Human APCS ELISA Kit

Sensitivity

2.6 pg/mL

Range

15.6 - 1000 pg/mL

Reactivity

Human

Cat no : KE00122

Synonyms

9.5S alpha 1 glycoprotein, amyloid P component, serum, APCS, PTX2, SAP, Serum amyloid P component



Product Information

KE00122 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APCS ELISA kit is to be used to detect and quantify protein levels of endogenous APCS. The assay recognizes human APCS. An antibody specific for APCS has been pre-coated onto the microwells. The APCS protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for APCS is added to detect the captured APCS protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human APCS ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, plasma, Cell culture supernatants, Urine
Assay type Sandwich
Sensitivity 2.6 pg/mL
Range 15.6 - 1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plasma109%90%-121%
Human urine103%75%-119%
Cell culture supernatants94%81%-126%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12024.02.610.9
220100.47.17.1
320432.718.24.2

InterAssay

Samplenmean ( pg/mL)SDCV%
12428.92.99.9
224106.210.19.5
324457.745.09.8

Background Information

Serum amyloid P component (SAP, also known as APCS), belongs to the family of pentraxins, a superfamily of plasma proteins characterized by their pentameric assembly and calcium-dependent ligand binding. SAP is a constitutive serum protein synthesized by hepatocytes. It is structurally related to C-reactive protein, a major acute-phase reactant in humans. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. SAP can also bind to fibrils in all types of amyloid deposits, and contributes to the pathogenesis of amyloidosis.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms 9.5S alpha 1 glycoprotein, amyloid P component, serum, APCS, PTX2, SAP, Serum amyloid P component