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Recombinant Human CD28 protein (hFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

hFc Tag

Activity

EC50: 6-24 ng/mL

Cat no : Eg0969

Validation Data Gallery

  • Purity of Recombinant Human CD28 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD28 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human CD80 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human CD28 (hFc tag) with a linear range of 6-24 ng/mL.

    Immobilized Human CD80 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human CD28 (hFc tag) with a linear range of 6-24 ng/mL.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human CD80 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human CD28 (hFc tag) with a linear range of 6-24 ng/mL.
Expression HEK293-derived Human CD28 protein Asn19-Pro152 (Accession# P10747-1) with a human IgG1 Fc tag at the C-terminus.
GeneID 940
Accession P10747-1
PredictedSize 41.1 kDa
SDS-PAGE 52-65 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Cluster of Differentiation 28 (CD28, also known as Tp44) is one of the proteins constitutively expressed on the surface of 80% of human T cells. CD28 is a highly glycosylated membrane protein, formed by two homodimer disulfide-linked peptides, which shares homology with the immunoglobulin superfamily. CD28 signals play a key role in many T cell processes including cytoskeletal remodeling, production of cytokines, survival, and differentiation. CD28 interactions with the B7 family of costimulatory ligands are essential for initiating antigen-specific T cell responses, upregulating cytokine expression and promoting T cell expansion and differentiation.

References:

1.Hansen JA. et al. (1980).  Immunogenetics. 10(1-4): 247-260.
2.Aruffo A. et al. (1987). Proc Natl Acad Sci U S A. 84(23): 8573-8577.
3.Jonathan H Esensten. et al. (2016). Immunity. 44(5):973-988.
4.D J Lenschow. et al. (1996). Annu Rev Immunol. 14:233-258.