Human/Rat BDNF ELISA Kit

Sensitivity

0.1 pg/mL

Range

12.5 - 800 pg/mL

Reacted Species

Human/Rat

Cat no : KE00096

Synonyms

Abrineurin, BDNF



Product Information

KE00096 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The BDNF ELISA kit is to be used to detect and quantify protein levels of endogenous BDNF. The assay recognizes human BDNF. An antibody specific for BDNF has been pre-coated onto the microwells. The BDNF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human BDNF is added to detect the captured human BDNF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human/Rat BDNF ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.1 pg/mL
Range 12.5 - 800 pg/mL
Reacted Species Human/Rat
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plasma92%74%-117%
Cell culture supernatants90%73%-113%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12035.82.36.3
220116.59.27.9
320513.936.97.2

InterAssay

Samplenmean ( pg/mL)SDCV%
12432.22.68.1
224108.68.27.6
324517.340.87.9

Background Information

The brain-derived neurotrophic factor (BDNF), as a member of the neurotrophin family of growth factors, is broadly expressed in the central nervous system. BDNF is involved in cerebral morphogenesis, synaptic plasticity and learning, mental health, degenerative central nervous system (CNS) diseases, and energy metabolism. BDNF has recently been identified as one of the four major pharmaceutical targets in neuropsychiatric diseases, including depression and bipolar disorder. In addition, BDNF is increasingly considered as a potential therapeutic agent for neurodevelopmental disorders such as Rett syndrome.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms Abrineurin, BDNF

Publications

SpeciesSample TypeTitle
humanplasma

Med Sci Monit

Protective Effects of Dexmedetomidine and Oxycodone in Patients Undergoing Limb Ischemia-Reperfusion.

Authors - Wenjie Cheng
humanserum

Evid Based Complement Alternat Med

Shenmayizhi Formula Combined with Ginkgo Extract Tablets for the Treatment of Vascular Dementia: A Randomized, Double-Blind, Controlled Trial.

Authors - Huiqin Zhang
humanplasma

Dose Response

Intraoperative Dexmedetomidine in Peripheral or Emergency Neurologic Surgeries of Patients With Mild-to-Moderate Traumatic Brain Injuries: A Retrospective Cohort Study.

Authors - Qin Ding

Int J Mol Sci

Elucidation of the Molecular Mechanism Underlying Lippia citriodora(Lim.)-Induced Relaxation and Anti-Depression.

Authors - Mouad Sabti
mousetissue homogenates

Int J Mol Sci

Elucidation of the Molecular Mechanism Underlying Lippia citriodora(Lim.)-Induced Relaxation and Anti-Depression.

Authors - Mouad Sabti