform a genome-representative amplification of any type of genomic DNA. In particular, the method works especially well with ChIP samples and samples from MethylCollector™ Ultra experiments. All the reagents and enzymes provided in GenoMatrix have been optimized for the highest efficiency amplification. The GenoMatrix Kit includes sufficient reagents to perform 50 whole genome amplification reactions.

Preserve the sequence representation of your starting material

Active Motif’s GenoMatrix Whole Genome Amplification Kit utilizes a new approach that virtually eliminates amplification bias, so the amplified material has the same sequence representation as the material that you started with.

Quick and simple

The entire whole genome amplification procedure can be completed in less than two and half hours.

Versatile

The GenoMatrix Whole Genome Amplification Kit can be used as a stand-alone product to amplify unsheared DNA, or with pre-sheared samples generated through use of Active Motif’s ChIP-IT Express or MethylCollector Ultra Kits.

Contents

Contents & Storage

10X Shearing Buffer, GenoMatrix Primer A, GenoMatrix Buffer 1, GenoMatrix Buffer 2, GenoMatrix DNA polymerase, GenoMatrix Primer B, 10X PCR Buffer, Taq DNA polymerase, dNTPs and Human Genomic Control DNA. All reagents should be stored at -20°C. All reagents are guaranteed stable for 6 months when stored properly.