HSP70 Monoclonal antibody

HSP70 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, yeast, mouse, rat

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

4E7E5

Cat no : 66183-1-Ig

Synonyms

Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B



Tested Applications

Positive WB detected inHeLa cells, Recombinant protein protein, HEK-293 cells, whole yeast cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:40000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

66183-1-Ig targets HSP70 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, yeast, mouse, rat samples.

Tested Reactivity human, yeast, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSP70 fusion protein Ag19111
Full Name heat shock 70kDa protein 1A
Calculated molecular weight 70 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC009322
Gene symbol HSPA1A
Gene ID (NCBI) 3303
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker. This antibody well recognized the endogenous HSP70 protein in multiple cell lines. (21373891)

Protocols

Product Specific Protocols
WB protocol for HSP70 antibody 66183-1-IgDownload protocol
IHC protocol for HSP70 antibody 66183-1-IgDownload protocol
IF protocol for HSP70 antibody 66183-1-IgDownload protocol
FC protocol for HSP70 antibody 66183-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Commun

Autophagy-mediated apoptosis eliminates aneuploid cells in a mouse model of chromosome mosaicism.

Authors - Shruti Singla
ratWB

Bioact Mater

Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.

Authors - Liwen Luo
humanWB

Cells

Proteomics, Phosphoproteomics and Mirna Analysis of Circulating Extracellular Vesicles through Automated and High-Throughput Isolation.

Authors - Hao Zhang
humanWB

Mol Ther Oncolytics

Exosomal miR-205-5p enhances angiogenesis and nasopharyngeal carcinoma metastasis by targeting desmocollin-2.

Authors - Wenjuan Yang
mouse,humanIHC,CoIP

Genes (Basel)

BAG3 Alleviates Atherosclerosis by Inhibiting Endothelial-to-Mesenchymal Transition via Autophagy Activation.

Authors - Hongtao Diao
humanWB

Cell Mol Biol Lett

Serum exosomal pregnancy zone protein as a promising biomarker in inflammatory bowel disease.

Authors - Jing Shao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Daniela (Verified Customer) (10-04-2022)

Very good specific antibody, no detection of no-specific bands in HeLa cell lysate. Two close bands at two different intensities are consistently detected below 75KDa in HeLa cell lysate.

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells
HSP70 Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in HeLa cells (Cat no:66183-1-Ig)