|Positive WB detected in||HEK-293 cells, COS-7 cells, HeLa cells, human heart tissue, Jurkat cells, MCF-7 cells, mouse skeletal muscle tissue, Raji cells|
|Positive IP detected in||Raji cells|
|Positive IHC detected in||human kidney tissue, human colon tissue, human thyroid tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
15775-1-AP targets HTRA2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
|Tested Reactivity||human, mouse, rat, monkey|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HTRA2 fusion protein Ag8455|
|Full Name||HtrA serine peptidase 2|
|Calculated molecular weight||49 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC000096|
|Gene ID (NCBI)||27429|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HTRA2(High temperature requirement protein A2) is also named as OMI, PRSS25 and belongs to the peptidase S1B family. HTRA2 normally exists in the mitochondria, can cause MMP3 activation in the cytosol under a cell stress condition, which can ultimately lead to demise of dopaminergic neuronal cells(PMID: 22265821). It inhibits mitochondrial superoxide generation, stabilizes mitochondrial membrane potential, and prevents apoptosis at baseline and in response to extracellular inducers of mitochondrial stress(PMID: 18772386). This protein has 4 isoforms produced by alternative splicing.
Protective effects of ghrelin on brain mitochondria after cardiac arrest and resuscitation.
Mol Med Rep
Mechanisms underlying the protective effect of tannic acid against arsenic trioxide‑induced cardiotoxicity in rats: Potential involvement of mitochondrial apoptosis.
Association of Omi/HtrA2 with γ-secretase in mitochondria.
Global Interactome Mapping of Mitochondrial Intermembrane Space Proteases Identifies a Novel Function for HTRA2.
Establishment of MAGEC2-knockout cells and functional investigation of MAGEC2 in tumor cells.
Hum Mol Genet
Multi-OMICS study of a CHCHD10 variant causing ALS demonstrates metabolic rewiring and activation of endoplasmic reticulum and mitochondrial unfolded protein responses.