Hexokinase 2 Monoclonal antibody

Hexokinase 2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (4)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

28

CloneNo.

2A11C3

Cat no : 66974-1-Ig

Synonyms

DKFZp686M1669, hexokinase 2, Hexokinase type II, HK II, HK2, HKII, HXK2, Muscle form hexokinase

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Validation Data Gallery

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  WB analysis using 66974-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66974-1-Ig (Hexokinase 2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  IHC staining of mouse skeletal muscle using 66974-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66974-1-Ig (Hexokinase 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, 4T1 cells
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 26 publications below
• IHC: See 5 publications below

Product Information

66974-1-Ig targets Hexokinase 2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Hexokinase 2 fusion protein Ag16895
• Full Name: hexokinase 2
• Calculated molecular weight: 102 kDa
• Observed molecular weight: 102 kDa
• GenBank accession number: BC021116
• Gene symbol: HK2
• Gene ID (NCBI): 3099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236). The antibody also recognizes Hexokinase 1.

Publications

Species	Application	Title
mouse	WB	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
pig	WB	Emerg Microbes Infect; The regulation of cell homeostasis and antiviral innate immunity by autophagy during classical swine fever virus infection; Authors - Xiaowen Li
mouse	WB	Acta Pharmacol Sin; Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis.; Authors - Lei Xu
mouse	WB	Virol Sin; Integrated Metabolomics and Transcriptomics Analyses Reveal Metabolic Landscape in Neuronal Cells during JEV Infection.; Authors - Mengyuan Li
	WB	Food Chem; Phosphorylation and acetylation of glycolytic enzymes cooperatively regulate their activity and lamb meat quality; Authors - Chi Ren
human	WB	Int J Mol Sci; PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway.; Authors - Yanqing Li