HK2 Monoclonal antibody

HK2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2A11C3

Cat no : 66974-1-Ig

Synonyms

DKFZp686M1669, hexokinase 2, Hexokinase type II, HK II, HK2, HKII, HXK2, Muscle form hexokinase



Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, RAW 264.7 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66974-1-Ig targets HK2 in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HK2 fusion protein Ag16895
Full Name hexokinase 2
Calculated molecular weight 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC021116
Gene symbol HK2
Gene ID (NCBI) 3099
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236)

Protocols

Product Specific Protocols
WB protocol for HK2 antibody 66974-1-IgDownload protocol
IHC protocol for HK2 antibody 66974-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway.

Authors - Yanqing Li
mouseWB

Acta Pharmacol Sin

Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis.

Authors - Lei Xu
humanWB

Evid Based Complement Alternat Med

Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma.

Authors - Ke Yang
mouseWB

Front Pharmacol

Bmal1 Regulates Macrophage Polarize Through Glycolytic Pathway in Alcoholic Liver Disease.

Authors - Yiwen Zhou
humanWB,IHC

Biochem Pharmacol

Ginsenoside Rh4 suppresses aerobic glycolysis and the expression of PD-L1 via targeting AKT in esophageal cancer.

Authors - Xuqian Deng