HDAC4 Monoclonal antibody

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  WB analysis using 66838-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66838-1-Ig (HDAC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66838-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66838-1-Ig (HDAC4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66838-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66838-1-Ig (HDAC4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 66838-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using HDAC4 antibody (66838-1-Ig, Clone: 1D2G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of C2C12 using 66838-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using HDAC4 antibody (66838-1-Ig, Clone: 1D2G12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

HDAC4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 66838-1-Ig

Clone No.1D2G12

Publications(7)

Reviews (1)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA

Synonyms

KIAA0288, HD4, EC:3.5.1.98, 1D2G12

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue
• Positive IF/ICC detected in: C2C12 cells

This antibody is not recommended for WB assays of mouse samples.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

66838-1-Ig targets HDAC4 in WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HDAC4 fusion protein Ag11345
• Full Name: histone deacetylase 4
• Calculated Molecular Weight: 972 aa, 106 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC039904
• Gene Symbol: HDAC4
• Gene ID (NCBI): 9759
• RRID: AB_2882181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P56524
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

Publications

Species	Application	Title
human	WB	Oncogenesis; USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.; Authors - Wei Zhan
human	IF	Virulence; Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.; Authors - Wei Jie
human	IF	J Mol Cell Cardiol; Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation.; Authors - Yongyun Li
mouse	WB,IP,ChIP	Appl Physiol Nutr Metab; Exercise improves high-fat diet-induced metabolic disorder by promoting HDAC5 degradation through the ubiquitin-proteasome system in skeletal muscle; Authors - Song Huang ; KD Validated
mouse	WB	J Toxicol Sci; Propofol improves ischemia reperfusion-induced liver fibrosis by regulating lncRNA HOXA11-AS; Authors - Jia Luo ; KD Validated
human,mouse	WB	Adv Clin Exp Med; Tasquinimod enhances the sensitivity of ovarian cancer cells to cisplatin by regulating the Nur77-Bcl-2 apoptotic pathway; Authors - Ying Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shinford (Verified Customer) (10-26-2023)

In our screening of basic expression in lung, breast, and gastric cancer cell lines, antibody concentrations ranging from 1:1000 to 2000 all displayed clear bands, indicating stable working conditions.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Multiple cell lines of lung cancer, breast cancer, gastric cancer.

HDAC4 Antibody Western Blot validation (1:1000 dilution) in Multiple cell lines of lung cancer, breast cancer, gastric cancer. (Cat no:66838-1-Ig)