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HuR Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11910-1-AP
ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG
|Positive WB detected in||HEK-293 cells, A431 cells, HeLa cells, hESC cells, HL-60 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, RAW264.7|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human ovary tumor tissue, human breast cancer tissue, human pancreas tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, Ethacrynic acid treated HepG2 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11910-1-AP targets HuR in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HuR fusion protein Ag2249|
|Full Name||ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)|
|Calculated molecular weight||326aa,36 kDa|
|Observed molecular weight||30-37 kDa|
|GenBank accession number||BC003376|
|Gene ID (NCBI)||1994|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
|Product Specific Protocols|
|WB protocol for HuR antibody 11910-1-AP||Download protocol|
|IHC protocol for HuR antibody 11910-1-AP||Download protocol|
|IF protocol for HuR antibody 11910-1-AP||Download protocol|
|IP protocol for HuR antibody 11910-1-AP||Download protocol|
|FC protocol for HuR antibody 11910-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.
Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.
Protease 2A induces stress granule formation during coxsackievirus B3 and enterovirus 71 infections.
Int J Mol Sci
HuR and TIA1/TIAL1 Are Involved in Regulation of Alternative Splicing of SIRT1 Pre-mRNA.
Int J Mol Sci
Post-Transcriptional Up-Regulation of PDGF-C by HuR in Advanced and Stressed Breast Cancer.
DNA Cell Biol
Dcp1a and GW182 Induce Distinct Cellular Aggregates and Have Different Effects on microRNA Pathway.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
S (Verified Customer) (05-18-2020)
CHAO (Verified Customer) (01-30-2020)
Weak band for the heart lysates
Gaston (Verified Customer) (05-16-2019)
Antibody worked very well for wester blot detection and immunofluorescence in Huh7 and SH-SY5Y cells. Did not observe any signs of deteriorating detection when stored at -20 for over two years.