HDAC2-specific Polyclonal antibody

HDAC2-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16152-1-AP

Synonyms

HDAC2, RPD3, histone deacetylase 2, HD2, EC:3.5.1.98

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 16152-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16152-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16152-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16152-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 16152-1-AP

  IP result of anti-HDAC2-specific (IP:16152-1-AP, 4ug; Detection:16152-1-AP 1:500) with A431 cells lysate 2400ug.

• 

  IHC staining of human testis using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16152-1-AP

  Immunofluorescent analysis of HeLa cells using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

---

Tested Applications

• Positive WB detected in: HepG2 cells, A431 cells, HeLa cells, human liver tissue
• Positive IP detected in: A431 cells
• Positive IHC detected in: human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

16152-1-AP targets HDAC2-specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone deacetylase 2
• Calculated Molecular Weight: 458 aa, 52 kDa; 488 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: NM_001527
• Gene Symbol: HDAC2
• Gene ID (NCBI): 3066
• RRID: AB_2118514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC2 and is specific to HDAC2. It will not cross react with other HDACs.

Publications

Species	Application	Title
human		Hum Reprod; Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility.; Authors - Yi-Min Cheng
human	WB	Chemosphere; Combined exposure of lead and cadmium leads to the aggravated neurotoxicity through regulating the expression of histone deacetylase 2.; Authors - Ruiqing Zhou
human	IF	Leuk Res; High-expressing cystic fibrosis transmembrane conductance regulator interacts with histone deacetylase 2 to promote the development of Ph+ leukemia through the HDAC2-mediated PTEN pathway.; Authors - Tianyou Yan
human	WB	Mol Cell; Histone H3 lysine 27 crotonylation mediates gene transcriptional repression in chromatin; Authors - Nan Liu