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Human/Rat BDNF ELISA Kit3 Publications

Catalog number: KE00096

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$399.00

Freight/Packing £00.00

Size: 09µ/gram

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Standard curve of KE00096 Human/Rat BDNF ELISA Kit

Overview

Product name:
Human/Rat BDNF ELISA Kit
Tests:
1 X 96 well plate
Sample type:
Serum, Plasma, Cell culture supernatants
Assay type:
Sandwich
Sensitivity:
0.1 pg/mL
Range:
12.5 - 800 pg/mL
Reacted Species:
Human/Rat
Tested applications:
Sandwich ELISA
Recovery:
Sample TypeAverageRange
Human plasma 92% 74%-117%
Cell culture supernatants 90% 73%-113%
IntraAssay:
Samplenmean ( pg/mL)SDCV%
1 20 35.8 2.3 6.3
2 20 116.5 9.2 7.9
3 20 513.9 36.9 7.2
InterAssay:
Samplenmean ( pg/mL)SDCV%
1 24 32.2 2.6 8.1
2 24 108.6 8.2 7.6
3 24 517.3 40.8 7.9
Product overview:
KE00096 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The BDNF ELISA kit is to be used to detect and quantify protein levels of endogenous BDNF. The assay recognizes human BDNF. An antibody specific for BDNF has been pre-coated onto the microwells. The BDNF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human BDNF is added to detect the captured human BDNF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Properties

Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms:
Abrineurin, BDNF
Background

The brain-derived neurotrophic factor (BDNF), as a member of the neurotrophin family of growth factors, is broadly expressed in the central nervous system. BDNF is involved in cerebral morphogenesis, synaptic plasticity and learning, mental health, degenerative central nervous system (CNS) diseases, and energy metabolism. BDNF has recently been identified as one of the four major pharmaceutical targets in neuropsychiatric diseases, including depression and bipolar disorder. In addition, BDNF is increasingly considered as a potential therapeutic agent for neurodevelopmental disorders such as Rett syndrome.


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