|Positive WB detected in||HepG2 cells, A431 cells, HeLa cells, human liver tissue|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
16152-1-AP targets HDAC2-specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Full Name||histone deacetylase 2|
|Calculated molecular weight||458 aa, 52 kDa; 488 aa, 55 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||NM_001527|
|Gene ID (NCBI)||3066|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC2 and is specific to HDAC2. It will not cross react with other HDACs.
|Product Specific Protocols|
|WB protocol for HDAC2-specific antibody 16152-1-AP||Download protocol|
|IHC protocol for HDAC2-specific antibody 16152-1-AP||Download protocol|
|IF protocol for HDAC2-specific antibody 16152-1-AP||Download protocol|
|IP protocol for HDAC2-specific antibody 16152-1-AP||Download protocol|
|Click here to view our Standard Protocols|
High-expressing cystic fibrosis transmembrane conductance regulator interacts with histone deacetylase 2 to promote the development of Ph+ leukemia through the HDAC2-mediated PTEN pathway.
Combined exposure of lead and cadmium leads to the aggravated neurotoxicity through regulating the expression of histone deacetylase 2.
Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility.