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HIF-1 alpha Polyclonal antibody
HIF-1 alpha Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 20960-1-AP
Validation Data Gallery
|Positive WB detected in||Cobalt Chloride treated HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human thyroid cancer tissue, human heart tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Cobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20960-1-AP is HIF-1 alpha Fusion Protein expressed in E. coli.
|Cited Reactivity||human, chicken, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HIF-1 alpha fusion protein Ag15198|
|Full Name||hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|
|Calculated molecular weight||826 aa, 93 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC012527|
|Gene ID (NCBI)||3091|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .
|Product Specific Protocols|
|WB protocol for HIF-1 alpha antibody 20960-1-AP||Download protocol|
|IHC protocol for HIF-1 alpha antibody 20960-1-AP||Download protocol|
|IF protocol for HIF-1 alpha antibody 20960-1-AP||Download protocol|
|IP protocol for HIF-1 alpha antibody 20960-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Carlo (Verified Customer) (01-18-2023)
The quality of the band in western blot is well defined
Davide (Verified Customer) (09-20-2022)
The image uploaded refers to a hypoxic condition O2<1%. The antibody signal was aspecific and generated aspecific binding in the middle of the organoid.
Christian (Verified Customer) (01-07-2022)
Unfortunately much background an many unspecific bands in lysates of human chondrocytes and BV2 microclia cells (less unspecific bands in rat chondrocytes).
Diane (Verified Customer) (02-17-2021)
Separated on a 7.5% SDS PAGE gel, transferred to nitrocellulose membrane. Block with 5% dry milk/PBST at room temperature for one hour. 1:1000 dilution of the primary antibody in blocking solution. Incubated overnight at 4 degrees C on a rocker platform. Secondary antibody 1:1000 secondary (anti rabbit HRP) incubation for two hours at room temperature. Visualization with the Opti-4CN chromogenic substrate kit (BioRad).
Chun (Verified Customer) (12-05-2019)
A good antibody