HDAC4 Monoclonal antibody
HDAC4 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Human and More (2)
WB, IP, IHC, IF, ChIP, ELISA
Cat no : 66838-1-Ig
|Positive WB detected in||HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 2 publications below|
|IF||See 2 publications below|
|IP||See 1 publications below|
|ChIP||See 1 publications below|
66838-1-Ig targets HDAC4 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||HDAC4 fusion protein Ag11345|
|Full Name||histone deacetylase 4|
|Calculated molecular weight||972 aa, 106 kDa|
|Observed molecular weight||140 kDa|
|GenBank accession number||BC039904|
|Gene ID (NCBI)||9759|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
|Product Specific Protocols|
|WB protocol for HDAC4 antibody 66838-1-Ig||Download protocol|
|IHC protocol for HDAC4 antibody 66838-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.
Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.
J Mol Cell Cardiol
Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation.
Appl Physiol Nutr Metab
Exercise improves high-fat diet-induced metabolic disorder by promoting HDAC5 degradation through the ubiquitin-proteasome system in skeletal muscle