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HEY2 Polyclonal antibody

HEY2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10597-1-AP

Synonyms

bHLHb32, CHF1, GRIDLOCK, GRL, hCHF1, HERP, HERP1, HESR 2, HESR2, HEY2, hHRT2, HRT 2, HRT2, Protein gridlock homolog



Tested Applications

Positive WB detected inK-562 cells, mouse heart tissue, MCF-7 cells, 4T1 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10597-1-AP targets HEY2 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HEY2 fusion protein Ag0913
Full Name hairy/enhancer-of-split related with YRPW motif 2
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC007707
Gene symbol HEY2
Gene ID (NCBI) 23493
RRIDAB_2118415
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system. This antibody is a rabbit polyclonal antibody raised against full length of human HEY2

Protocols

Product Specific Protocols
WB protocol for HEY2 antibody 10597-1-APDownload protocol
IHC protocol for HEY2 antibody 10597-1-APDownload protocol
IF protocol for HEY2 antibody 10597-1-APDownload protocol
IP protocol for HEY2 antibody 10597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell

Multi-chamber cardioids unravel human heart development and cardiac defects

Authors - Clara Schmidt
humanIF

Cell Stem Cell

Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts.

Authors - Blair K Gage
mouseWB

Nat Neurosci

Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination.

Authors - Lai Man Natalie Wu
humanchIP

Nat Commun

Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis.

Authors - Jared M Churko
humanIF

Nat Commun

Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells.

Authors - Shunsuke Funakoshi
humanWB

Nat Commun

Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia.

Authors - Haiying Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

CHAO (Verified Customer) (03-31-2022)

Weak band around correct size.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Heart
FH

Tom (Verified Customer) (08-22-2020)

I performed western blot with mouse heart tissue lysate using Hey2 antibody. A specific ~36 kDa Hey2 band was observed.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart
FH

Tom (Verified Customer) (08-22-2020)

This Hey2 antibody is highly recommended for detecting Hey2 on frozen tissue sections! I used this Hey2 antibody for immunofluorescence (IF). It clearly had nuclear staining signal in compact myocardium of embryonic heart, which is consistent with Hey2's well-known expression pattern.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse embryonic heart