HINFP Polyclonal antibody

• Featured Product
• KD/KO Validated

HINFP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10066-2-AP

Synonyms

MIZF, Methyl-CpG-binding protein 2-interacting zinc finger protein, MBD2-interacting zinc finger protein, Histone nuclear factor P, HiNF-P

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Validation Data Gallery

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  WB analysis using 10066-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10066-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 10066-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 10066-2-AP

  IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug.

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  IP experiment of L02 using 10066-2-AP

  IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug.

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  IF Staining of HeLa using 10066-2-AP

  Immunofluorescent analysis of fixed Hela cells using 10066-2-AP (HINFP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of L02 using 10066-2-AP

  Immunofluorescent analysis of L02 cells using 10066-2-AP (HINFP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, human liver tissue, mouse liver tissue, rat liver tissue, L02 cells
• Positive IP detected in: L02 cells
• Positive IF/ICC detected in: Hela cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10066-2-AP targets HINFP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HINFP fusion protein Ag0115
• Full Name: histone H4 transcription factor
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC001073
• Gene Symbol: HINFP
• Gene ID (NCBI): 25988
• RRID: AB_2117412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Methylation of CpG dinucleotides is the major epigenetic modification in higher eukaryotic genomes. MIZF interacts with MBD2, a methyl-CpG-binding protein playing a critical role in DNA methylation-mediated transcriptional repression. MIZF functions as a negative regulator of transcription by binding to MBD2 and recruiting histone deacetylase (HDAC)-containing complexes. MIZF mRNA is present in various human tissues and cell lines and expressed as a 60kd protein.

Publications

Species	Application	Title
human	WB,IHC,IF	Oncogene; Downregulation of HINFP induces senescence-associated secretory phenotype to promote metastasis in a non-cell-autonomous manner in bladder cancer.; Authors - Xianchong Zheng ; KO Validated