Human CD146/MCAM ELISA Kit0 Publications

Catalog number: KE00065

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Size: 09µ/gram

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Product name:
Human CD146/MCAM ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatants

Assay type:

3.8 ng/mL

62.5-4000 ng/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatants 95% 80%-125%
Human plasma 92% 79%-121%

Samplenmean ( ng/mL)SDCV%
1 20 2,643.0 252.8 9.6
2 20 487.4 40.3 8.3
3 20 124.3 7.3 5.9

Samplenmean ( ng/mL)SDCV%
1 24 1,647.6 156.9 9.5
2 24 344.9 27.6 8.0
3 24 82.4 8.1 9.8

Product overview:
KE00065 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MCAM ELISA kit is to be used to detect and quantify protein levels of endogenous MCAM. The assay recognizes human MCAM. An antibody specific for MCAM has been pre-coated onto the microwells. The MCAM protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for MCAM is added to detect the captured MCAM protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

CD146, MCAM, MUC18

MCAM, also known as melanoma cell adhesion molecule (CD146) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. A soluble form of CD146 has also been reported. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs).

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