|Positive WB detected in||mouse brain tissue, Jurkat cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human heart tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
55245-1-AP targets HCN2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||hyperpolarization activated cyclic nucleotide-gated potassium channel 2|
|Calculated molecular weight||97 kDa|
|Observed molecular weight||97-110 kDa|
|GenBank accession number||NM_001194|
|Gene ID (NCBI)||610|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
HCN2, also named as BCNG2, belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). HCN2 produces a large instantaneous current. It is activated by cAMP. HCN2 is modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. This antibody is specific to HCN2.
Optogenetic Activation of Striatopallidal Neurons Reveals Altered HCN Gating in DYT1 Dystonia.
Exp Ther Med
Effect of mHCN2 gene modification on chronotropic relevant receptors in BMSCs co-cultured with atrial myocytes.
Brain Res Bull
HCN2 contributes to oxaliplatin-induced neuropathic pain by inducing spinal long-term potentiation via activation of NMDA receptor-mediated CaMKII signaling.
HCN2 contributes to oxaliplatin-induced neuropathic pain through activation of the CaMKII/CREB cascade in spinal neurons.
Nucleic Acids Res
Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts.