|Positive WB detected in||HEK-293 cells, A431 cells, HeLa cells, hESC cells, HL-60 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, RAW264.7|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human ovary tumor tissue, human breast cancer tissue, human pancreas tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, Ethacrynic acid treated HepG2 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11910-1-AP targets HuR in WB, RIP, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HuR fusion protein Ag2249|
|Full Name||ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)|
|Calculated molecular weight||326 aa, 36 kDa|
|Observed molecular weight||30-37 kDa|
|GenBank accession number||BC003376|
|Gene ID (NCBI)||1994|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
|Product Specific Protocols|
|WB protocol for HuR antibody 11910-1-AP||Download protocol|
|IHC protocol for HuR antibody 11910-1-AP||Download protocol|
|IF protocol for HuR antibody 11910-1-AP||Download protocol|
|IP protocol for HuR antibody 11910-1-AP||Download protocol|
|FC protocol for HuR antibody 11910-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.
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Protease 2A induces stress granule formation during coxsackievirus B3 and enterovirus 71 infections.
LncRNA MALAT1 affects the migration and invasion of trophoblast cells by regulating FOS expression in early-onset preeclampsia.
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Post-Transcriptional Up-Regulation of PDGF-C by HuR in Advanced and Stressed Breast Cancer.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
S (Verified Customer) (05-18-2020)
CHAO (Verified Customer) (01-30-2020)
Weak band for the heart lysates
Gaston (Verified Customer) (05-16-2019)
Antibody worked very well for wester blot detection and immunofluorescence in Huh7 and SH-SY5Y cells. Did not observe any signs of deteriorating detection when stored at -20 for over two years.