Histone H3K14ac antibody (mAb) (Clone 13HH3-1A5)
Host / Isotype
Mouse / IgG1, k
Reactivity
Human, Mouse, Wide Range Predicted
Applications
ChIP, ChIP-Seq, WB
CloneNo.
13HH3-1A5
Cat No : 61433 61433
Synonyms
Validation Data Gallery
Product Information
| Tested Applications | ChIP, ChIP-Seq, WB |
| Tested Reactivity | Human, Mouse, Wide Range Predicted |
| Host / Isotype | Mouse / IgG1, k |
| Class | Monoclonal |
| Type | Antibody |
| Modification | Acetylated |
| Immunogen | This antibody was raised against a peptide corresponding to amino acids 10-18 including acetyl-lysine 14 of human Histone H3. |
| Full Name | Histone H3K14ac antibody (mAb) (Clone 13HH3-1A5) |
| Synonyms | histone H3, histone, H3, histone-H3, histoneH3, mAb, monoclonal, H3 AcK14, Histone H3 Acetyl Lys14, Histone H3 Acetyl K14, Histone H3 Ac Lys14, H3 Acetyl Lys14, H3 Acetyl K14, H3 Ac Lys14, Histone H3 Acetylated Lysine 14, Histone H3 Acetyl Lysine14, H3 Acetylated Lysine 14, H3 Acetyl Lysine14, antibody, antibodies, H3K14Ac, chromatin immunoprecipitation, chip |
| Molecular weight | 17 kDa |
| GenBank accession number | NP_003522 |
| RRID | AB_2793634 |
| Purification Method | Protein A Chromatography |
| Buffer | Purified IgG in 60 mM Sodium Citrate, 105 mM Tris-HCl, 30% glycerol, and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization.