HBG2 Polyclonal antibody

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  WB analysis using 31753-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31753-1-AP (HBG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 31753-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31753-1-AP (HBG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HBG2 Polyclonal Antibody for WB, ELISA

Cat No. 31753-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

hemoglobin, gamma G, Hemoglobin subunit gamma 2, Hemoglobin gamma G chain, Hemoglobin gamma 2 chain, Hb F Ggamma

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Tested Applications

• Positive WB detected in: K-562 cells, TF-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31753-1-AP targets HBG2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HBG2 fusion protein Ag36028
• Full Name: hemoglobin, gamma G
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 14~16 kDa
• GenBank Accession Number: BC029387
• Gene Symbol: HBG2
• Gene ID (NCBI): 3048
• RRID: AB_3670105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P69892
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gamma globin genes (HBG2, encoding Gγ-globin; HBG1 encoding Aγ-globin) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. Gamma chain production continues into adulthood in some beta-thalassemias and related conditions.