Product name:

Human/Rat BDNF ELISA Kit

Tests:

1 X 96 well plate

Sample type:

Serum, Plasma, Cell culture supernatants

Assay type:

Sandwich

Sensitivity:

0.1 pg/mL

Range:

12.5 - 800 pg/mL

Reacted Species:

Human/Rat

Tested applications:

Sandwich ELISA

Recovery:

Sample Type	Average	Range
Human plasma	92%	74%-117%
Cell culture supernatants	90%	73%-113%

Product overview:

KE00096 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The BDNF ELISA kit is to be used to detect and quantify protein levels of endogenous BDNF. The assay recognizes human BDNF. A polyclonal antibody specific for BDNF has been pre-coated onto the microwells. The BDNF protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for human BDNF is added to detect the captured human BDNF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Storage Instructions:

All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:

Abrineurin, BDNF