Human B7-H3/CD276 ELISA Kit
Cat no : KE00143
Synonyms
CD276, 4Ig-B7-H3, B7 H3, B7 homolog 3, B7H3
Validation Data Gallery
Product Information
KE00143 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The B7-H3/CD276 ELISA kit is to be used to detect and quantify protein levels of endogenous B7-H3/CD276. The assay recognizes human B7-H3/CD276. An antibody specific for B7-H3/CD276 has been pre-coated onto the microwells. The B7-H3/CD276 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for B7-H3/CD276 is added to detect the captured B7-H3/CD276 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human B7-H3/CD276 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Cell lysates, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.6 pg/mL |
| Range | 9.4-600 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 80381 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 85% | 77%-93% |
| urine | 87% | 79%-97% |
| Cell culture supernatants | 88% | 76%-104% |
| cell lysates | 88% | 75%-101% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 289.4 | 8.5 | 2.9 |
| 2 | 20 | 60.4 | 1.3 | 2.1 |
| 3 | 20 | 14.2 | 0.4 | 2.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 289.0 | 10.3 | 3.6 |
| 2 | 24 | 60.1 | 1.7 | 2.9 |
| 3 | 24 | 14.3 | 0.6 | 4.4 |
Background Information
B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor. Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy. Soluble form of B7-H3 (sB7-H3), released by monocytes, dendritic cells, activated T cells, and B7-H3-positive tumor cells, has been detected in human blood samples and cell culture supernatants.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD276, 4Ig-B7-H3, B7 H3, B7 homolog 3, B7H3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
J Clin Lab Anal Evaluation of serum B7-H3 expression, ultrasound and clinical characteristics to predict the risk of cervical lymph node metastases in papillary thyroid carcinoma by nomogram | ||
Front Cell Dev Biol Enhanced T-cell activation and chemokine-associated function in CD14-positive cells from venous sinus blood in sub-acute cerebral venous sinus thrombosis |