Acetyl-Histone H2A (Lys9) Recombinant antibody, PBS Only

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  WB analysis using 82823-2-RR (same clone as 82823-2-PBS)

  Trichostain A treated and untreated HeLa cell lysates were subjected to SDS PAGE followed by western blot with 82823-2-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 82823-2-PBS in a different storage buffer formulation.

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  Dot Blot experiment of peptide using 82823-2-RR (same clone as 82823-2-PBS)

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2A (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H2AK9ac. Column 2: Unmodified H2AK9. Column 3: H2AK5ac. Column 4: Unmodified H2AK5. Column 5: H2AK13Ac. Column 6: Unmodified H2AK13. Column 7: H2AK15ac. Column 8:Unmodified H2AK15. Column 9: Blank(PBS). This data was developed using the same antibody clone with 82823-2-PBS in a different storage buffer formulation.

Acetyl-Histone H2A (Lys9) Uni-rAb^TM Recombinant Antibody for WB, Dot Blot, Indirect ELISA

Cat No. 82823-2-PBS

Clone No.1O17

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Dot Blot, Indirect ELISA

Synonyms

Ac Histone H2A, Ac Histone H2A (Lys9), Ac Histone H2A Lys9, Ac Histone H2A-Lys9, Acetyl Histone

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Product Information

82823-2-PBS targets Acetyl-Histone H2A (Lys9) in WB, Dot Blot, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H2aa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC062211
• Gene Symbol: HIST1H2AA
• Gene ID (NCBI): 221613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96QV6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.