Phospho-GSK3B (Ser389) Polyclonal antibody

Phospho-GSK3B (Ser389) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14850-1-AP

Synonyms

7330414F15Rik, 8430431H08Rik, C86142, Gsk3b, Ser389



Tested Applications

Positive WB detected inCalyculin A treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 36 publications below

Product Information

14850-1-AP targets Phospho-GSK3B (Ser389) in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name glycogen synthase kinase 3 beta
Calculated molecular weight 48 kDa
Observed molecular weight 46-50 kDa
GenBank accession numberBC060743
Gene symbol Gsk3b
Gene ID (NCBI) 56637
RRIDAB_2878085
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycogen synthase kinase 3s are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3α and GSK3β, encoded by separate genes, which are overall 85% homologous to each other, with 95% identity in the kinase domains. To date, phosphorylation at Ser9 by Akt is the best-characterized mechanism for the inhibition of GSK3β activity. GSK3β is phosphorylated at S389 in vivo by p38 MAPK and that this alternative regulatory mechanism of GSK3β is tissue-specific(PMID:18451303).

Protocols

Product Specific Protocols
WB protocol for Phospho-GSK3B (Ser389) antibody 14850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

Distinct dual roles of p-Tyr42 RhoA GTPase in tau phosphorylation and ATP citrate lyase activation upon different Aβ concentrations.

Authors - Kim Cuong Cap
humanWB

Cancer Res

p38 Stabilizes Snail by Suppressing DYRK2-Mediated Phosphorylation that is Required for GSK3β-βTrCP-Induced Snail Degradation.

Authors - Ki-Jun Ryu
ratWB

Food Funct

l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways

Authors - Ling Lin
humanWB

Int J Mol Sci

GSK3β Serine 389 Phosphorylation Modulates Cardiomyocyte Hypertrophy and Ischemic Injury.

Authors - Laura Vainio
humanWB

Front Oncol

FAM83A Promotes Lung Cancer Progression by Regulating the Wnt and Hippo Signaling Pathways and Indicates Poor Prognosis.

Authors - Yi-Wen Zheng
humanWB

Sci Rep

TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.

Authors - Fengqin Dong