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HINFP Polyclonal antibody

HINFP Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10066-2-AP

Synonyms

HiNF P, HINFP, Histone nuclear factor P, MIZF, ZNF743



Tested Applications

Positive WB detected inHepG2 cells, human liver tissue, mouse liver tissue, rat liver tissue, L02 cells
Positive IP detected inL02 cells
Positive IF detected inHela cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10066-2-AP targets HINFP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HINFP fusion protein Ag0115
Full Name histone H4 transcription factor
Calculated molecular weight 60 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC001073
Gene symbol HINFP
Gene ID (NCBI) 25988
RRIDAB_2117412
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Methylation of CpG dinucleotides is the major epigenetic modification in higher eukaryotic genomes. MIZF interacts with MBD2, a methyl-CpG-binding protein playing a critical role in DNA methylation-mediated transcriptional repression. MIZF functions as a negative regulator of transcription by binding to MBD2 and recruiting histone deacetylase (HDAC)-containing complexes. MIZF mRNA is present in various human tissues and cell lines and expressed as a 60kd protein.

Protocols

Product Specific Protocols
WB protocol for HINFP antibody 10066-2-APDownload protocol
IF protocol for HINFP antibody 10066-2-APDownload protocol
IP protocol for HINFP antibody 10066-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Oncogene

Downregulation of HINFP induces senescence-associated secretory phenotype to promote metastasis in a non-cell-autonomous manner in bladder cancer.

Authors - Xianchong Zheng
  • KO Validated