HDAC6 Polyclonal antibody

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  WB analysis of K-562 using 12834-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12834-1-AP (HDAC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12834-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12834-1-AP (HDAC6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12834-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12834-1-AP (HDAC6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12834-1-AP

  WB result of HDAC6 antibody (12834-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDAC6 transfected HeLa cells.

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  WB analysis of HepG2 using 12834-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12834-1-AP (HDAC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12834-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12834-1-AP (HDAC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12834-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12834-1-AP (HDAC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12834-1-AP

  IP result of anti-HDAC6 (IP:12834-1-AP, 5ug; Detection:12834-1-AP 1:1000) with K-562 cells lysate 11000ug.

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  IHC staining of human testis using 12834-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12834-1-AP (HDAC6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12834-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12834-1-AP (HDAC6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 12834-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12834-1-AP (HDAC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12834-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12834-1-AP (HDAC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12834-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12834-1-AP (HDAC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12834-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MG132 treated HepG2 cells using HDAC6 antibody (12834-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12834-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 12834-1-AP (HDAC6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

HDAC6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12834-1-AP

Publications(68)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

Protein deacetylase HDAC6, KIAA0901, Histone deacetylase 6, HDAC 6, EC:3.5.1.98

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, human liver tissue, HepG2 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human testis tissue, human breast cancer tissue, human lung cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MG132 treated HepG2 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 60 publications below
• IHC: See 6 publications below
• IF: See 15 publications below
• IP: See 2 publications below
• CoIP: See 4 publications below
• RIP: See 1 publications below

Product Information

12834-1-AP targets HDAC6 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDAC6 fusion protein Ag3572
• Full Name: histone deacetylase 6
• Calculated Molecular Weight: 1063 aa, 114 kDa, 131 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC013737
• Gene Symbol: HDAC6
• Gene ID (NCBI): 10013
• RRID: AB_10597094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UBN7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is a rabbit polyclonal antibody raised against residues near the C terminal of human HDAC6. The calculated molecular weight of HDAC6 is 130 kDa, but the modified the HDAC6 is about 150-160 kDa.

Publications

Species	Application	Title
	IF,WB	Bioact Mater; Local delivery of EGFR+NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway; Authors - Tian Qin
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
human	WB,IP	Genome Biol; The histone modification reader ZCWPW1 promotes double-strand break repair by regulating cross-talk of histone modifications and chromatin accessibility at meiotic hotspots; Authors - Shenli Yuan
human	WB,IP	Oncogene; HDAC6-G3BP2 promotes lysosomal-TSC2 and suppresses mTORC1 under ETV4 targeting-induced low-lactate stress in non-small cell lung cancer; Authors - Bei Liu ; KD Validated
	WB	Kidney Int; Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.; Authors - Xiaojie Wang
human	WB,IHC	Biomed Pharmacother; Degradation of histone deacetylase 6 alleviates ROS-mediated apoptosis in renal ischemia-reperfusion injury; Authors - Kang Xia

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

K (Verified Customer) (08-03-2021)

total cell lysate (20 ug of human HCC cell lines) was resolved on 10% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% BSA in TBS/0.1% Tween-20) for 1h. Membrane was incubated with this in blocking buffer (1:1000) at 40C overnight. After 1h incubation with appropriate secondary antibody (anti-Rabbit 1:1000) membranes were imaged with ECL and expected band was detected.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human liver cells

HDAC6 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in human liver cells (Cat no:12834-1-AP)