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  • KD/KO Validated

HTRA2 Polyclonal antibody

HTRA2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, monkey and More (1)





Cat no : 15775-1-AP


HtrA serine peptidase 2, HTRA2, OMI, PARK13, PRSS25, Serine protease 25, Serine proteinase OMI

Tested Applications

Positive WB detected inHeLa cells, human heart tissue, mouse skeletal muscle tissue, Raji cells, MCF-7 cells, Jurkat cells, HEK-293 cells, SH-SY5Y cells, COS-7 cells, Neuro-2a cells
Positive IP detected inRaji cells
Positive IHC detected inhuman kidney tissue, human colon tissue, human thyroid tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15775-1-AP targets HTRA2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HTRA2 fusion protein Ag8455
Full Name HtrA serine peptidase 2
Calculated molecular weight 49 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000096
Gene symbol HTRA2
Gene ID (NCBI) 27429
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HTRA2(High temperature requirement protein A2) is also named as OMI, PRSS25 and belongs to the peptidase S1B family. HTRA2 normally exists in the mitochondria, can cause MMP3 activation in the cytosol under a cell stress condition, which can ultimately lead to demise of dopaminergic neuronal cells(PMID: 22265821). It inhibits mitochondrial superoxide generation, stabilizes mitochondrial membrane potential, and prevents apoptosis at baseline and in response to extracellular inducers of mitochondrial stress(PMID: 18772386). This protein has 4 isoforms produced by alternative splicing.


Product Specific Protocols
WB protocol for HTRA2 antibody 15775-1-APDownload protocol
IHC protocol for HTRA2 antibody 15775-1-APDownload protocol
IP protocol for HTRA2 antibody 15775-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Neurobiol

Mitochondrial Proteome Changes Correlating with β-Amyloid Accumulation.

Authors - Katalin Völgyi

Hum Mol Genet

Multi-OMICS study of a CHCHD10 variant causing ALS demonstrates metabolic rewiring and activation of endoplasmic reticulum and mitochondrial unfolded protein responses.

Authors - Isabella R Straub

Cancer Sci

Establishment of MAGEC2-knockout cells and functional investigation of MAGEC2 in tumor cells.

Authors - Jingjing Wang

Cancer Biomark

Role of Smac, survivin, XIAP, and Omi/HtrA2 proteins in determining the chemotherapeutic response of patients with cervical cancer treated with neoadjuvant chemotherapy

Authors - Tianyi Ye


Global Interactome Mapping of Mitochondrial Intermembrane Space Proteases Identifies a Novel Function for HTRA2.

Authors - Aaron Botham
  • KD Validated

Neurochem Int

Association of Omi/HtrA2 with γ-secretase in mitochondria.

Authors - Behbahani Homira H