|Positive WB detected in||Calyculin A treated NIH/3T3 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
14850-1-AP targets Phospho-GSK3B (Ser389) in WB, ELISA applications and shows reactivity with mouse, rat, human samples.
|Tested Reactivity||mouse, rat, human|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||glycogen synthase kinase 3 beta|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||46-50 kDa|
|GenBank accession number||BC060743|
|Gene ID (NCBI)||56637|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Glycogen synthase kinase 3s are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3α and GSK3β, encoded by separate genes, which are overall 85% homologous to each other, with 95% identity in the kinase domains. To date, phosphorylation at Ser9 by Akt is the best-characterized mechanism for the inhibition of GSK3β activity. GSK3β is phosphorylated at S389 in vivo by p38 MAPK and that this alternative regulatory mechanism of GSK3β is tissue-specific(PMID:18451303).
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GSK3β Serine 389 Phosphorylation Modulates Cardiomyocyte Hypertrophy and Ischemic Injury.