TIM3 Polyclonal antibody

TIM3 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC

Conjugate

Unconjugated

Cat no : 11872-1-AP

Synonyms

CD366, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIM-3, TIMD 3, TIMD3



Tested Applications

Positive WB detected inPC-3 cells, Daudi cells, Raji cells, RAW 264.7 cells
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

11872-1-AP targets TIM3 in WB, FC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIM3 fusion protein Ag2459
Full Name hepatitis A virus cellular receptor 2
Calculated molecular weight 301 aa, 33 kDa
Observed molecular weight 50-70 kDa, 33 kDa
GenBank accession numberBC020843
Gene symbol HAVCR2
Gene ID (NCBI) 84868
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301)

Protocols

Product Specific Protocols
WB protocol for TIM3 antibody 11872-1-APDownload protocol
FC protocol for TIM3 antibody 11872-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Iran J Immunol

The Immunomodulatory Effects and Mechanisms of Tim-3 Action in the Early Stage of Mice with Severe Acute Pancreatitis.

Authors - Min Lin

Int J Oncol

Role of downregulation of galectin-9 in the tumorigenesis of gastric cancer.

Authors - Jiaxing Yang
humanWB

Front Cell Infect Microbiol

The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection.

Authors - Teng Li