• Featured Product
  • KD/KO Validated

HuR Polyclonal antibody

HuR Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11910-1-AP

Synonyms

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, HeLa cells, hESC cells, HL-60 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, RAW264.7
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human pancreas tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, Ethacrynic acid treated HepG2 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11910-1-AP targets HuR in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HuR fusion protein Ag2249
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Calculated molecular weight 326 aa, 36 kDa
Observed molecular weight 30-37 kDa
GenBank accession numberBC003376
Gene symbol ELAVL1
Gene ID (NCBI) 1994
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

Protocols

Product Specific Protocols
WB protocol for HuR antibody 11910-1-APDownload protocol
IHC protocol for HuR antibody 11910-1-APDownload protocol
IF protocol for HuR antibody 11910-1-APDownload protocol
IP protocol for HuR antibody 11910-1-APDownload protocol
FC protocol for HuR antibody 11910-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.

Authors - Erika C Urdaneta
humanWB

RNA Biol

Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.

Authors - Sikandar Azam
humanIF

Virol J

Protease 2A induces stress granule formation during coxsackievirus B3 and enterovirus 71 infections.

Authors - Shuo Wu
humanWB

Pregnancy Hypertens

LncRNA MALAT1 affects the migration and invasion of trophoblast cells by regulating FOS expression in early-onset preeclampsia.

Authors - Qin Li
  • KD Validated
humanWB

Int J Mol Sci

HuR and TIA1/TIAL1 Are Involved in Regulation of Alternative Splicing of SIRT1 Pre-mRNA.

Authors - Wenhui Zhao
humanWB, IHC

Int J Mol Sci

Post-Transcriptional Up-Regulation of PDGF-C by HuR in Advanced and Stressed Breast Cancer.

Authors - Nian-An Luo
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

S (Verified Customer) (05-18-2020)

Good Antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: AC16
HuR Antibody Western Blot validation (1:1000 dilution) in AC16 (Cat no:11910-1-AP)
FH

CHAO (Verified Customer) (01-30-2020)

Weak band for the heart lysates

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart
FH

Gaston (Verified Customer) (05-16-2019)

Antibody worked very well for wester blot detection and immunofluorescence in Huh7 and SH-SY5Y cells. Did not observe any signs of deteriorating detection when stored at -20 for over two years.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:250; 1:6500
  • Cell Tissue Type: Huh7, SH-SY5Y, Vero
HuR Antibody Western Blot,Immunofluorescence, validation (1:250; 1:6500 dilution) in Huh7, SH-SY5Y, Vero (Cat no:11910-1-AP)