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HADHA Polyclonal antibody
HADHA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 10758-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, MDA-MB-453s cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue, HeLa cells, HepG2 cells, mouse heart tissue, MCF-7 cells, Jurkat cells, NIH/3T3 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human heart tissue, human ovary tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 13 publications below |
IHC | See 1 publications below |
Product Information
10758-1-AP targets HADHA in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HADHA fusion protein Ag1211 |
Full Name | hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit |
Calculated molecular weight | 83 kDa |
Observed molecular weight | 70-79 kDa |
GenBank accession number | BC009235 |
Gene symbol | HADHA |
Gene ID (NCBI) | 3030 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HADHA antibody 10758-1-AP | Download protocol |
IHC protocol for HADHA antibody 10758-1-AP | Download protocol |
IF protocol for HADHA antibody 10758-1-AP | Download protocol |
IP protocol for HADHA antibody 10758-1-AP | Download protocol |
FC protocol for HADHA antibody 10758-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oxid Med Cell Longev Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation. | ||
Mol Med Rep Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout. | ||
Physiol Genomics Men supplemented with 17beta-estradiol have increased beta-oxidation capacity in skeletal muscle. | ||
Am J Physiol Heart Circ Physiol Acetylation of mitochondrial proteins by GCN5L1 promotes enhanced fatty acid oxidation in the heart. | ||
PLoS One Women have higher protein content of beta-oxidation enzymes in skeletal muscle than men. |