HOXB5 Polyclonal antibody

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  WB analysis using 31552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31552-1-AP (HOXB5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HOXB5 Polyclonal Antibody for WB, ELISA

Cat No. 31552-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

HU 1, HOX2A, HHO.C10

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse epididymis tissue, rat colon

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31552-1-AP targets HOXB5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOXB5 fusion protein Ag35933
• Full Name: homeobox B5
• Calculated Molecular Weight: 29kDa，269aa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: NM_002147.3
• Gene Symbol: HOXB5
• Gene ID (NCBI): 3215
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P09067
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOXB5 is a member of the Antp homeobox family. HOXB5 is expressed in various tissues, with biased expression in the kidney, colon, and other tissues. HOXB5 plays a crucial role in the development of the lung and gut. It is essential for the proper formation and differentiation of these organs during embryonic development. Hoxb5 exhibits preferential expression in hematopoietic stem cells (HSCs) and uniquely marks the long-term HSCs (LT-HSCs) (PMID: 38324932).