HADHB Polyclonal antibody
HADHB Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 29091-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, MCF-7 cells, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat liver tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 1 publications below |
Product Information
29091-1-AP targets HADHB in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HADHB fusion protein Ag30298 |
Full Name | hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit |
Calculated molecular weight | 51 kDa |
Observed molecular weight | 52 kDa |
GenBank accession number | BC017564 |
Gene symbol | HADHB |
Gene ID (NCBI) | 3032 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HADHB, also named as TP- beta, Acetyl-CoA acyltransferase and Beta-ketothiolase, is a mitochondrial trifunctional enzyme subunit beta. Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity. HADHB has 2 isoforms produced by alternative splicing with the MW of 49 kDa and 51 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for HADHB antibody 29091-1-AP | Download protocol |
IHC protocol for HADHB antibody 29091-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. | ||
Front Oncol Hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta gene as a tumour suppressor in stomach adenocarcinoma
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