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Human APCS ELISA Kit0 Publications

Catalog number: KE00122

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Freight/Packing £00.00

Size: 09µ/gram

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Standard curve of KE00122 Human APCS ELISA Kit

Standard curve of KE00122 Human APCS ELISA Kit


Product name:

1 X 96 well plate

Sample type:
Serum, plasma, Cell culture supernatants, Urine

Assay type:

2.6 pg/mL

15.6 - 1000 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Human plasma 109% 90%-121%
Human urine 103% 75%-119%
Cell culture supernatants 94% 81%-126%

Samplenmean ( pg/mL)SDCV%
1 20 24.0 2.6 10.9
2 20 100.4 7.1 7.1
3 20 432.7 18.2 4.2

Samplenmean ( pg/mL)SDCV%
1 24 28.9 2.9 9.9
2 24 106.2 10.1 9.5
3 24 457.7 45.0 9.8

Product overview:
KE00122 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APCS ELISA kit is to be used to detect and quantify protein levels of endogenous APCS. The assay recognizes human APCS. An antibody specific for APCS has been pre-coated onto the microwells. The APCS protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for APCS is added to detect the captured APCS protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

9.5S alpha 1 glycoprotein, amyloid P component, serum, APCS, PTX2, SAP, Serum amyloid P component

Serum amyloid P component (SAP, also known as APCS), belongs to the family of pentraxins, a superfamily of plasma proteins characterized by their pentameric assembly and calcium-dependent ligand binding. SAP is a constitutive serum protein synthesized by hepatocytes. It is structurally related to C-reactive protein, a major acute-phase reactant in humans. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. SAP can also bind to fibrils in all types of amyloid deposits, and contributes to the pathogenesis of amyloidosis.

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