Histone H3K27me3 antibody (mAb) (Clone MABI 0323)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Wide Range Predicted

Applications

ChIP, ChIP-Seq, DB, ICC, IF, WB

CloneNo.

MABI 0323

Cat No : 61017,61018

Synonyms

histone H3, histone, H3, histone-H3, histoneH3, mAb, monoclonal, H3K27me3, Histone H3 trimethyl Lys27, Histone H3 trimethyl Lysine 27, Histone H3 trimethyl Lys 27, Histone H3 trimethyl K27, Histone H3 trimethyl K 27, Histone H3 tri-methyl Lys27, Histone H3 tri-methyl Lysine 27, Histone H3 tri-methyl Lys 27, Histone H3 tri-methyl K27, Histone H3 tri-methyl K 27, Histone H3 3Me Lys27, Histone H3 3Me Lysine 27, Histone H3 3Me Lys 27, Histone H3 3Me K27, Histone H3 3Me K 27, Histone H3 Me3 Lys27, Histone H3 Me3 Lysine 27, Histone H3 Me3 Lys 27, Histone H3 Me3 K27, Histone H3 Me3 K 27, antibody, antibodies, chip, chromatin immunoprecipitation, H3K27Me3, polycomb, stem, Clone MABI 0323, stem cell, stem cells, 61319

---

Validation Data Gallery

• 

  Western blot of Histone H3 trimethyl Lys27 antibody. HeLa nuclear extract (20 ug per lane) probed with Histone H3 trimethyl Lys27 antibody (2 ug/ml dilution).

• 

  Dot blot of Histone H3 trimethyl Lys27 antibody. Dot blot analysis was used to confirm the specificity of Histone H3 trimethyl Lys27 antibody for trimethyl Lys27 of histone H3. Recombinant methylated histone proteins corresponding to the immunogen and related sequences were spotted onto PVDF and probed with Histone H3 trimethyl Lys27 at 2 ug/ml. The amount of protein (picomoles) spotted is indicated next to each row. Lane 1: unmodified H3 protein. Lane 2: monomethyl Lys4 protein. Lane 3: dimethyl Lys4 protein. Lane 4: trimethyl Lys4 protein. Lane 5: monomethyl Lys9 protein. Lane 6: dimethyl Lys9 protein. Lane 7: trimethyl Lys9 protein. Lane 8: monomethyl Lys27 protein. Lane 9: dimethyl Lys27 protein. Lane 10: trimethyl Lys27 protein.

---

Product Information

• Tested Applications: ChIP, ChIP-Seq, DB, ICC, IF, WB

  Applications Validated by Active Motif: WB: 0.5 - 2 ug/ml dilution DB: 0.5 - 2 ug/ml dilution CUT&Tag: 1 ug per 50 ul reaction

• Tested Reactivity: Human, Mouse, Wide Range Predicted
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Modification: Methylated
• Immunogen: This Histone H3 trimethyl Lys27 antibody was raised against a peptide containing trimethyl Lys27 of human Histone H3.
• Full Name: Histone H3K27me3 antibody (mAb) (Clone MABI 0323)
• Synonyms: histone H3, histone, H3, histone-H3, histoneH3, mAb, monoclonal, H3K27me3, Histone H3 trimethyl Lys27, Histone H3 trimethyl Lysine 27, Histone H3 trimethyl Lys 27, Histone H3 trimethyl K27, Histone H3 trimethyl K 27, Histone H3 tri-methyl Lys27, Histone H3 tri-methyl Lysine 27, Histone H3 tri-methyl Lys 27, Histone H3 tri-methyl K27, Histone H3 tri-methyl K 27, Histone H3 3Me Lys27, Histone H3 3Me Lysine 27, Histone H3 3Me Lys 27, Histone H3 3Me K27, Histone H3 3Me K 27, Histone H3 Me3 Lys27, Histone H3 Me3 Lysine 27, Histone H3 Me3 Lys 27, Histone H3 Me3 K27, Histone H3 Me3 K 27, antibody, antibodies, chip, chromatin immunoprecipitation, H3K27Me3, polycomb, stem, Clone MABI 0323, stem cell, stem cells, 61319
• Molecular weight: 17 kDa
• GenBank accession number: NP_003522
• RRID: AB_2614987
• Purification Method: Protein G Chromatography
• Buffer: PBS pH 7.5 containing 30% glycerol, 0.3 M NaCl, and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.