HADHA Polyclonal antibody

• Featured Product
• KD/KO Validated

HADHA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 10758-1-AP

Synonyms

78 kDa gastrin binding protein, ECHA, HADH, HADHA, LCHAD, MTPA, TP ALPHA

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Validation Data Gallery

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  WB analysis of HeLa cells using 10758-1-AP

  WB result of HADHA antibody (10758-1-AP, 1:4000) with si-control and si-HADHA transfected HeLa cells.

• 

  WB analysis using 10758-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10758-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 10758-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10758-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10758-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10758-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 10758-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10758-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10758-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10758-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10758-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10758-1-AP

  IP Result of anti-HADHA (IP:10758-1-AP, 4ug; Detection:10758-1-AP 1:200) with HEK-293 cells lysate 2500ug.

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  IHC staining of human heart using 10758-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 10758-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 10758-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10758-1-AP (HADHA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 10758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 10758-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 10758-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa cells using 10758-1-AP

  IF result of HADHA antibody (10758-1-AP, 1:50) with si-control and si-HADHA transfected HeLa cells.

• 

  IF Staining of HeLa using 10758-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10758-1-AP (HADHA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10758-1-AP

  Immunofluorescent analysis of HepG2 cells, using HADHA antibody 10758-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 10758-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug HADHA antibody (10758-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HEK-293 cells, MDA-MB-453s cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue, HeLa cells, HepG2 cells, mouse heart tissue, MCF-7 cells, Jurkat cells, NIH/3T3 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human heart tissue, human ovary tissue, human colon cancer tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 18 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

10758-1-AP targets HADHA in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HADHA fusion protein Ag1211
• Full Name: hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
• Calculated molecular weight: 83 kDa
• Observed molecular weight: 70-79 kDa
• GenBank accession number: BC009235
• Gene symbol: HADHA
• Gene ID (NCBI): 3030
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).

Publications

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.; Authors - Wei Wang
rat	CoIP	JCI Insight; Rescue of myocardial energetic dysfunction in diabetes through the correction of mitochondrial hyperacetylation by honokiol.; Authors - Matthew Kerr
human	WB	Mol Med Rep; Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout.; Authors - Jingjing Zhang
human	WB	Physiol Genomics; Men supplemented with 17beta-estradiol have increased beta-oxidation capacity in skeletal muscle.; Authors - Maher Amy C AC
rat	WB	J Agric Food Chem; Effect of High-Fat Diet on Hepatic Proteomics of Hamsters.; Authors - Chen-Chung Liao
mouse	WB	Am J Physiol Heart Circ Physiol; Acetylation of mitochondrial proteins by GCN5L1 promotes enhanced fatty acid oxidation in the heart.; Authors - Dharendra Thapa