Product name:

Human CD3E Kit

Tests:

1 X 96 well plate

Sample type:

serum; Plasma

Assay type:

Sandwich

Sensitivity:

5 pg/mL

Range:

31.25-2000 pg/mL

Reacted Species:

Human

Tested applications:

Sandwich ELISA

Recovery:

Sample Type	Average	Range
Plasma	94	82-107

Product overview:

KE00026 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD3E ELISA kit is to be used to detect and quantify protein levels of endogenous CD3E. The assay recognizes human CD3E. A polyclonal antibody specific for CD3E has been pre-coated onto the microwells. The CD3E protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for CD3E is added to detect the captured CD3E protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Storage Instructions:

All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:

CD3E, T3E, TCRE