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  • KD/KO Validated

HTRA2 Polyclonal antibody

HTRA2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15775-1-AP

Synonyms

HtrA serine peptidase 2, HTRA2, OMI, PARK13, PRSS25, Serine protease 25, Serine proteinase OMI



Tested Applications

Positive WB detected inHEK-293 cells, COS-7 cells, HeLa cells, human heart tissue, Jurkat cells, MCF-7 cells, mouse skeletal muscle tissue, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman kidney tissue, human colon tissue, human thyroid tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15775-1-AP targets HTRA2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HTRA2 fusion protein Ag8455
Full Name HtrA serine peptidase 2
Calculated molecular weight 49 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000096
Gene symbol HTRA2
Gene ID (NCBI) 27429
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HTRA2(High temperature requirement protein A2) is also named as OMI, PRSS25 and belongs to the peptidase S1B family. HTRA2 normally exists in the mitochondria, can cause MMP3 activation in the cytosol under a cell stress condition, which can ultimately lead to demise of dopaminergic neuronal cells(PMID: 22265821). It inhibits mitochondrial superoxide generation, stabilizes mitochondrial membrane potential, and prevents apoptosis at baseline and in response to extracellular inducers of mitochondrial stress(PMID: 18772386). This protein has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for HTRA2 antibody 15775-1-APDownload protocol
IHC protocol for HTRA2 antibody 15775-1-APDownload protocol
IP protocol for HTRA2 antibody 15775-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

Neuropeptides

Protective effects of ghrelin on brain mitochondria after cardiac arrest and resuscitation.

Authors - Hongying Xu
ratIHC

Mol Med Rep

Mechanisms underlying the protective effect of tannic acid against arsenic trioxide‑induced cardiotoxicity in rats: Potential involvement of mitochondrial apoptosis.

Authors - Yucong Xue
human,mouseWB,IF

Neurochem Int

Association of Omi/HtrA2 with γ-secretase in mitochondria.

Authors - Behbahani Homira H
humanWB

Proteomics

Global Interactome Mapping of Mitochondrial Intermembrane Space Proteases Identifies a Novel Function for HTRA2.

Authors - Aaron Botham
  • KD Validated
humanWB

Cancer Sci

Establishment of MAGEC2-knockout cells and functional investigation of MAGEC2 in tumor cells.

Authors - Jingjing Wang
humanWB

Hum Mol Genet

Multi-OMICS study of a CHCHD10 variant causing ALS demonstrates metabolic rewiring and activation of endoplasmic reticulum and mitochondrial unfolded protein responses.

Authors - Isabella R Straub