HDAC2-specific Polyclonal antibody

HDAC2-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16152-1-AP

Synonyms

HD2, HDAC2, histone deacetylase 2, RPD3, YAF1



Tested Applications

Positive WB detected inHepG2 cells, A431 cells, HeLa cells, human liver tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman testis tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

16152-1-AP targets HDAC2-specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name histone deacetylase 2
Calculated molecular weight 458 aa, 52 kDa; 488 aa, 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberNM_001527
Gene symbol HDAC2
Gene ID (NCBI) 3066
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC2 and is specific to HDAC2. It will not cross react with other HDACs.

Protocols

Product Specific Protocols
WB protocol for HDAC2-specific antibody 16152-1-APDownload protocol
IHC protocol for HDAC2-specific antibody 16152-1-APDownload protocol
IF protocol for HDAC2-specific antibody 16152-1-APDownload protocol
IP protocol for HDAC2-specific antibody 16152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Chemosphere

Combined exposure of lead and cadmium leads to the aggravated neurotoxicity through regulating the expression of histone deacetylase 2.

Authors - Ruiqing Zhou
human

Hum Reprod

Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility.

Authors - Yi-Min Cheng