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  • KD/KO Validated

HIF-1 alpha Polyclonal antibody

HIF-1 alpha Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 20960-1-AP


ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF1, HIF-1, HIF1 ALPHA, HIF-1 alpha, HIF1A, HIF-1α, Member of PAS protein 1, MOP1, PASD8

Tested Applications

Positive WB detected inCobalt Chloride treated HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman thyroid cancer tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20960-1-AP is HIF-1 alpha Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, chicken, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIF-1 alpha fusion protein Ag15198
Full Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Calculated molecular weight 826 aa, 93 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC012527
Gene symbol HIF1A
Gene ID (NCBI) 3091
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .


Product Specific Protocols
WB protocol for HIF-1 alpha antibody 20960-1-APDownload protocol
IHC protocol for HIF-1 alpha antibody 20960-1-APDownload protocol
IF protocol for HIF-1 alpha antibody 20960-1-APDownload protocol
IP protocol for HIF-1 alpha antibody 20960-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Carlo (Verified Customer) (01-18-2023)

The quality of the band in western blot is well defined

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Neuroblastoma cell line

Davide (Verified Customer) (09-20-2022)

The image uploaded refers to a hypoxic condition O2<1%. The antibody signal was aspecific and generated aspecific binding in the middle of the organoid.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cholangiocyte organoids
HIF-1 alpha Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20960-1-AP)

Christian (Verified Customer) (01-07-2022)

Unfortunately much background an many unspecific bands in lysates of human chondrocytes and BV2 microclia cells (less unspecific bands in rat chondrocytes).

  • Applications: Western Blot
  • Cell Tissue Type: human chondrocytes
HIF-1 alpha Antibody Western Blot validation ( dilution) in human chondrocytes (Cat no:20960-1-AP)

Diane (Verified Customer) (02-17-2021)

Separated on a 7.5% SDS PAGE gel, transferred to nitrocellulose membrane. Block with 5% dry milk/PBST at room temperature for one hour. 1:1000 dilution of the primary antibody in blocking solution. Incubated overnight at 4 degrees C on a rocker platform. Secondary antibody 1:1000 secondary (anti rabbit HRP) incubation for two hours at room temperature. Visualization with the Opti-4CN chromogenic substrate kit (BioRad).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: primary glioblastoma cell lysates
HIF-1 alpha Antibody Western Blot validation (1:1000 dilution) in primary glioblastoma cell lysates (Cat no:20960-1-AP)

Chun (Verified Customer) (12-05-2019)

A good antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: HeLa cells