HDAC10 Antibody

HDAC10 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 24913-1-AP

Synonyms

HD10, HDAC10, histone deacetylase 10



Tested Applications

Positive WB detected infetal human brain tissue, mouse thymus tissue
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 24913-1-AP immunogen is Fusion Protein HDAC10 protein expressed in E. coli.

Reactivity human, mouse, rat
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDAC10 fusion protein Ag18470
Full Name histone deacetylase 10
Calculated molecular weight 669aa,71 kDa
Observed molecular weight 68 kDa
GenBank accession number BC125083
Gene symbol HDAC10
Gene ID (NCBI) 83933
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.

Protocols

Product Specific Protocols
WB protocol for HDAC10 antibody 24913-1-APDownload protocol
IHC protocol for HDAC10 antibody 24913-1-APDownload protocol
IF protocol for HDAC10 antibody 24913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncol Lett

HDAC10 expression is associated with DNA mismatch repair gene and is a predictor of good prognosis in colon carcinoma.

Authors - Xiangxiang Tao
mouse, ratWB

Kidney Int

Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.

Authors - Xiaojie Wang