HSPA2 Polyclonal antibody

HSPA2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12797-1-AP

Synonyms

Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2



Tested Applications

Positive WB detected inhuman ileum tissue, A431 cells, HeLa cells, human brain tissue, human kidney tissue, human skeletal muscle tissue, MCF-7 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman testis tissue, human prostate hyperplasia tissue, human ovary tumor tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12797-1-AP targets HSPA2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSPA2 fusion protein Ag3539
Full Name heat shock 70kDa protein 2
Calculated molecular weight 639 aa, 70 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC036107
Gene symbol HSPA2
Gene ID (NCBI) 3306
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)

Protocols

Product Specific Protocols
WB protocol for HSPA2 antibody 12797-1-APDownload protocol
IHC protocol for HSPA2 antibody 12797-1-APDownload protocol
IP protocol for HSPA2 antibody 12797-1-APDownload protocol
FC protocol for HSPA2 antibody 12797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB, IHC

Reprod Sci

Molecular analysis of cell type-specific gene expression profile during mouse spermatogenesis by laser microdissection and qRT-PCR.

Authors - Esakky Prabagaran P
mouseWB,IHC

Mol Hum Reprod

Melatonin protects the mouse testis against heat-induced damage.

Authors - Pengfei Zhang
mouseIF

Biol Reprod

Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.

Authors - Jianjun Hu
humanWB, IHC

Int J Mol Sci

Various Anti-HSPA2 Antibodies Yield Different Results in Studies on Cancer-Related Functions of Heat Shock Protein A2.

Authors - Dorota Scieglinska
  • KD Validated
mouseWB,IHC

Development

Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes.

Authors - Sun Fengyun F

J Mol Cell Biol

Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through cross talk between Ahr-Nrf2 pathway and MAPK signaling.

Authors - Prabagaran Esakky