HCN2 Polyclonal antibody

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  WB analysis of mouse brain using 55245-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55245-1-AP (HCN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55245-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55245-1-AP (HCN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55245-1-AP

  IP result of anti-HCN2 (IP:55245-1-AP, 4ug; Detection:55245-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 55245-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55245-1-AP (HCN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 55245-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55245-1-AP (HCN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 55245-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55245-1-AP (HCN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 55245-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55245-1-AP (HCN2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse heart using 55245-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using HCN2 antibody (55245-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L).

HCN2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 55245-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, HAC 1, Brain cyclic nucleotide-gated channel 2, BCNG-2, BCNG2

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Tested Applications

• Positive WB detected in: mouse brain tissue, Jurkat cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human heart tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

55245-1-AP targets HCN2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 2
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 97-110 kDa
• GenBank Accession Number: NM_001194
• Gene Symbol: HCN2
• Gene ID (NCBI): 610
• RRID: AB_10951108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UL51
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HCN2, also named as BCNG2, belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). HCN2 produces a large instantaneous current. It is activated by cAMP. HCN2 is modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. This antibody is specific to HCN2.

Publications

Species	Application	Title
human	WB,IF	Nucleic Acids Res; Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts.; Authors - Donghong Zhang
rat	WB	Mol Pain; HCN2 contributes to oxaliplatin-induced neuropathic pain through activation of the CaMKII/CREB cascade in spinal neurons.; Authors - Xiaoyu Liu
rat	WB,IHC	Brain Res Bull; HCN2 contributes to oxaliplatin-induced neuropathic pain by inducing spinal long-term potentiation via activation of NMDA receptor-mediated CaMKII signaling.; Authors - Xiaoyu Liu
mouse	WB	Exp Ther Med; Effect of mHCN2 gene modification on chronotropic relevant receptors in BMSCs co-cultured with atrial myocytes.; Authors - Chandong Ding
mouse	WB	Cell Rep; Optogenetic Activation of Striatopallidal Neurons Reveals Altered HCN Gating in DYT1 Dystonia.; Authors - Giuseppe Sciamanna
mouse	WB	Neurosci Bull; Thalamocortical Circuit Controls Neuropathic Pain via Up-regulation of HCN2 in the Ventral Posterolateral Thalamus; Authors - Yi Yan ; KD Validated