Hexokinase 2 Monoclonal antibody
Hexokinase 2 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat and More (3)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2A11C3
Cat no : 66974-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, RAW 264.7 cells |
Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 11 publications below |
IHC | See 3 publications below |
Product Information
66974-1-Ig targets Hexokinase 2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Hexokinase 2 fusion protein Ag16895 |
Full Name | hexokinase 2 |
Calculated molecular weight | 102 kDa |
Observed molecular weight | 102 kDa |
GenBank accession number | BC021116 |
Gene symbol | HK2 |
Gene ID (NCBI) | 3099 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236)
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Hexokinase 2 antibody 66974-1-Ig | Download protocol |
IHC protocol for Hexokinase 2 antibody 66974-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Int J Mol Sci PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway. | ||
Acta Pharmacol Sin Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis. | ||
Virol Sin Integrated Metabolomics and Transcriptomics Analyses Reveal Metabolic Landscape in Neuronal Cells during JEV Infection. | ||
Evid Based Complement Alternat Med Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma. | ||
Am J Transl Res Myosin 1b promotes migration, invasion and glycolysis in cervical cancer via ERK/HIF-1α pathway. | ||
Front Pharmacol Bmal1 Regulates Macrophage Polarize Through Glycolytic Pathway in Alcoholic Liver Disease. |