Back

HADHA Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 10758-1-AP

Featured Product KD/KO validated

Be the first to review this product

-_-

Freight/Packing

Con: 30 μg/150 μl

Choose size:

Please visit your regions distributor:

Request quote for
bulk/custom formulations

Special offer: Add control/tag antibody for $99
Load more

Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

HADHA Antibody WB HeLa cells 10758-1-AP

WB result of HADHA antibody (10758-1-AP, 1:4000) with si-control and si-HADHA transfected HeLa cells.

HADHA Antibody IF HeLa cells 10758-1-AP

IF result of HADHA antibody (10758-1-AP, 1:50) with si-control and si-HADHA transfected HeLa cells.

HADHA Antibody WB HEK-293 cells 10758-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

HADHA Antibody IP HEK-293 cells 10758-1-AP

IP Result of anti-HADHA (IP:10758-1-AP, 4ug; Detection:10758-1-AP 1:200) with HEK-293 cells lysate 2500ug.

HADHA Antibody IHC human colon cancer tissue 10758-1-AP

Immunohistochemical analysis of paraffin-embedded human colon cancer using 10758-1-AP(HADHA antibody) at dilution of 1:100 (under 40x lens)

HADHA Antibody IF HeLa cells 10758-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 10758-1-AP(HADHA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

HADHA Antibody FC HepG2 cells 10758-1-AP

1X10^6 HepG2 cells were stained with 0.2ug HADHA antibody (10758-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
HEK-293 cells, MCF-7 cells, MDA-MB-453s cells, Jurkat cells, mouse liver tissue, HeLa cells, rat kidney tissue, HepG2 cells, mouse kidney tissue, mouse heart tissue, HeLa cells
Positive IP detected in:
HEK-293 cells
Positive IHC detected in:
human colon cancer tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells, HepG2 cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:1000-1:5000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
HADHA fusion protein Ag1211
Full name:
hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
Calculated molecular weight:
83 kDa
Observed molecular weight:
70-79 kDa
GenBank accession number:
BC009235
Gene ID (NCBI):
3030
Gene symbol
HADHA
Synonyms
78 kDa gastrin binding protein, ECHA, HADH, HADHA, LCHAD, MTPA, TP ALPHA

Background

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).


Back
to top