HSP27 Polyclonal antibody

• Featured Product
• KD/KO Validated

HSP27 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

31

Reviews (1)

Cat no : 18284-1-AP

Synonyms

Heat shock protein beta 1, HSP 27, Hsp25, HSP27, HSP28, HSPB1

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Validation Data Gallery

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  WB analysis using 18284-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18284-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18284-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 18284-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18284-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 18284-1-AP (HSP27 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 18284-1-AP

  Immunofluorescent analysis of MCF-7 cells, using HSPB1 antibody 18284-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 18284-1-AP

  Immunofluorescent analysis of Hela cells, using HSPB1 antibody 18284-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of MCF-7 using 18284-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human HSP27 (18284-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: A549 cells, C6 cells, mouse liver tissue, HeLa cells, HepG2 cells, U2OS cells
• Positive IHC detected in: human lung cancer tissue, human liver cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, MCF-7 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 23 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Product Information

18284-1-AP targets HSP27 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse, rat, fish, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSP27 fusion protein Ag13161
• Full Name: heat shock 27kDa protein 1
• Calculated molecular weight: 19-23 kDa
• Observed molecular weight: 27 kDa
• GenBank accession number: BC012768
• Gene symbol: HSPB1
• Gene ID (NCBI): 3315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPB1, also known as heat shock protein 27 (HSP27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (PMID: 21353161). It's notable that mouse HSP27 is also named HSP25 and has the predicted MW between 19-23 kDa.

Publications

Species	Application	Title
		Autophagy; ATG4B/autophagin-1 regulates intestinal homeostasis and protects mice from experimental colitis.; Authors - Cabrera Sandra S
rat	IF	J Cell Biol; Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins.; Authors - Sebastian Kötter
human	WB	Antioxid Redox Signal; Peroxiredoxin 6 is a crucial factor in the initial step of mitochondrial clearance and is upstream of the PINK1-Parkin pathway.; Authors - Shuaipeng Ma
human	WB,IHC	CNS Neurosci Ther; FHOD1 is upregulated in glioma cells and attenuates ferroptosis of glioma cells by targeting HSPB1 signaling; Authors - Fan Zhang
fish	ELISA	Food Res Int; Inhibitory effect of edible natural compounds with di- and tri-carboxyl moiety on endogenous protease inducing disassembly and degradation of myofibrils from grass carp (Ctenopharyngodon idella).; Authors - Lihong Ge
human	WB	Biomedicines; c-Myc Sustains Pancreatic Cancer Cell Survival and mutp53 Stability through the Mevalonate Pathway; Authors - Maria Anele Romeo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (09-28-2020)

HEK293T lysate (10ug) ran on 4-20% gradient gel. Blocking in 5% BSA for 1 hour, followed by HSP27 (1:1000 in block) overnight at 4 degrees. Goat anti-rabbit HRP (1:10,000) for 1 hour in block.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

HSP27 Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:18284-1-AP)

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