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  • KD/KO Validated

HDAC6 Polyclonal antibody

HDAC6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12834-1-AP

Synonyms

HD6, HDAC6, histone deacetylase 6, JM21, KIAA0901



Tested Applications

Positive WB detected inK-562 cells, human liver tissue, HeLa cells, HepG2 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12834-1-AP targets HDAC6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDAC6 fusion protein Ag3572
Full Name histone deacetylase 6
Calculated molecular weight 1063 aa, 114 kDa, 131 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC013737
Gene symbol HDAC6
Gene ID (NCBI) 10013
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is a rabbit polyclonal antibody raised against residues near the C terminal of human HDAC6. The calculated molecular weight of HDAC6 is 130 kDa, but the modified the HDAC6 is about 150-160 kDa.

Protocols

Product Specific Protocols
WB protocol for HDAC6 antibody 12834-1-APDownload protocol
IHC protocol for HDAC6 antibody 12834-1-APDownload protocol
IF protocol for HDAC6 antibody 12834-1-APDownload protocol
IP protocol for HDAC6 antibody 12834-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Thorac Cardiovasc Surg

Histone deacetylase 6 reduction promotes aortic valve calcification via an endoplasmic reticulum stress-mediated osteogenic pathway.

Authors - Zurong Fu
  • KD Validated
mouseWB

Development

Rab23/Kif17 regulate meiotic progression in oocytes by modulating tubulin acetylation and actin dynamics.

Authors - Hong-Hui Wang
humanWB

Cancer Res

Stabilized peptide HDAC inhibitors derived from HDAC1 substrate H3K56 for the treatment of cancer stem-like cells in vivo.

Authors - Dongyuan Wang
humanWB,IHC

Life Sci

Histone deacetylases and acetylated histone H3 are involved in the process of hepatitis B virus DNA replication.

Authors - Di Zhang
ratWB

Small

Key Role of Microtubule and Its Acetylation in a Zinc Oxide Nanoparticle-Mediated Lysosome-Autophagy System.

Authors - Jia Liu
mouseWB

Mol Hum Reprod

RAB35 depletion affects spindle formation and actin-based spindle migration in mouse oocyte meiosis.

Authors - Yu Zhang