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Glutamine Synthetase Monoclonal antibody
Glutamine Synthetase Monoclonal Antibody for IF, IHC, WB,ELISA
Cat no : 66323-1-Ig
Validation Data Gallery
|Positive WB detected in||Jurkat cells, pig liver tissue, pig brain tissue|
|Positive IHC detected in||human liver cancer tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human brain tissue|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66323-1-Ig targets Glutamine Synthetase in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.
|Tested Reactivity||human, pig|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Glutamine Synthetase fusion protein Ag6309|
|Full Name||glutamate-ammonia ligase (glutamine synthetase)|
|Calculated molecular weight||374 aa, 42 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||BC011700|
|Gene ID (NCBI)||2752|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).
|Product Specific Protocols|
|WB protocol for Glutamine Synthetase antibody 66323-1-Ig||Download protocol|
|IHC protocol for Glutamine Synthetase antibody 66323-1-Ig||Download protocol|
|IF protocol for Glutamine Synthetase antibody 66323-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Calum (Verified Customer) (11-02-2021)
Glutamine synthetase (magenta) alongside alternative astrocyte marker GFAP (green) and DAPI (blue).
Ryan (Verified Customer) (02-27-2019)
Tris-HCL antigen retrieval ph=9.