Various lysates were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10758-1-AP(HADHA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10758-1-AP(HADHA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

WB result of HADHA antibody (10758-1-AP, 1:4000) with si-control and si-HADHA transfected HeLa cells.

HepG2 cells were subjected to SDS PAGE followed by western blot with 10758-1-AP(HADHA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 10758-1-AP(HADHA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

MCF7 cells were subjected to SDS PAGE followed by western blot with 10758-1-AP(HADHA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10758-1-AP(HADHA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

mouse heart tissue were subjected to SDS PAGE followed by western blot with 10758-1-AP(HADHA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10758-1-AP(HADHA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 10758-1-AP(HADHA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

rat kidney tissue were subjected to SDS PAGE followed by western blot with 10758-1-AP( HADHA Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10758-1-AP (HADHA antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human lung using 10758-1-AP(HADHA antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human lung using 10758-1-AP(HADHA antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical analysis of paraffin-embedded human ovary using 10758-1-AP(HADHA antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human ovary using 10758-1-AP(HADHA antibody) at dilution of 1:50 (under 40x lens)

IP Result of anti-HADHA (IP:10758-1-AP, 4ug; Detection:10758-1-AP 1:200) with HEK-293 cells lysate 2500ug.

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 10758-1-AP(HADHA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IF result of HADHA antibody (10758-1-AP, 1:50) with si-control and si-HADHA transfected HeLa cells.

Immunofluorescent analysis of HepG2 cells, using HADHA antibody 10758-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

1X10^6 HepG2 cells were stained with 0.2ug HADHA antibody (10758-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.