HCN2 Polyclonal antibody

HCN2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 55245-1-AP

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, HAC 1, Brain cyclic nucleotide-gated channel 2, BCNG-2, BCNG2



Tested Applications

Positive WB detected inmouse brain tissue, Jurkat cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55245-1-AP targets HCN2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name hyperpolarization activated cyclic nucleotide-gated potassium channel 2
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 97-110 kDa
GenBank Accession NumberNM_001194
Gene Symbol HCN2
Gene ID (NCBI) 610
RRIDAB_10951108
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HCN2, also named as BCNG2, belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). HCN2 produces a large instantaneous current. It is activated by cAMP. HCN2 is modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. This antibody is specific to HCN2.

Protocols

Product Specific Protocols
WB protocol for HCN2 antibody 55245-1-APDownload protocol
IHC protocol for HCN2 antibody 55245-1-APDownload protocol
IF protocol for HCN2 antibody 55245-1-APDownload protocol
IP protocol for HCN2 antibody 55245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nucleic Acids Res

Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts.

Authors - Donghong Zhang
ratWB

Mol Pain

HCN2 contributes to oxaliplatin-induced neuropathic pain through activation of the CaMKII/CREB cascade in spinal neurons.

Authors - Xiaoyu Liu
ratWB,IHC

Brain Res Bull

HCN2 contributes to oxaliplatin-induced neuropathic pain by inducing spinal long-term potentiation via activation of NMDA receptor-mediated CaMKII signaling.

Authors - Xiaoyu Liu
mouseWB

Exp Ther Med

Effect of mHCN2 gene modification on chronotropic relevant receptors in BMSCs co-cultured with atrial myocytes.

Authors - Chandong Ding
mouseWB

Cell Rep

Optogenetic Activation of Striatopallidal Neurons Reveals Altered HCN Gating in DYT1 Dystonia.

Authors - Giuseppe Sciamanna
mouseWB

Neurosci Bull

Thalamocortical Circuit Controls Neuropathic Pain via Up-regulation of HCN2 in the Ventral Posterolateral Thalamus

Authors - Yi Yan
  • KD Validated