Validation Data Gallery
|Positive WB detected in||human ileum tissue, A431 cells, HeLa cells, human brain tissue, human kidney tissue, human skeletal muscle tissue, MCF-7 cells|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human testis tissue, human prostate hyperplasia tissue, human ovary tumor tissue, mouse ovary tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12797-1-AP targets HSPA2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HSPA2 fusion protein Ag3539|
|Full Name||heat shock 70kDa protein 2|
|Calculated molecular weight||639 aa, 70 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC036107|
|Gene ID (NCBI)||3306|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)
Molecular analysis of cell type-specific gene expression profile during mouse spermatogenesis by laser microdissection and qRT-PCR.
Mol Hum Reprod
Melatonin protects the mouse testis against heat-induced damage.
Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.
Int J Mol Sci
Various Anti-HSPA2 Antibodies Yield Different Results in Studies on Cancer-Related Functions of Heat Shock Protein A2.
Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes.
J Mol Cell Biol
Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through cross talk between Ahr-Nrf2 pathway and MAPK signaling.