HPSE2 Polyclonal antibody

• 

  WB analysis of L02 using 21128-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21128-1-AP (HPSE2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21128-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21128-1-AP (HPSE2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

HPSE2 Polyclonal Antibody for WB, ELISA

Cat No. 21128-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Synonyms

heparanase 2, Hpa2, HPR2, Inactive heparanase-2

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Tested Applications

• Positive WB detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

21128-1-AP targets HPSE2 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HPSE2 fusion protein Ag15526
• Full Name: heparanase 2
• Calculated Molecular Weight: 592 aa, 67 kDa
• Observed Molecular Weight: 54 kDa, 66 kDa
• GenBank Accession Number: BC112356
• Gene Symbol: HPSE2
• Gene ID (NCBI): 60495
• RRID: AB_10733344
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WWQ2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HPSE2 (heparanase 2) is a homolog of HPSE (heparanase 1). HPSE is an endo-β-glucuronidase that cleaves heparan sulfate proteoglycans (HSPGs), which are important components of the extracellular matrix and basement membranes. HPSE2, however, lacks intrinsic heparan sulfate-degrading activity. Instead, it binds to heparan sulfate with higher affinity than HPSE, thereby competing with HPSE for heparan sulfate binding and inhibiting HPSE's enzymatic activity. This competitive inhibition makes HPSE2 a crucial regulator of heparan sulfate metabolism.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Viruses; Heparanase 2 Modulation Inhibits HSV-2 Replication by Regulating Heparan Sulfate; Authors - James Hopkins