Histone H3K14me2 antibody (pAb)

Host / Isotype

Rabbit / Serum

Reactivity

Human, Wide Range Predicted

Applications

WB

Cat No : 39349,39350

Synonyms

histone H3, histone, H3, histone-H3, histoneH3, pAb, polyclonal, H3K14me2, Histone H3 dimethyl Lys14, Histone H3 dimethyl Lysine 14, Histone H3 dimethyl Lys 14, Histone H3 dimethyl K14, Histone H3 dimethyl K 14, Histone H3 di-methyl Lys14, Histone H3 di-methyl Lysine 14, Histone H3 di-methyl Lys 14, Histone H3 di-methyl K14, Histone H3 di-methyl K 14, Histone H3 2Me Lys14, Histone H3 2Me Lysine 14, Histone H3 2Me Lys 14, Histone H3 2Me K14, Histone H3 2Me K 14, Histone H3 Me2 Lys14, Histone H3 Me2 Lysine 14, Histone H3 Me2 Lys 14, Histone H3 Me2 K14, Histone H3 Me2 K 14, antibody, antibodies, H3K14Me2, sample

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Validation Data Gallery

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  Histone H3 dimethyl Lys14 pAb tested by Western blot. Detection of dimethylated Histone H3 by Western blot. The analysis was performed using 20 ug HeLa acid extract (10 ug, lane 1) and Histone H3 dimethyl Lys14 pAb at a 1:500 dilution.

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  Histone H3 dimethyl Lys14 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Lys14 antibody for histone H3 dimethylated at Lys14. Methylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated in the left lane next to each row. Top panel: Lane 1: Unmodified lysine 4. Lane 2: Monomethyl lysine 4. Lane 3: Dimethyl lysine 4. Lane 4: Trimethyl lysine 4. Lane 5: Monomethyl lysine 4 #2. Lane 6: Unmodified corresponding to amino acids 6-22 of human histone H3. Lane 7: Monomethyl lysine 9. Lane 8: Dimethyl lysine 9. Lane 9: Trimethyl lysine 9. Bottom panel: Lane 1: Unmodified lysine 27. Lane 2: Monomethyl lysine 27. Lane 3: Dimethyl lysine 27. Lane 4: Trimethyl lysine 27. Lane 5: Monomethyl lysine 14 #2. Lane 6: Dimethyl lysine 14. No detection of peptides (mono-, di-, or trimethylated) corresponding to lysine 56 of histone H3 was observed (data not shown).

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Product Information

• Tested Applications: WB

  Applications Validated by Active Motif: WB: 1:500 - 1:2,000 dilution

• Tested Reactivity: Human, Wide Range Predicted
• Host / Isotype: Rabbit / Serum
• Class: Polyclonal
• Type: Antibody
• Modification: Methylated
• Immunogen: This Histone H3 dimethyl Lys14 antibody was raised against a peptide containing dimethyl-lysine 14 of human histone H3.
• Full Name: Histone H3K14me2 antibody (pAb)
• Synonyms: histone H3, histone, H3, histone-H3, histoneH3, pAb, polyclonal, H3K14me2, Histone H3 dimethyl Lys14, Histone H3 dimethyl Lysine 14, Histone H3 dimethyl Lys 14, Histone H3 dimethyl K14, Histone H3 dimethyl K 14, Histone H3 di-methyl Lys14, Histone H3 di-methyl Lysine 14, Histone H3 di-methyl Lys 14, Histone H3 di-methyl K14, Histone H3 di-methyl K 14, Histone H3 2Me Lys14, Histone H3 2Me Lysine 14, Histone H3 2Me Lys 14, Histone H3 2Me K14, Histone H3 2Me K 14, Histone H3 Me2 Lys14, Histone H3 Me2 Lysine 14, Histone H3 Me2 Lys 14, Histone H3 Me2 K14, Histone H3 Me2 K 14, antibody, antibodies, H3K14Me2, sample
• Molecular weight: 17 kDa
• GenBank accession number: NP_003522
• RRID: AB_2793225
• Purification Method: None
• Buffer: Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue.