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Glutamine Synthetase Monoclonal antibody

Glutamine Synthetase Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b


human, pig, zebrafish and More (2)







Cat no : 66323-1-Ig


GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59

Tested Applications

Positive WB detected inJurkat cells, pig liver tissue, pig brain tissue
Positive IHC detected inhuman liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inzebrafish retina, human brain tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66323-1-Ig targets Glutamine Synthetase in WB, IF, IHC, ELISA applications and shows reactivity with human, pig, zebrafish samples.

Tested Reactivity human, pig, zebrafish
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Glutamine Synthetase fusion protein Ag6309
Full Name glutamate-ammonia ligase (glutamine synthetase)
Calculated Molecular Weight 374 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC011700
Gene Symbol GLUL
Gene ID (NCBI) 2752
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts (PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).


Product Specific Protocols
WB protocol for Glutamine Synthetase antibody 66323-1-IgDownload protocol
IHC protocol for Glutamine Synthetase antibody 66323-1-IgDownload protocol
IF protocol for Glutamine Synthetase antibody 66323-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Hepatol

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Authors - Weiqi Dai

Acta Neuropathol

Selective vulnerability of tripartite synapses in amyotrophic lateral sclerosis.

Authors - Matthew J Broadhead

Life Sci Alliance

Unique activities of two overlapping PAX6 retinal enhancers

Authors - Kirsty Uttley

In Vitro Cell Dev Biol Anim

TRIM9 promotes Müller cell-derived retinal stem cells to differentiate into retinal ganglion cells by regulating Atoh7

Authors - Qi Zeng

Front Pharmacol

A novel method for generating glutamatergic SH-SY5Y neuron-like cells utilizing B-27 supplement

Authors - Emily-Rose Martin

Pharmacol Res

Therapeutic targeting of glutamate dehydrogenase 1 that links metabolic reprogramming and Snail-mediated epithelial-mesenchymal transition in drug-resistant lung cancer

Authors - Qizhi Wang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Calum (Verified Customer) (11-02-2021)

Glutamine synthetase (magenta) alongside alternative astrocyte marker GFAP (green) and DAPI (blue).

  • Applications: Immunofluorescence, Cell culture
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Primary Mouse Astrocytes
Glutamine Synthetase Antibody Immunofluorescence,Cell culture validation (1:500 dilution) in Primary Mouse Astrocytes (Cat no:66323-1-Ig)

Ryan (Verified Customer) (02-27-2019)

Tris-HCL antigen retrieval ph=9.

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish retina (Muller glia) at 3dpf
Glutamine Synthetase Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish retina (Muller glia) at 3dpf (Cat no:66323-1-Ig)