HNRNPU Polyclonal antibody

HNRNPU Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 14599-1-AP

Synonyms

hnRNP U, HNRNPU, HNRPU, p120, pp120, SAF A, SAFA, Scaffold attachment factor A, U21.1



Tested Applications

Positive WB detected inHEK-293 cells, mouse lung tissue, HeLa cells, K-562 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14599-1-AP targets HNRNPU in WB, RIP, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPU fusion protein Ag6141
Full Name heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
Calculated molecular weight 825 aa, 91 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC003621
Gene symbol HNRNPU
Gene ID (NCBI) 3192
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).

Protocols

Product Specific Protocols
WB protocol for HNRNPU antibody 14599-1-APDownload protocol
IF protocol for HNRNPU antibody 14599-1-APDownload protocol
IP protocol for HNRNPU antibody 14599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Nucleus

Myosin VI in the nucleus of neurosecretory PC12 Cells: Stimulation-dependent nuclear translocation and interaction with nuclear proteins.

Authors - Lukasz Majewski
mouseWB

Neurosci Lett

hnRNPM deficiency leads to cognitive deficits via disrupting synaptic plasticity.

Authors - Akinsola Raphael Akinyemi
WB

J Biol Chem

O-GlcNAcylation of Thr12 / Ser56 in short form O-GlcNAc transferase (sOGT) regulates its substrate selectivity.

Authors - Li Liu
mouseWB

iScience

Cdh1-APC Regulates Protein Synthesis and Stress Granules in Neurons through an FMRP-Dependent Mechanism.

Authors - Arielle N Valdez-Sinon
humanWB

Sci Rep

Identification of novel PANDAR protein interaction partners involved in splicing regulation.

Authors - N Pospiech
humanWB

Hum Mol Genet

Nuclear TDP-43 causes neuronal toxicity by escaping from the inhibitory regulation by hnRNPs.

Authors - Hiroaki Suzuki