HDAC4 Monoclonal antibody

HDAC4 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

CloneNo.

1D2G12

Cat no : 66838-1-Ig

Synonyms

HA6116, HD4, HDAC A, HDAC4, HDACA, histone deacetylase 4, KIAA0288



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

66838-1-Ig targets HDAC4 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HDAC4 fusion protein Ag11345
Full Name histone deacetylase 4
Calculated molecular weight 972 aa, 106 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC039904
Gene symbol HDAC4
Gene ID (NCBI) 9759
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

Protocols

Product Specific Protocols
WB protocol for HDAC4 antibody 66838-1-IgDownload protocol
IHC protocol for HDAC4 antibody 66838-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP,ChIP

Appl Physiol Nutr Metab

Exercise improves high-fat diet-induced metabolic disorder by promoting HDAC5 degradation through the ubiquitin-proteasome system in skeletal muscle

Authors - Song Huang
  • KD Validated
humanIF

J Mol Cell Cardiol

Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation.

Authors - Yongyun Li
humanIF

Virulence

Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.

Authors - Wei Jie
humanWB

Oncogenesis

USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.

Authors - Wei Zhan