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HEY2 Antibody

HEY2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Cat no : 10597-1-AP

Synonyms

bHLHb32, CHF1, GRIDLOCK, GRL, hCHF1, HERP, HERP1, HESR 2, HESR2, HEY2, hHRT2, HRT 2, HRT2, Protein gridlock homolog



Tested Applications

Positive WB detected inK-562 cells, mouse heart tissue, MCF-7 cells, 4T1 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10597-1-AP targets HEY2 in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HEY2 fusion protein Ag0913
Full Name hairy/enhancer-of-split related with YRPW motif 2
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession number BC007707
Gene symbol HEY2
Gene ID (NCBI) 23493
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system. This antibody is a rabbit polyclonal antibody raised against full length of human HEY2

Protocols

Product Specific Protocols
WB protocol for HEY2 antibody 10597-1-APDownload protocol
IHC protocol for HEY2 antibody 10597-1-APDownload protocol
IF protocol for HEY2 antibody 10597-1-APDownload protocol
IP protocol for HEY2 antibody 10597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS One

Placental P-glycoprotein inhibition enhances susceptibility to Di-(2-ethylhexyl)-phthalate induced cardiac malformations in mice: A possibly promising target for congenital heart defects prevention.

Authors - Changqing Tang
mouseIHC

Cell Mol Gastroenterol Hepatol

Pancreatic ductal deletion of Hnf1b disrupts exocrine homeostasis, leads to pancreatitis and facilitates tumorigenesis.

Authors - Evans Quilichini
humanWB

Oncotarget

miR-150 enhances apoptotic and anti-tumor effects of paclitaxel in paclitaxel-resistant ovarian cancer cells by targeting Notch3.

Authors - Tae Hoen Kim
humanIF

Cell Stem Cell

Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts.

Authors - Blair K Gage
humanWB

J Hypertens

2B.06: EPIGALLOCATECHIN-3-GALLATE ATTENUATES URIC ACID-INDUCED INFLAMMATORY RESPONSES AND OXIDATIVE STRESS BY MODULATING NOTCH PATHWAY.

Authors - H Xie
mouseWB

J Appl Toxicol

The effect of maternal exposure to di-(2-ethylhexyl)-phthalate on fetal cardiac development in mice.

Authors - Changqing Tang