- Featured Product
- KD/KO Validated
HEXIM1 Polyclonal antibody
HEXIM1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 15676-1-AP
Validation Data Gallery
|Positive WB detected in
|HeLa cells, MCF-7 cells
|Positive IP detected in
|Positive IHC detected in
|human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:6000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
15676-1-AP targets HEXIM1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|HEXIM1 fusion protein Ag8144
|hexamethylene bis-acetamide inducible 1
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).
|Product Specific Protocols
|WB protocol for HEXIM1 antibody 15676-1-AP
|IHC protocol for HEXIM1 antibody 15676-1-AP
|IF protocol for HEXIM1 antibody 15676-1-AP
|IP protocol for HEXIM1 antibody 15676-1-AP
|FC protocol for HEXIM1 antibody 15676-1-AP
|Click here to view our Standard Protocols
Divergent transcriptional regulation of astrocyte reactivity across disorders.
Adv Sci (Weinh)
cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.
Positive transcription elongation factor b activity in compensatory myocardial hypertrophy is regulated by cardiac lineage protein-1.
Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.
Nucleic Acids Res
HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters.
Interaction of the Ankyrin H Core Effector of Legionella with the Host LARP7 Component of the 7SK snRNP Complex.