HCN2 Polyclonal antibody

HCN2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 55245-1-AP

Synonyms

HAC 1, HCN2



Tested Applications

Positive WB detected inmouse brain tissue, Jurkat cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55245-1-AP targets HCN2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name hyperpolarization activated cyclic nucleotide-gated potassium channel 2
Calculated molecular weight 97 kDa
Observed molecular weight 97-110 kDa
GenBank accession numberNM_001194
Gene symbol HCN2
Gene ID (NCBI) 610
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

HCN2, also named as BCNG2, belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). HCN2 produces a large instantaneous current. It is activated by cAMP. HCN2 is modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. This antibody is specific to HCN2.

Protocols

Product Specific Protocols
WB protocol for HCN2 antibody 55245-1-APDownload protocol
IHC protocol for HCN2 antibody 55245-1-APDownload protocol
IP protocol for HCN2 antibody 55245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

Optogenetic Activation of Striatopallidal Neurons Reveals Altered HCN Gating in DYT1 Dystonia.

Authors - Giuseppe Sciamanna
mouseWB

Exp Ther Med

Effect of mHCN2 gene modification on chronotropic relevant receptors in BMSCs co-cultured with atrial myocytes.

Authors - Chandong Ding
ratWB,IHC

Brain Res Bull

HCN2 contributes to oxaliplatin-induced neuropathic pain by inducing spinal long-term potentiation via activation of NMDA receptor-mediated CaMKII signaling.

Authors - Xiaoyu Liu
ratWB

Mol Pain

HCN2 contributes to oxaliplatin-induced neuropathic pain through activation of the CaMKII/CREB cascade in spinal neurons.

Authors - Xiaoyu Liu
humanWB,IF

Nucleic Acids Res

Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts.

Authors - Donghong Zhang