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HSP27 Polyclonal antibody
HSP27 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse and More (2)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 18284-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, C6 cells, mouse liver tissue, HeLa cells, HepG2 cells, U2OS cells |
Positive IHC detected in | human lung cancer tissue, human liver cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, MCF-7 cells |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 23 publications below |
IHC | See 4 publications below |
IF | See 4 publications below |
IP | See 1 publications below |
ELISA | See 1 publications below |
Product Information
18284-1-AP targets HSP27 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, mouse, rat, fish, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HSP27 fusion protein Ag13161 |
Full Name | heat shock 27kDa protein 1 |
Calculated molecular weight | 19-23 kDa |
Observed molecular weight | 27 kDa |
GenBank accession number | BC012768 |
Gene symbol | HSPB1 |
Gene ID (NCBI) | 3315 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HSPB1, also known as heat shock protein 27 (HSP27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (PMID: 21353161). It's notable that mouse HSP27 is also named HSP25 and has the predicted MW between 19-23 kDa.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HSP27 antibody 18284-1-AP | Download protocol |
IHC protocol for HSP27 antibody 18284-1-AP | Download protocol |
IF protocol for HSP27 antibody 18284-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Autophagy ATG4B/autophagin-1 regulates intestinal homeostasis and protects mice from experimental colitis. | ||
J Cell Biol Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins. | ||
Antioxid Redox Signal Peroxiredoxin 6 is a crucial factor in the initial step of mitochondrial clearance and is upstream of the PINK1-Parkin pathway. | ||
CNS Neurosci Ther FHOD1 is upregulated in glioma cells and attenuates ferroptosis of glioma cells by targeting HSPB1 signaling | ||
Food Res Int Inhibitory effect of edible natural compounds with di- and tri-carboxyl moiety on endogenous protease inducing disassembly and degradation of myofibrils from grass carp (Ctenopharyngodon idella). | ||
Biomedicines c-Myc Sustains Pancreatic Cancer Cell Survival and mutp53 Stability through the Mevalonate Pathway |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tom (Verified Customer) (09-28-2020) | HEK293T lysate (10ug) ran on 4-20% gradient gel. Blocking in 5% BSA for 1 hour, followed by HSP27 (1:1000 in block) overnight at 4 degrees. Goat anti-rabbit HRP (1:10,000) for 1 hour in block.
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