HDAC1 Monoclonal antibody

HDAC1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

9

CloneNo.

4C4G7

Reviews (1)

Cat no : 66085-1-Ig

Synonyms

GON 10, HD1, HDAC1, histone deacetylase 1, RPD3, RPD3L1

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Validation Data Gallery

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  WB analysis of HeLa using 66085-1-Ig

  Western blot analysis of HDAC1 in various cell lines using Proteintech antibody 66085-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66085-1-Ig (HDAC1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66085-1-Ig (HDAC1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66085-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66085-1-Ig(HDAC1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of A431 using 66085-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using HDAC1 antibody (66085-1-Ig, Clone: 4C4G7 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of A431 using 66085-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using HDAC1 antibody (66085-1-Ig, Clone: 4C4G7 ) at dilution of 1:12000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human lymphoma tissue, human lung cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:6000-1:24000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Product Information

The immunogen of 66085-1-Ig is HDAC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HDAC1 fusion protein Ag19128
• Full Name: histone deacetylase 1
• Calculated molecular weight: 55 kDa
• Observed molecular weight: 65 kDa
• GenBank accession number: BC000301
• Gene symbol: HDAC1
• Gene ID (NCBI): 3065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is raised against residues near the C terminus of human HDAC1. The calculated molecular weight of HDAC1 is 55 kDa, but modified HDAC1 is about 60-65 kDa .

Publications

Species	Application	Title
mouse	WB	Immunity; Histone Deacetylases 1 and 2 Regulate Microglia Function during Development, Homeostasis, and Neurodegeneration in a Context-Dependent Manner.; Authors - Moumita Datta
human	WB	J Clin Invest; The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.; Authors - Wanjin Li
human	WB	Nat Commun; ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin.; Authors - Lifang Li
mouse	WB	Br J Pharmacol; Physalin B attenuates liver fibrosis via suppressing LAP2α-HDAC1-mediated deacetylation of the transcription factor GLI1 and hepatic stellate cell activation.; Authors - Xiaoyun Zhu
human	ChIP	Front Cell Dev Biol; LncRNA NORAD Promotes Vascular Endothelial Cell Injury and Atherosclerosis Through Suppressing VEGF Gene Transcription via Enhancing H3K9 Deacetylation by Recruiting HDAC6.; Authors - Huihua Kai
mouse	WB	Front Nutr; 10-Hydroxydec-2-Enoic Acid Reduces Hydroxyl Free Radical-Induced Damage to Vascular Smooth Muscle Cells by Rescuing Protein and Energy Metabolism.; Authors - Pei Fan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Andrea (Verified Customer) (01-11-2023)

Excellent antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mESCs

HDAC1 Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:66085-1-Ig)