Phospho-GSK3B (Ser389) Polyclonal antibody

Phospho-GSK3B (Ser389) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14850-1-AP

Synonyms

7330414F15Rik, 8430431H08Rik, C86142, Gsk3b, Ser389



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 20 publications below

Product Information

14850-1-AP targets Phospho-GSK3B (Ser389) in WB, ELISA applications and shows reactivity with mouse, rat, human samples.

Tested Reactivity mouse, rat, human
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name glycogen synthase kinase 3 beta
Calculated molecular weight 48 kDa
Observed molecular weight 46-50 kDa
GenBank accession numberBC060743
Gene symbol Gsk3b
Gene ID (NCBI) 56637
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycogen synthase kinase 3s are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3α and GSK3β, encoded by separate genes, which are overall 85% homologous to each other, with 95% identity in the kinase domains. To date, phosphorylation at Ser9 by Akt is the best-characterized mechanism for the inhibition of GSK3β activity. GSK3β is phosphorylated at S389 in vivo by p38 MAPK and that this alternative regulatory mechanism of GSK3β is tissue-specific(PMID:18451303).

Protocols

Product Specific Protocols
WB protocol for Phospho-GSK3B (Ser389) antibody 14850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

PMID: 30816192

Authors - Lin Zhu
ratWB

Brain Res

PMID: 31121159

Authors - Xu Wang
humanWB

Front Oncol

FAM83A Promotes Lung Cancer Progression by Regulating the Wnt and Hippo Signaling Pathways and Indicates Poor Prognosis.

Authors - Yi-Wen Zheng
mouseWB

J Steroid Biochem Mol Biol

High glucose impaired estrogen receptor alpha signaling via β-catenin in osteoblastic MC3T3-E1.

Authors - Rui Wang
humanWB

Acta Biochim Biophys Sin (Shanghai)

FGFBP1-mediated crosstalk between fibroblasts and pancreatic cancer cells via FGF22/FGFR2 promotes invasion and metastasis of pancreatic cancer.

Authors - Zheng Zhang
humanWB

Mol Med Rep

Downregulation of microRNA‑199a‑5p attenuates hypoxia/reoxygenation‑induced cytotoxicity in cardiomyocytes by targeting the HIF‑1α‑GSK3β‑mPTP axis.

Authors - Da-Wei Liu