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Histone H3K36me3 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Wide Range Predicted

Applications

WB, ChIP, Dot Blot, ChIP-Seq, CUT&Tag

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Cat No : 61101,61102,61902 61101

Synonyms

histone H3, histone, H3, histone-H3, histoneH3, mAb, polyclonal, H3K36me3, Histone H3 trimethyl Lys36, Histone H3 trimethyl Lysine 36, Histone H3 trimethyl Lys 36, Histone H3 trimethyl K36, Histone H3 trimethyl K 36, Histone H3 tri-methyl Lys36, Histone H3 tri-methyl Lysine 36, Histone H3 tri-methyl Lys 36, Histone H3 tri-methyl K36, Histone H3 tri-methyl K 36, Histone H3 3Me Lys36, Histone H3 3Me Lysine 36, Histone H3 3Me Lys 36, Histone H3 3Me K36, Histone H3 3Me K 36, Histone H3 Me3 Lys36, Histone H3 Me3 Lysine 36, Histone H3 Me3 Lys 36, Histone H3 Me3 K36, Histone H3 Me3 K 36, antibody, antibodies, H3K36Me3, chip, sample, chiphsabs

Validation Data Gallery

  • Histone H3K36me3 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from a human glioma cell line. ChIP DNA was sequenced on the Illumina NextSeq and 16.8 million sequence tags were mapped to identify H3K36me3 binding on chromosome 7. The antibody was used at 4 ug per ChIP.

    Histone H3K36me3 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from a human glioma cell line. ChIP DNA was sequenced on the Illumina NextSeq and 16.8 million sequence tags were mapped to identify H3K36me3 binding on chromosome 7. The antibody was used at 4 ug per ChIP.

  • Chromatin IP: ChIP performed using HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) and 10 ug of Histone H3 trimethyl Lys36 antibody (pAb) or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for either the PABPC1 (+ 0.5) or the PABPC1 ( +18) gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

    Chromatin IP: ChIP performed using HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) and 10 ug of Histone H3 trimethyl Lys36 antibody (pAb) or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for either the PABPC1 (+ 0.5) or the PABPC1 ( +18) gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

  • Western blot of Histone H3K36me3 pAb. Nuclear extract of HeLa cells (20 ug) probed with Histone H3K36me3 antibody at a dilution of 0.1 ug/ml.

    Western blot of Histone H3K36me3 pAb. Nuclear extract of HeLa cells (20 ug) probed with Histone H3K36me3 antibody at a dilution of 0.1 ug/ml.

  • Dot blot specificity analysis of Histone H3K26me3 pAb: Dot blot analysis was used to confirm the specificity of Histone H3K36me3 antibody for trimethyl-lysine 36 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto PVDF and probed with the antibody at a dilution of 1 ug/ml. The amount of peptide (in picomoles) spotted is indicated next to each row. Top panel - Lane 1: unmodified Lys4. Lane 2: K4me1. Lane 3: K4me1. Lane 4: K4me2. Lane 5: K4me3. Lane 6: unmodified Lys9. Lane 7: K9me1. Lane 8: K9me2. Lane 9: K9me3. Lower panel - Lane 1: unmodified Lys27. Lane 2: K27me1. Lane 3: K27me2. Lane 4: K27me3. Lane 5: unmodified Lys36. Lane 6: K36me1. Lane 7: K36me2. Lane 8: K36me3.

    Dot blot specificity analysis of Histone H3K26me3 pAb: Dot blot analysis was used to confirm the specificity of Histone H3K36me3 antibody for trimethyl-lysine 36 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto PVDF and probed with the antibody at a dilution of 1 ug/ml. The amount of peptide (in picomoles) spotted is indicated next to each row. Top panel - Lane 1: unmodified Lys4. Lane 2: K4me1. Lane 3: K4me1. Lane 4: K4me2. Lane 5: K4me3. Lane 6: unmodified Lys9. Lane 7: K9me1. Lane 8: K9me2. Lane 9: K9me3. Lower panel - Lane 1: unmodified Lys27. Lane 2: K27me1. Lane 3: K27me2. Lane 4: K27me3. Lane 5: unmodified Lys36. Lane 6: K36me1. Lane 7: K36me2. Lane 8: K36me3.

Product Information

Tested Applications WB, ChIP, Dot Blot, ChIP-Seq, CUT&Tag

Applications Validated by Active Motif: ChIP: 10 ug per ChIP ChIP-Seq: 4 ug each WB: 0.5 - 2 ug/ml dilution CUT&Tag: 1 ug per 50 ul reaction For Histone H3K36me3, we also offer AbFlex Histone H3K36me3 Recombinant Antibody (rAb). For details, see Catalog No. 91265.
Tested Reactivity Human, Mouse, Wide Range Predicted
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
ModificationMethylated
Immunogen This Histone H3 trimethyl Lys36 antibody was raised against a peptide containing trimethyl Lys36 of human histone H3.
Full Name Histone H3K36me3 antibody (pAb)
Synonyms histone H3, histone, H3, histone-H3, histoneH3, mAb, polyclonal, H3K36me3, Histone H3 trimethyl Lys36, Histone H3 trimethyl Lysine 36, Histone H3 trimethyl Lys 36, Histone H3 trimethyl K36, Histone H3 trimethyl K 36, Histone H3 tri-methyl Lys36, Histone H3 tri-methyl Lysine 36, Histone H3 tri-methyl Lys 36, Histone H3 tri-methyl K36, Histone H3 tri-methyl K 36, Histone H3 3Me Lys36, Histone H3 3Me Lysine 36, Histone H3 3Me Lys 36, Histone H3 3Me K36, Histone H3 3Me K 36, Histone H3 Me3 Lys36, Histone H3 Me3 Lysine 36, Histone H3 Me3 Lys 36, Histone H3 Me3 K36, Histone H3 Me3 K 36, antibody, antibodies, H3K36Me3, chip, sample, chiphsabs
Molecular weight 17 kDa
GenBank accession numberNP_003522
RRIDAB_2615073
Purification Method Protein A Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.