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HADHA Polyclonal antibody

HADHA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10758-1-AP

Synonyms

78 kDa gastrin binding protein, ECHA, HADH, HADHA, LCHAD, MTPA, TP ALPHA



Tested Applications

Positive WB detected inHEK-293 cells, MDA-MB-453s cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue, HeLa cells, HepG2 cells, mouse heart tissue, MCF-7 cells, Jurkat cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman heart tissue, human ovary tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10758-1-AP targets HADHA in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HADHA fusion protein Ag1211
Full Name hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
Calculated molecular weight 83 kDa
Observed molecular weight 70-79 kDa
GenBank accession numberBC009235
Gene symbol HADHA
Gene ID (NCBI) 3030
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).

Protocols

Product Specific Protocols
WB protocol for HADHA antibody 10758-1-APDownload protocol
IHC protocol for HADHA antibody 10758-1-APDownload protocol
IF protocol for HADHA antibody 10758-1-APDownload protocol
IP protocol for HADHA antibody 10758-1-APDownload protocol
FC protocol for HADHA antibody 10758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Authors - Wei Wang
humanWB

Mol Med Rep

Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout.

Authors - Jingjing Zhang
humanWB

Physiol Genomics

Men supplemented with 17beta-estradiol have increased beta-oxidation capacity in skeletal muscle.

Authors - Maher Amy C AC
ratWB

J Agric Food Chem

Effect of High-Fat Diet on Hepatic Proteomics of Hamsters.

Authors - Chen-Chung Liao
mouseWB

Am J Physiol Heart Circ Physiol

Acetylation of mitochondrial proteins by GCN5L1 promotes enhanced fatty acid oxidation in the heart.

Authors - Dharendra Thapa
humanWB

PLoS One

Women have higher protein content of beta-oxidation enzymes in skeletal muscle than men.

Authors - Maher Amy C AC