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Glutamine Synthetase Monoclonal antibody

Glutamine Synthetase Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b


human, pig and More (1)







Cat no : 66323-1-Ig


GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59

Tested Applications

Positive WB detected inJurkat cells, pig liver tissue, pig brain tissue
Positive IHC detected inhuman liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman brain tissue
Planning an IHC experiment? We recommend our IHCeasy GLUL Ready-To-Use IHC Kit. GLUL primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

66323-1-Ig targets Glutamine Synthetase in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Glutamine Synthetase fusion protein Ag6309
Full Name glutamate-ammonia ligase (glutamine synthetase)
Calculated molecular weight 374 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC011700
Gene symbol GLUL
Gene ID (NCBI) 2752
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).


Product Specific Protocols
WB protocol for Glutamine Synthetase antibody 66323-1-IgDownload protocol
IHC protocol for Glutamine Synthetase antibody 66323-1-IgDownload protocol
IF protocol for Glutamine Synthetase antibody 66323-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Acta Neuropathol

Selective vulnerability of tripartite synapses in amyotrophic lateral sclerosis.

Authors - Matthew J Broadhead

J Hepatol

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Authors - Weiqi Dai


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Calum (Verified Customer) (11-02-2021)

Glutamine synthetase (magenta) alongside alternative astrocyte marker GFAP (green) and DAPI (blue).

  • Applications: Immunofluorescence, Cell culture
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Primary Mouse Astrocytes
Glutamine Synthetase Antibody Immunofluorescence,Cell culture validation (1:500 dilution) in Primary Mouse Astrocytes (Cat no:66323-1-Ig)

Ryan (Verified Customer) (02-27-2019)

Tris-HCL antigen retrieval ph=9.

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish retina (Muller glia) at 3dpf
Glutamine Synthetase Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish retina (Muller glia) at 3dpf (Cat no:66323-1-Ig)