HSP90 Monoclonal antibody

HSP90 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

3F11C1

Cat no : 60318-1-Ig

Synonyms

Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2



Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, ROS1728 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60318-1-Ig targets HSP90 in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, monkey, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HSP90 fusion protein Ag3826
Full Name heat shock protein 90kDa alpha (cytosolic), class A member 1
Calculated molecular weight 853 aa, 90 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC023006
Gene symbol HSP90AA1
Gene ID (NCBI) 3320
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.

Protocols

Product Specific Protocols
WB protocol for HSP90 antibody 60318-1-IgDownload protocol
IHC protocol for HSP90 antibody 60318-1-IgDownload protocol
IF protocol for HSP90 antibody 60318-1-IgDownload protocol
IP protocol for HSP90 antibody 60318-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Sci Adv

YAP drives fate conversion and chemoresistance of small cell lung cancer.

Authors - Qingzhe Wu
humanWB,IP,RIP

Mol Cancer

Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation.

Authors - Mengyan Xie
human,mouseWB,IF

Redox Biol

Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.

Authors - Zhongren Xu
mouseWB,IP,CoIP

Cell Rep

The LIS1/NDE1 Complex Is Essential for FGF Signaling by Regulating FGF Receptor Intracellular Trafficking.

Authors - Liansheng Liu
humanWB

Elife

MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.

Authors - Yuichiro Saito
humanWB

BMC Med

NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma

Authors - Yizhi Zhan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (10-05-2021)

This antibody recognized a strong band around 90 kd, as shown in the website.

  • Applications: Western Blot
FH

Ayyappan (Verified Customer) (01-16-2019)

This antibody works well for IP in human cancer cell lines

  • Applications: Immunoprecipitation,