HCN2 Polyclonal antibody
HCN2 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 55245-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, Jurkat cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human heart tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 6 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
Product Information
55245-1-AP targets HCN2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | hyperpolarization activated cyclic nucleotide-gated potassium channel 2 |
Calculated molecular weight | 97 kDa |
Observed molecular weight | 97-110 kDa |
GenBank accession number | NM_001194 |
Gene symbol | HCN2 |
Gene ID (NCBI) | 610 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HCN2, also named as BCNG2, belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). HCN2 produces a large instantaneous current. It is activated by cAMP. HCN2 is modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. This antibody is specific to HCN2.
Protocols
Product Specific Protocols | |
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WB protocol for HCN2 antibody 55245-1-AP | Download protocol |
IHC protocol for HCN2 antibody 55245-1-AP | Download protocol |
IP protocol for HCN2 antibody 55245-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nucleic Acids Res Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts. | ||
Mol Pain HCN2 contributes to oxaliplatin-induced neuropathic pain through activation of the CaMKII/CREB cascade in spinal neurons. | ||
Brain Res Bull HCN2 contributes to oxaliplatin-induced neuropathic pain by inducing spinal long-term potentiation via activation of NMDA receptor-mediated CaMKII signaling. | ||
Exp Ther Med Effect of mHCN2 gene modification on chronotropic relevant receptors in BMSCs co-cultured with atrial myocytes. | ||
Cell Rep Optogenetic Activation of Striatopallidal Neurons Reveals Altered HCN Gating in DYT1 Dystonia. | ||
Neurosci Bull Thalamocortical Circuit Controls Neuropathic Pain via Up-regulation of HCN2 in the Ventral Posterolateral Thalamus
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