|Positive WB detected in||PC-3 cells, Daudi cells, Raji cells, RAW 264.7 cells|
|Positive FC detected in||Raji cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
11872-1-AP targets TIM3 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TIM3 fusion protein Ag2459|
|Full Name||hepatitis A virus cellular receptor 2|
|Calculated molecular weight||301 aa, 33 kDa|
|Observed molecular weight||50-70 kDa, 33 kDa|
|GenBank accession number||BC020843|
|Gene ID (NCBI)||84868|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301)
Front Mol Neurosci
Galectin-9/Tim-3 pathway mediates dopaminergic neurodegeneration in MPTP-induced mouse model of Parkinson’s disease
Front Cell Infect Microbiol
The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection.
CNS Neurosci Ther
PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.
Int J Oncol
Role of downregulation of galectin-9 in the tumorigenesis of gastric cancer.
Iran J Immunol
The Immunomodulatory Effects and Mechanisms of Tim-3 Action in the Early Stage of Mice with Severe Acute Pancreatitis.
Immun Inflamm Dis
Galectin-9 and myeloid-derived suppressor cell as prognostic indicators for chronic lymphocytic leukemia
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Qing (Verified Customer) (06-10-2022)
It work nicely