Hexokinase 2 Monoclonal antibody

Hexokinase 2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

19

CloneNo.

2A11C3

Cat no : 66974-1-Ig

Synonyms

DKFZp686M1669, hexokinase 2, Hexokinase type II, HK II, HK2, HKII, HXK2, Muscle form hexokinase

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Validation Data Gallery

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  WB analysis using 66974-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66974-1-Ig (Hexokinase 2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 66974-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66974-1-Ig (Hexokinase 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, 4T1 cells
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 17 publications below
• IHC: See 4 publications below

Product Information

66974-1-Ig targets Hexokinase 2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Hexokinase 2 fusion protein Ag16895
• Full Name: hexokinase 2
• Calculated molecular weight: 102 kDa
• Observed molecular weight: 102 kDa
• GenBank accession number: BC021116
• Gene symbol: HK2
• Gene ID (NCBI): 3099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236)

Publications

Species	Application	Title
	WB	Food Chem; Phosphorylation and acetylation of glycolytic enzymes cooperatively regulate their activity and lamb meat quality; Authors - Chi Ren
human	IHC	Immunology; Ectopic endometriotic stromal cells-derived lactate induces M2 macrophage polarization via Mettl3/Trib1/ERK/STAT3 signaling pathway in endometriosis; Authors - Yanling Gou
human	WB,IHC	Biochem Pharmacol; Ginsenoside Rh4 suppresses aerobic glycolysis and the expression of PD-L1 via targeting AKT in esophageal cancer.; Authors - Xuqian Deng
human	WB	Biochem Pharmacol; RSL3 enhances the antitumor effect of cisplatin on prostate cancer cells via causing glycolysis dysfunction.; Authors - Mengxin Li
human	IHC	Front Oncol; MiR-489-3p Reduced Pancreatic Cancer Proliferation and Metastasis By Targeting PKM2 and LDHA Involving Glycolysis.; Authors - Dan Zhang
human,rat	WB,IHC	Front Oncol; Activated Pancreatic Stellate Cells Enhance the Warburg Effect to Cause the Malignant Development in Chronic Pancreatitis.; Authors - Ye Tao