Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
| Positive IF/ICC detected in | C2C12 cells |
This antibody is not recommended for WB assays of mouse samples.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66838-1-Ig targets HDAC4 in WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11345 Product name: Recombinant human HDAC4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 623-972 aa of BC039904 Sequence: SKKLLGSLASVFVRLPCGGVGVDSDTIWNEVHSAGAARLAVGCVVELVFKVATGELKNGFAVVRPPGHHAEESTPMGFCYFNSVAVAAKLLQQRLSVSKILIVDWDVHHGNGTQQAFYSDPSVLYMSLHRYDDGNFFPGSGAPDEVGTGPGVGFNVNMAFTGGLDPPMGDAEYLAAFRTVVMPIASEFAPDVVLVSSGFDAVEGHPTPLGGYNLSARCFGYLTKQLMGLAGGRIVLALEGGHDLTAICDASEACVSALLGNELDPLPEKVLQQRPNANAVRSMEKVMEIHSKYWRCLQRTTSTAGRSLIEAQTCENEEAETVTAMASLSVGVKPAEKRPDEEPMEEEPPL Predict reactive species |
| Full Name | histone deacetylase 4 |
| Calculated Molecular Weight | 972 aa, 106 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC039904 |
| Gene Symbol | HDAC4 |
| Gene ID (NCBI) | 9759 |
| RRID | AB_2882181 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P56524 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HDAC4 antibody 66838-1-Ig | Download protocol |
| IHC protocol for HDAC4 antibody 66838-1-Ig | Download protocol |
| IP protocol for HDAC4 antibody 66838-1-Ig | Download protocol |
| WB protocol for HDAC4 antibody 66838-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HDAC4 Monoclonal antibody (1D2G12), catalog number 66838-1-Ig, has 10 citations spanning journals including Research, Virulence, Cell Mol Life Sci, Oncogenesis, Stem Cell Reports, J Mol Cell Cardiol, J Biochem Mol Toxicol, Adv Clin Exp Med, Appl Physiol Nutr Metab, and J Toxicol Sci. Research fields cover epigenetics, virology, depression, cancer, osteogenesis, cardiovascular biology, toxicology, metabolic disorders, and liver fibrosis.
| Species | Application | Title |
|---|---|---|
Research (Wash D C) Histone Lactylation Couples FSH-Driven Lactate Metabolism to Mitochondrial Biogenesis by Enhancing HDAC4-Mediated Deacetylation of PGC-1α in Granulosa Cells. | ||
Virulence Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions. | ||
Cell Mol Life Sci Upregulation of HDAC9 in hippocampal neurons mediates depression-like behaviours by inhibiting ANXA2 degradation | ||
Oncogenesis USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3. | ||
Stem Cell Reports HDAC4 regulates hASC osteogenesis and bone regeneration by mediating SMAD4 via histone acetylation. | ||
J Mol Cell Cardiol Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation. |
Reviews
What customers say
The single review for 66838-1-Ig is a 5-star rating from a Western Blot application. The reviewer screened basic expression across multiple lung, breast, and gastric cancer cell lines, using a 1:1000 dilution. They reported that antibody concentrations from 1:1000 to 1:2000 all produced clear bands, indicating stable and reliable working conditions. Overall, the feedback is positive, highlighting consistent performance and clear signal across diverse cancer cell lines.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shinford (Verified Customer) (10-26-2023) | In our screening of basic expression in lung, breast, and gastric cancer cell lines, antibody concentrations ranging from 1:1000 to 2000 all displayed clear bands, indicating stable working conditions.
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