HDAC1 Monoclonal antibody
HDAC1 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
human, mouse, rat
WB, IHC, IF, ChIP, ELISA
Cat no : 66085-1-Ig
Validation Data Gallery
|Positive WB detected in||HeLa cells|
|Positive IHC detected in||human lymphoma tissue, human lung cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A431 cells, HeLa cells|
|Western Blot (WB)||WB : 1:20000-1:100000|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:10000|
|Immunofluorescence (IF)||IF : 1:6000-1:24000|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 7 publications below|
|IHC||See 1 publications below|
|ChIP||See 1 publications below|
The immunogen of 66085-1-Ig is HDAC1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||HDAC1 fusion protein Ag19128|
|Full Name||histone deacetylase 1|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||65 kDa|
|GenBank accession number||BC000301|
|Gene ID (NCBI)||3065|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is raised against residues near the C terminus of human HDAC1. The calculated molecular weight of HDAC1 is 55 kDa, but modified HDAC1 is about 60-65 kDa .
|Product Specific Protocols|
|WB protocol for HDAC1 antibody 66085-1-Ig||Download protocol|
|IHC protocol for HDAC1 antibody 66085-1-Ig||Download protocol|
|IF protocol for HDAC1 antibody 66085-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Histone Deacetylases 1 and 2 Regulate Microglia Function during Development, Homeostasis, and Neurodegeneration in a Context-Dependent Manner.
J Clin Invest
The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.
ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin.
Br J Pharmacol
Physalin B attenuates liver fibrosis via suppressing LAP2α-HDAC1-mediated deacetylation of the transcription factor GLI1 and hepatic stellate cell activation.
Front Cell Dev Biol
LncRNA NORAD Promotes Vascular Endothelial Cell Injury and Atherosclerosis Through Suppressing VEGF Gene Transcription via Enhancing H3K9 Deacetylation by Recruiting HDAC6.
10-Hydroxydec-2-Enoic Acid Reduces Hydroxyl Free Radical-Induced Damage to Vascular Smooth Muscle Cells by Rescuing Protein and Energy Metabolism.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Andrea (Verified Customer) (01-11-2023)