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HSPA2 Polyclonal antibody

HSPA2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12797-1-AP


Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2

Tested Applications

Positive WB detected inA431 cells, human brain tissue, human kidney tissue, human skeletal muscle tissue, HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, C6 cells, mouse skeletal tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman testis tissue, human prostate hyperplasia tissue, human ovary tumor tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12797-1-AP targets HSPA2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSPA2 fusion protein Ag3539
Full Name heat shock 70kDa protein 2
Calculated Molecular Weight 639 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC036107
Gene Symbol HSPA2
Gene ID (NCBI) 3306
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)


Product Specific Protocols
WB protocol for HSPA2 antibody 12797-1-APDownload protocol
IHC protocol for HSPA2 antibody 12797-1-APDownload protocol
IP protocol for HSPA2 antibody 12797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Adv

Circular RNAs from BOULE play conserved roles in protection against stress-induced fertility decline.

Authors - Liuze Gao

Cell Death Differ

RNF144A functions as a tumor suppressor in breast cancer through ubiquitin ligase activity-dependent regulation of stability and oncogenic functions of HSPA2.

Authors - Yin-Long Yang
  • KD Validated

Curr Biol

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Authors - Long Jiang

J Mol Cell Biol

Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through cross talk between Ahr-Nrf2 pathway and MAPK signaling.

Authors - Prabagaran Esakky


Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes.

Authors - Sun Fengyun F
humanWB, IHC

Int J Mol Sci

Various Anti-HSPA2 Antibodies Yield Different Results in Studies on Cancer-Related Functions of Heat Shock Protein A2.

Authors - Dorota Scieglinska
  • KD Validated