Histone H3 antibody (mAb) (Clone MABI 0301)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Wide Range Predicted

Applications

ChIP, ChIP-Seq, ICC, IF, WB

CloneNo.

MABI 0301

Cat No : 39064,39763

Synonyms

histone H3, histone, H3, histone-H3, histoneH3, c-terminal, pAb, polyclonal, antibody, antibodies, chip, chromatin immunoprecipitation, 61317

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Validation Data Gallery

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  Histone H3 antibody (mAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from Farage human lymph-node derived B cells and 4 ug Histone H3 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 17.7 million sequence tags were mapped to identify Histone H3 binding sites.

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  Histone H3 antibody (mAb) tested by ChIP. ChIP performed on Active Motif Ready-to-ChIP HeLa cell chromatin (Catalog No. 53015) using Histone H3 antibody (mAb). PCR was performed using primers specific for the promoter region of the human GAPDH gene. Lane 1: ChIP using mouse IgG as a negative control. Lane 2: ChIP using 10 ug of Histone H3 antibody (mAb). Lane 3: Input DNA control.

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  Histone H3 antibody (mAb) tested by immunofluorescence. HeLa cells stained with Histone H3 antibody (mAb) at a dilution of 1 ug/ml.

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  Histone H3 antibody (mAb) tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with Histone H3 antibody (mab) at a dilution of 2 ug/ml. Lane 1: Nuclear extract of untreated HeLa cells. Lane 2: Nuclear extract of HeLa cells treated with sodium butyrate.

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Product Information

• Tested Applications: ChIP, ChIP-Seq, ICC, IF, WB

  Applications Validated by Active Motif: ChIP-Seq: 4 ug per ChIP ChIP: 5 - 10 ug per ChIP ICC/IF: 1 ug/ml dilution WB: 0.5 - 2 ug/ml dilution For Histone H3, we also offer AbFlex Histone H3 Recombinant Antibody (rAb). For details, see Catalog No. 91297.

• Tested Reactivity: Human, Wide Range Predicted
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: This Histone H3 antibody (mAb) was raised against a peptide containing the N-terminus of histone H3.
• Full Name: Histone H3 antibody (mAb) (Clone MABI 0301)
• Synonyms: histone H3, histone, H3, histone-H3, histoneH3, c-terminal, pAb, polyclonal, antibody, antibodies, chip, chromatin immunoprecipitation, 61317
• Molecular weight: 17 kDa
• GenBank accession number: NP_003522
• RRID: AB_2650522
• Purification Method: Protein G Chromatography
• Buffer: PBS pH 7.5 containing 30% glycerol. 0.3M NaCl, and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Each histone contains two domains. First, a main globular domain (C-terminal) forming the core of the nucleosome is involved in histone-histone interactions and in binding to the DNA. Secondly, an N-terminal tail is subject to post-translational modifications. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.