Validation Data Gallery
|Positive WB detected in||HeLa cells, Recombinant protein protein, HEK-293 cells, whole yeast cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:40000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
66183-1-Ig targets HSP70 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, yeast, mouse, rat samples.
|Tested Reactivity||human, yeast, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||HSP70 fusion protein Ag19111|
|Full Name||heat shock 70kDa protein 1A|
|Calculated molecular weight||70 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC009322|
|Gene ID (NCBI)||3303|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker. This antibody well recognized the endogenous HSP70 protein in multiple cell lines. (21373891)
|Product Specific Protocols|
|WB protocol for HSP70 antibody 66183-1-Ig||Download protocol|
|IHC protocol for HSP70 antibody 66183-1-Ig||Download protocol|
|IF protocol for HSP70 antibody 66183-1-Ig||Download protocol|
|FC protocol for HSP70 antibody 66183-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Daniela (Verified Customer) (10-04-2022)
Very good specific antibody, no detection of no-specific bands in HeLa cell lysate. Two close bands at two different intensities are consistently detected below 75KDa in HeLa cell lysate.