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- KD/KO Validated
HINFP Polyclonal antibody
HINFP Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10066-2-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human liver tissue, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue |
Positive IF detected in | Hela cells, L02 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 1 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
10066-2-AP targets HINFP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HINFP fusion protein Ag0115 |
Full Name | histone H4 transcription factor |
Calculated molecular weight | 60 kDa |
Observed molecular weight | 60 kDa |
GenBank accession number | BC001073 |
Gene symbol | HINFP |
Gene ID (NCBI) | 25988 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Methylation of CpG dinucleotides is the major epigenetic modification in higher eukaryotic genomes. MIZF interacts with MBD2, a methyl-CpG-binding protein playing a critical role in DNA methylation-mediated transcriptional repression. MIZF functions as a negative regulator of transcription by binding to MBD2 and recruiting histone deacetylase (HDAC)-containing complexes. MIZF mRNA is present in various human tissues and cell lines and expressed as a 60kd protein.
Protocols
Product Specific Protocols | |
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WB protocol for HINFP antibody 10066-2-AP | Download protocol |
IF protocol for HINFP antibody 10066-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |