Tested Applications
| Positive WB detected in | K-562 cells, mouse heart tissue, MCF-7 cells, 4T1 cells |
| Positive IP detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10597-1-AP targets HEY2 in WB, IHC, IF, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0913 Product name: Recombinant human HEY2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-337 aa of BC007707 Sequence: MKRPCEETTSESDMDETIDVGSENNYSGQSTSSVIRLNSPTTTSQIMARKKRRGIIEKRRRDRINNSLSELRRLVPTAFEKQGSAKLEKAEILQMTVDHLKMLQATGGKGYFDAHALAMDFMSIGFRECLTEVARYLSSVEGLDSSDPLRVRLVSHLSTCATQREAAAMTSSMAHHHHPLHPHHWAAAFHHLPAALLQPNGLHASESTPCRLSTTSEVPPAHGSALLTATFAHADSALRMPSTGSVAPCVPPLSTSLLSLSATVHAAAAAATAAAHSFPLSFAGAFPMLPPNAAAAVAAATAISPPLSVSATSSPQQTSSGTNNKPYRPWGTEVGAF Predict reactive species |
| Full Name | hairy/enhancer-of-split related with YRPW motif 2 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 33-40 kDa |
| GenBank Accession Number | BC007707 |
| Gene Symbol | HEY2 |
| Gene ID (NCBI) | 23493 |
| RRID | AB_2118415 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBP5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system. This antibody is a rabbit polyclonal antibody raised against full length of human HEY2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HEY2 antibody 10597-1-AP | Download protocol |
| IP protocol for HEY2 antibody 10597-1-AP | Download protocol |
| WB protocol for HEY2 antibody 10597-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HEY2 Polyclonal antibody (Cat# 10597-1-AP) has 54 citations across journals including Cell, Nature Communications, PNAS, Development, Blood, Cell Stem Cell, Oncogene, and Current Biology. Research fields span Notch signaling, cardiac development, vascular biology, cancer (breast, ovarian, lung, glioma), neurodevelopment, stem cell differentiation, osteogenesis, retinal angiogenesis, and inflammatory responses.
| Species | Application | Title |
|---|---|---|
Blood Notch promotes vascular maturation by inducing integrin-mediated smooth muscle cell adhesion to the endothelial basement membrane. | ||
Cell Stem Cell Modeling human multi-lineage heart field development with pluripotent stem cells | ||
Cell Stem Cell Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts. | ||
Nat Neurosci Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination. | ||
Nat Commun The transcriptional repressor HEY2 regulates mitochondrial oxidative respiration to maintain cardiac homeostasis |
Reviews
What customers say
All three reviews rated this Hey2 antibody 5 stars. For Western blot on mouse heart tissue at 1:1000 dilution, users reported a specific band at the expected ~36 kDa size, though one noted the band was somewhat weak. For immunofluorescence on frozen mouse embryonic heart sections at 1:100 dilution, the antibody produced clear nuclear staining in compact myocardium, consistent with Hey2's known expression pattern. Overall, reviewers found it reliable for both WB and IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
CHAO (Verified Customer) (03-31-2022) | Weak band around correct size.
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Tom (Verified Customer) (08-22-2020) | I performed western blot with mouse heart tissue lysate using Hey2 antibody. A specific ~36 kDa Hey2 band was observed.
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Tom (Verified Customer) (08-22-2020) | This Hey2 antibody is highly recommended for detecting Hey2 on frozen tissue sections! I used this Hey2 antibody for immunofluorescence (IF). It clearly had nuclear staining signal in compact myocardium of embryonic heart, which is consistent with Hey2's well-known expression pattern.
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