Hexokinase 2 Monoclonal antibody

Hexokinase 2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A11C3

Cat no : 66974-1-Ig

Synonyms

DKFZp686M1669, hexokinase 2, Hexokinase type II, HK II, HK2, HKII, HXK2, Muscle form hexokinase



Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, 4T1 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66974-1-Ig targets Hexokinase 2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Hexokinase 2 fusion protein Ag16895
Full Name hexokinase 2
Calculated molecular weight 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC021116
Gene symbol HK2
Gene ID (NCBI) 3099
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236)

Protocols

Product Specific Protocols
WB protocol for Hexokinase 2 antibody 66974-1-IgDownload protocol
IHC protocol for Hexokinase 2 antibody 66974-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Food Chem

Phosphorylation and acetylation of glycolytic enzymes cooperatively regulate their activity and lamb meat quality

Authors - Chi Ren
humanIHC

Immunology

Ectopic endometriotic stromal cells-derived lactate induces M2 macrophage polarization via Mettl3/Trib1/ERK/STAT3 signaling pathway in endometriosis

Authors - Yanling Gou
humanWB,IHC

Biochem Pharmacol

Ginsenoside Rh4 suppresses aerobic glycolysis and the expression of PD-L1 via targeting AKT in esophageal cancer.

Authors - Xuqian Deng
humanWB

Biochem Pharmacol

RSL3 enhances the antitumor effect of cisplatin on prostate cancer cells via causing glycolysis dysfunction.

Authors - Mengxin Li
humanIHC

Front Oncol

MiR-489-3p Reduced Pancreatic Cancer Proliferation and Metastasis By Targeting PKM2 and LDHA Involving Glycolysis.

Authors - Dan Zhang
human,ratWB,IHC

Front Oncol

Activated Pancreatic Stellate Cells Enhance the Warburg Effect to Cause the Malignant Development in Chronic Pancreatitis.

Authors - Ye Tao