HNRNPU Polyclonal antibody

• Home
• Products
• Primary Antibodies
• HNRNPU Polyclonal antibody (14599-1-AP)

HNRNPU Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 14599-1-AP Copied!

Publications (34)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Synonyms

C1orf199, GRIP120, hnRNP U, HNRPU, Nuclear p120 ribonucleoprotein

• 

  WB analysis using 14599-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14599-1-AP (HNRNPU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse lung using 14599-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14599-1-AP (HNRNPU antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 14599-1-AP

  IP result of anti-HNRNPU (IP:14599-1-AP, 4ug; Detection:14599-1-AP 1:800) with HeLa cells lysate 2400ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 14599-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPU antibody (14599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for HNRNPU in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HEK-293 cells, mouse lung tissue, HeLa cells, Jurkat cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14599-1-AP targets HNRNPU in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6141

  Product name: Recombinant human HNRNPU protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 10-236 aa of BC003621

  Sequence: KLKVSELKEELKKRRLSDKGLKAELMERLQAALDDEEAGGRPAMEPGNGSLDLGGDSAGRSGAGLEQEAAAGGDEEEEEEEEEEEGISALDGDQMELGEENGAAGAADSGPMEEEEAASEDENGDDQGFQEGEDELGDEEEGAGDENGHGEQQPQPPATQQQQPQQQRGAAKEAAGKSSGPTSLFAVTVAPPGARQGQQQAGGDGKTEQKGGDKKRGVKRPREDHGR

• Full Name: heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
• Calculated Molecular Weight: 825 aa, 91 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC003621
• Gene Symbol: HNRNPU
• Gene ID (NCBI): 3192
• RRID: AB_2248577
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q00839
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).

Publications

What published studies show

The HNRNPU Polyclonal antibody (Cat# 14599-1-AP) has 34 citations published in journals including Nature, Cell, Nature Cell Biology, Nature Communications, Cancer Research, Nucleic Acids Research, Oncogene, and Advanced Science. Research fields span RNA biology and alternative splicing, multiple cancer types (gastric, cervical, colorectal, lung, breast, prostate, RCC), viral infections, inflammation, pulmonary fibrosis, neurodegeneration, diabetic nephropathy, and osteoarthritis.

Automatically generated from the 34 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Nature; GlycoRNA complexed with heparan sulfate regulates VEGF-A signalling.; Authors - Peiyuan Chai
human	WB,IF	Cell; RNA-binding proteins and glycoRNAs form domains on the cell surface for cell-penetrating peptide entry; Authors - Jonathan Perr
human	WB	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
human	WB	Nat Aging; Aging-associated lncRNAs are evolutionarily conserved and participate in NFκB signaling; Authors - Donghong Cai
human	WB,RIP,IF,CoIP	Nat Cell Biol; The nuclear matrix stabilizes primed-specific genes in human pluripotent stem cells; Authors - Gang Ma
human	WB,RIP	Cancer Res; DIS3L2 promotes progression of hepatocellular carcinoma via hnRNP U-mediated alternative splicing.; Authors - Songge Xing