Glutamine Synthetase Monoclonal antibody

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• KD/KO Validated

Glutamine Synthetase Monoclonal Antibody for WB, IHC, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, zebrafish and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

CloneNo.

1D10G8

Reviews (2)

Cat no : 66323-1-Ig

Synonyms

GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59

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Validation Data Gallery

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  WB analysis of Jurkat using 66323-1-Ig

  WB result of Glutamine synthetase antibody (66323-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells.

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  WB analysis of Jurkat using 66323-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 66323-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66323-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66323-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66323-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66323-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66323-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66323-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 66323-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human brain using 66323-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human brain tissue using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of zebrafish retina using 66323-1-Ig

  Immunofluorescence analysis of the zebrafish retina at 5 days post fertilisation. Collagen18a1 (aka Endostatin) polyclonal antibody (18301-1-AP, magenta). Dilution 1:200. Glutamine synthetase (66323-1-Ig, green) dilution 1:500. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.

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Tested Applications

• Positive WB detected in: Jurkat cells, pig liver tissue, pig brain tissue
• Positive IHC detected in: human liver cancer tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: zebrafish retina, human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 4 publications below

Product Information

66323-1-Ig targets Glutamine Synthetase in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, zebrafish samples.

• Tested Reactivity: human, pig, zebrafish
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Glutamine Synthetase fusion protein Ag6309
• Full Name: glutamate-ammonia ligase (glutamine synthetase)
• Calculated Molecular Weight: 374 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC011700
• Gene Symbol: Glutamine Synthetase
• Gene ID (NCBI): 2752
• RRID: AB_2881703
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts (PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).

Publications

Species	Application	Title
		J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai
mouse	IF	Acta Neuropathol; Selective vulnerability of tripartite synapses in amyotrophic lateral sclerosis.; Authors - Matthew J Broadhead
		J Neurosci; C9ORF72 deficiency results in neurodegeneration in the zebrafish retina; Authors - Natalia Jaroszynska
zebrafish	IF	Life Sci Alliance; Unique activities of two overlapping PAX6 retinal enhancers; Authors - Kirsty Uttley
rat	IF	In Vitro Cell Dev Biol Anim; TRIM9 promotes Müller cell-derived retinal stem cells to differentiate into retinal ganglion cells by regulating Atoh7; Authors - Qi Zeng
mouse	IF	PLoS One; Excitatory to inhibitory synaptic ratios are unchanged at presymptomatic stages in multiple models of ALS; Authors - Calum Bonthron

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Calum (Verified Customer) (11-02-2021)

Glutamine synthetase (magenta) alongside alternative astrocyte marker GFAP (green) and DAPI (blue).

• Applications: Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Primary Mouse Astrocytes

Glutamine Synthetase Antibody Immunofluorescence,Cell culture validation (1:500 dilution) in Primary Mouse Astrocytes (Cat no:66323-1-Ig)

FH

Ryan (Verified Customer) (02-27-2019)

Tris-HCL antigen retrieval ph=9.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish retina (Muller glia) at 3dpf

Glutamine Synthetase Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish retina (Muller glia) at 3dpf (Cat no:66323-1-Ig)