Human ABCD1 ELISA Kit
Cat no : KE00030
Synonyms
ABC42, ABCD1, Adrenoleukodystrophy protein, ALD, ALDP, AMN
Validation Data Gallery
Product Information
KE00030 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ABCD1 ELISA kit is to be used to detect and quantify protein levels of endogenous ABCD1. The assay recognizes human ABCD1. An antibody specific for ABCD1 has been pre-coated onto the microwells. The ABCD1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for ABCD1 is added to detect the captured ABCD1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ABCD1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, cell lysates |
| Assay type | Sandwich |
| Sensitivity | 6.0 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 215 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 99% | 79%-123% |
| Human plasma | 93% | 85%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,689.5 | 138.9 | 8.2 |
| 2 | 20 | 1,243.4 | 117.6 | 9.5 |
| 3 | 20 | 104.7 | 3.6 | 3.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,599.1 | 122.2 | 7.6 |
| 2 | 24 | 836.0 | 50.7 | 6.1 |
| 3 | 24 | 105.2 | 4.2 | 4.0 |
Background Information
ABCD1, also known as adrenoleukodystrophy protein (ALDP), is a member of the ATP-binding cassette (ABC) transporter superfamily. ALDP is a multi-pass membrane protein and localized at peroxisome membrane. Various mutations of ABCD1 gene cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex. It results in mental deterioration, corticospinal tract dysfunction, and cortical blindness. ALDP is supposed to function as a homodimer allowing the entry of CoA-esters of very-long chain fatty acids into the peroxisome, the unique site of their β-oxidation. However, the defined function of ALDP has not been well investigated so far.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ABC42, ABCD1, Adrenoleukodystrophy protein, ALD, ALDP, AMN |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.