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HDAC6 Antibody 7 Publications

Rabbit Polyclonal| Catalog number: 12834-1-AP

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Con: 60 μg/150 μl

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Tested Applications
Applications:
ELISA, WB, IP, IHC, IF
Application not tested? Please see our Guarantee


Images See all

12834-1-AP;K-562 cells

K-562 cells were subjected to SDS PAGE followed by western blot with 12834-1-AP(HDAC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

12834-1-AP;K-562 cells

IP Result of anti-HDAC6 (IP:12834-1-AP, 5ug; Detection:12834-1-AP 1:1000) with K-562 cells lysate 11000ug.

12834-1-AP;human testis tissue

Immunohistochemical of paraffin-embedded human testis using 12834-1-AP(HDAC6 antibody) at dilution of 1:50 (under 10x lens)

12834-1-AP;human testis tissue

Immunohistochemical of paraffin-embedded human testis using 12834-1-AP(HDAC6 antibody) at dilution of 1:50 (under 40x lens)

12834-1-AP;HepG2 cells

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 12834-1-AP(HDAC6 antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
K-562 cells, HeLa cells, HepG2 cells, HT-1080 cells, human liver tissue, MCF-7 cells
Positive IP detected in:
K-562 cells
Positive IHC detected in:
human testis tissue
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
HDAC6 fusion protein ag3572
Full name:
histone deacetylase 6
Calculated molecular weight:
1063aa,114 kDa,131 kDa
Observed molecular weight:
160 kDa
GenBank accession number:
BC013737
Gene ID (NCBI):
10013
Gene symbol
HDAC6
Synonyms
FLJ16239, HD6, HDAC6, histone deacetylase 6, JM21, KIAA0901
Background

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is a rabbit polyclonal antibody raised against residues near the C terminal of human HDAC6.


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