|Positive WB detected in||HEK-293 cells, mouse lung tissue, HeLa cells, K-562 cells|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14599-1-AP targets HNRNPU in WB, RIP, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HNRNPU fusion protein Ag6141|
|Full Name||heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)|
|Calculated molecular weight||825 aa, 91 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC003621|
|Gene ID (NCBI)||3192|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).
Myosin VI in the nucleus of neurosecretory PC12 Cells: Stimulation-dependent nuclear translocation and interaction with nuclear proteins.
hnRNPM deficiency leads to cognitive deficits via disrupting synaptic plasticity.
J Biol Chem
O-GlcNAcylation of Thr12 / Ser56 in short form O-GlcNAc transferase (sOGT) regulates its substrate selectivity.
Cdh1-APC Regulates Protein Synthesis and Stress Granules in Neurons through an FMRP-Dependent Mechanism.
Identification of novel PANDAR protein interaction partners involved in splicing regulation.
Hum Mol Genet
Nuclear TDP-43 causes neuronal toxicity by escaping from the inhibitory regulation by hnRNPs.