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HEXIM1 Polyclonal antibody

HEXIM1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15676-1-AP

Synonyms

Cardiac lineage protein 1, CLP1, EDG1, HEXIM1, HIS1, MAQ1, Menage a quatre protein 1, Protein HEXIM1



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

15676-1-AP targets HEXIM1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HEXIM1 fusion protein Ag8144
Full Name hexamethylene bis-acetamide inducible 1
Calculated molecular weight 41 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC006460
Gene symbol HEXIM1
Gene ID (NCBI) 10614
RRIDAB_2248363
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).

Protocols

Product Specific Protocols
WB protocol for HEXIM1 antibody 15676-1-APDownload protocol
IHC protocol for HEXIM1 antibody 15676-1-APDownload protocol
IF protocol for HEXIM1 antibody 15676-1-APDownload protocol
IP protocol for HEXIM1 antibody 15676-1-APDownload protocol
FC protocol for HEXIM1 antibody 15676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
humanIF

Adv Sci (Weinh)

cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.

Authors - Zeyun Mi
mouseWB,IP

Circ Res

Positive transcription elongation factor b activity in compensatory myocardial hypertrophy is regulated by cardiac lineage protein-1.

Authors - Espinoza-Derout Jorge J
humanIHC

Acta Neuropathol

Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.

Authors - Neumann Manuela M
humanWB

Nucleic Acids Res

HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters.

Authors - Sebastian Eilebrecht
humanWB

MBio

Interaction of the Ankyrin H Core Effector of Legionella with the Host LARP7 Component of the 7SK snRNP Complex.

Authors - Juanita Von Dwingelo