HSP90 Monoclonal antibody
HSP90 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 60318-1-Ig
|Positive WB detected in||LNCaP cells, HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, ROS1728 cells, NIH/3T3 cells, 4T1 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||K-562 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
60318-1-Ig targets HSP90 in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse, rat, monkey, pig|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||HSP90 fusion protein Ag3826|
|Full Name||heat shock protein 90kDa alpha (cytosolic), class A member 1|
|Calculated molecular weight||853 aa, 90 kDa|
|Observed molecular weight||85-90 kDa|
|GenBank accession number||BC023006|
|Gene ID (NCBI)||3320|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.
|Product Specific Protocols|
|WB protocol for HSP90 antibody 60318-1-Ig||Download protocol|
|IHC protocol for HSP90 antibody 60318-1-Ig||Download protocol|
|IF protocol for HSP90 antibody 60318-1-Ig||Download protocol|
|IP protocol for HSP90 antibody 60318-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (10-05-2021)
This antibody recognized a strong band around 90 kd, as shown in the website.
Ayyappan (Verified Customer) (01-16-2019)
This antibody works well for IP in human cancer cell lines