Validation Data Gallery
|Positive WB detected in||HEK-293 cells, MDA-MB-453s cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue, HeLa cells, HepG2 cells, mouse heart tissue, MCF-7 cells, Jurkat cells, NIH/3T3 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human heart tissue, human ovary tissue, human lung tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10758-1-AP targets HADHA in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HADHA fusion protein Ag1211|
|Full Name||hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit|
|Calculated molecular weight||83 kDa|
|Observed molecular weight||70-79 kDa|
|GenBank accession number||BC009235|
|Gene ID (NCBI)||3030|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).
|Product Specific Protocols|
|WB protocol for HADHA antibody 10758-1-AP||Download protocol|
|IHC protocol for HADHA antibody 10758-1-AP||Download protocol|
|IF protocol for HADHA antibody 10758-1-AP||Download protocol|
|IP protocol for HADHA antibody 10758-1-AP||Download protocol|
|FC protocol for HADHA antibody 10758-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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