Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, human liver tissue, Calu-1 cells, L02 cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human testis tissue, human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MG132 treated HepG2 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12834-1-AP targets HDAC6 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3572 Product name: Recombinant human HDAC6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 712-1064 aa of BC013737 Sequence: AERMTTREKKVLEAGMGKVTSASFGEESTPGQTNSETAVVALTQDQPSEAATGGATLAQTISEAAIGGAMLGQTTSEEAVGGATPDQTTSEETVGGAILDQTTSEDAVGGATLGQTTSEEAVGGATLAQTTSEAAMEGATLDQTTSEEAPGGTELIQTPLASSTDHQTPPTSPVQGTTPQISPSTLIGSLRTLELGSESQGASESQAPGEENLLGEAAGGQDMADSMLMQGSRGLTDQAIFYAVTPLPWCPHLVAVCPIPAAGLDVTQPCGDCGTIQENWVCLSCYQVYCGRYINGHMLQHHGNSGHPLVLSYIDLSAWCYYCQAYVHHQALLDVKNIAHQNKFGEDMPHPH Predict reactive species |
| Full Name | histone deacetylase 6 |
| Calculated Molecular Weight | 1063 aa, 114 kDa, 131 kDa |
| Observed Molecular Weight | 160 kDa |
| GenBank Accession Number | BC013737 |
| Gene Symbol | HDAC6 |
| Gene ID (NCBI) | 10013 |
| RRID | AB_10597094 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBN7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is a rabbit polyclonal antibody raised against residues near the C terminal of human HDAC6. The calculated molecular weight of HDAC6 is 130 kDa, but the modified the HDAC6 is about 150-160 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HDAC6 antibody 12834-1-AP | Download protocol |
| IHC protocol for HDAC6 antibody 12834-1-AP | Download protocol |
| IP protocol for HDAC6 antibody 12834-1-AP | Download protocol |
| WB protocol for HDAC6 antibody 12834-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HDAC6 Polyclonal antibody (Cat# 12834-1-AP) has accumulated 93 citations in journals including Nature, Nat Commun, Cancer Res, Mol Cancer, Cell Death Dis, Oncogene, FASEB J, Elife, and Nucleic Acids Res. Research fields span oncology (lung, liver, breast, colon, glioblastoma), neurodegeneration (Parkinson's, Alzheimer's, ALS), epigenetics, cell signaling, metabolism, cardiovascular disease, infectious disease, and developmental biology. Cited applications include WB, IHC, IF, IP, CoIP, ChIP, and RIP.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther The noncanonical function of liver-type phosphofructokinase potentiates the efficacy of HDAC inhibitors in cancer. | ||
Mol Cancer HDAC6 orchestrates metastatic and immunosuppressive programs in small cell lung cancer through S100A2-TGF-β/SMAD and CSF1R signaling.
| ||
Bioact Mater Local delivery of EGFR+NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway | ||
Cancer Res Stabilized peptide HDAC inhibitors derived from HDAC1 substrate H3K56 for the treatment of cancer stem-like cells in vivo. | ||
Kidney Int Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy. |
Reviews
What customers say
Both reviewers rated this antibody 5 stars. One user reported that HDAC6 produces very sharp bands in Western Blot at a 1:1000 dilution. Another user successfully used it for both Western Blot and Immunofluorescence on human liver cells (HCC cell lines) at 1:1000, detecting the expected band after overnight incubation at 4°C with ECL imaging. Overall, users are satisfied with the antibody's specificity and performance across applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sai Sindhura (Verified Customer) (01-08-2026) | HDAC6 gives very sharp bands
|
K (Verified Customer) (08-03-2021) | total cell lysate (20 ug of human HCC cell lines) was resolved on 10% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% BSA in TBS/0.1% Tween-20) for 1h. Membrane was incubated with this in blocking buffer (1:1000) at 40C overnight. After 1h incubation with appropriate secondary antibody (anti-Rabbit 1:1000) membranes were imaged with ECL and expected band was detected.
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