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HSPA2 Polyclonal antibody
HSPA2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, FC, ELISA
Cat no : 12797-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, human brain tissue, human kidney tissue, human skeletal muscle tissue, HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, C6 cells, mouse skeletal tissue|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human testis tissue, human prostate hyperplasia tissue, human ovary tumor tissue, mouse ovary tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 3 publications below|
|WB||See 7 publications below|
|IHC||See 6 publications below|
|IF||See 2 publications below|
|IP||See 1 publications below|
12797-1-AP targets HSPA2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HSPA2 fusion protein Ag3539|
|Full Name||heat shock 70kDa protein 2|
|Calculated molecular weight||639 aa, 70 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC036107|
|Gene ID (NCBI)||3306|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)
|Product Specific Protocols|
|WB protocol for HSPA2 antibody 12797-1-AP||Download protocol|
|IHC protocol for HSPA2 antibody 12797-1-AP||Download protocol|
|IP protocol for HSPA2 antibody 12797-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Circular RNAs from BOULE play conserved roles in protection against stress-induced fertility decline.
Cell Death Differ
RNF144A functions as a tumor suppressor in breast cancer through ubiquitin ligase activity-dependent regulation of stability and oncogenic functions of HSPA2.
RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.
J Mol Cell Biol
Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through cross talk between Ahr-Nrf2 pathway and MAPK signaling.
Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes.
Int J Mol Sci
Various Anti-HSPA2 Antibodies Yield Different Results in Studies on Cancer-Related Functions of Heat Shock Protein A2.