|Positive WB detected in||fetal human brain tissue, mouse thymus tissue|
|Positive IHC detected in||human colon cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The 24913-1-AP immunogen is Fusion Protein HDAC10 protein expressed in E. coli.
|Reactivity||human, mouse, rat|
|Cited Species||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HDAC10 fusion protein Ag18470|
|Full Name||histone deacetylase 10|
|Calculated molecular weight||669aa,71 kDa|
|Observed molecular weight||68 kDa|
|GenBank accession number||BC125083|
|Gene ID (NCBI)||83933|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.
HDAC10 expression is associated with DNA mismatch repair gene and is a predictor of good prognosis in colon carcinoma.
Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.