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HDAC6 Antibody 16 Publications

Rabbit Polyclonal| Catalog number: 12834-1-AP

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Con: 60 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

HDAC6 Antibody WB K-562 cells 12834-1-AP

K-562 cells were subjected to SDS PAGE followed by western blot with 12834-1-AP(HDAC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HDAC6 Antibody IP K-562 cells 12834-1-AP

IP Result of anti-HDAC6 (IP:12834-1-AP, 5ug; Detection:12834-1-AP 1:1000) with K-562 cells lysate 11000ug.

HDAC6 Antibody IHC human testis tissue 12834-1-AP

Immunohistochemical analysis of paraffin-embedded human testis using 12834-1-AP(HDAC6 antibody) at dilution of 1:50 (under 10x lens)

HDAC6 Antibody IHC human testis tissue 12834-1-AP

Immunohistochemical analysis of paraffin-embedded human testis using 12834-1-AP(HDAC6 antibody) at dilution of 1:50 (under 40x lens)

HDAC6 Antibody IF HepG2 cells 12834-1-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 12834-1-AP(HDAC6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
K-562 cells, human liver tissue, HeLa cells, HepG2 cells
Positive IP detected in:
K-562 cells
Positive IHC detected in:
human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
HDAC6 fusion protein Ag3572
Full name:
histone deacetylase 6
Calculated molecular weight:
1063aa,114 kDa,131 kDa
Observed molecular weight:
160 kDa
GenBank accession number:
BC013737
Gene ID (NCBI):
10013
Gene symbol
HDAC6
Synonyms
HD6, HDAC6, histone deacetylase 6, JM21, KIAA0901

Background

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is a rabbit polyclonal antibody raised against residues near the C terminal of human HDAC6. The calculated molecular weight of HDAC6 is 130 kDa, but the modified the HDAC6 is about 150-160 kDa.


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