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- KD/KO Validated
HDAC3-specific Polyclonal antibody
HDAC3-specific Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Cat no : 16164-1-AP
|Positive WB detected in||HEK-293T cells, HEK-293 cells, human stomach tissue, HeLa cells, HL-60 cells, MOLT-4 cells, NCI-H1299 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Sample-dependent, check data in validation data gallery|
16164-1-AP targets HDAC3-specific in WB, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Full Name||histone deacetylase 3|
|Calculated molecular weight||49 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||NM_003883|
|Gene ID (NCBI)||8841|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
What is the molecular weight of HDAC3? Is HDAC3 post-translationally modified?
The molecular weight of HDAC3 is 49 kDa but HDAC3 can form homodimers and homotrimers in cells (PMID: 11779848). HDAC3 can be phosphorylated at S424 residue by protein kinase CK2, which regulates HDAC3 activity (PMID: 15805470).
What is the subcellular localization of HDAC3?
Unlike other class I HDACs, HDAC3 actively shuttles between the nucleus and cytoplasm (PMID: 11779848). Additionally, HDAC3 can be present at the plasma membrane, where it associates with c-Src (PMID: 16532030).
What is the tissue expression pattern of HDAC3?
Like other class I HDACs, HDAC3 is ubiquitously expressed (PMID: 9346952).
What is the mechanism of HDAC3 in regulating gene expression?
Unlike other histone deacetylates, it exists in a complex with two proteins - nuclear receptor co-repressor (N-CoR) and silencing mediator for retinoid and thyroid receptors (SMRT) - and therefore acts as a mediator of the repression function of unliganded nuclear hormone receptors (PMID: 10809664). N-CoR and SMRT play an active role in the recruitment of HDAC3 to binding sites as well as directly regulating HDAC3 activity. Additionally, HDAC3 targets growth-regulated genes.