Human CHGA ELISA Kit
Cat no : KE00057
Synonyms
CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2
Validation Data Gallery
Product Information
KE00057 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CHGA ELISA kit is to be used to detect and quantify protein levels of endogenous CHGA. The assay recognizes human CHGA. An antibody specific for CHGA has been pre-coated onto the microwells. The CHGA protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for CHGA is added to detect the captured CHGA protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CHGA ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 30.0 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1113 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 96% | 83%-114% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4,347.9 | 373.3 | 8.6 |
| 2 | 20 | 1,515.1 | 63.7 | 4.3 |
| 3 | 20 | 338.5 | 9.5 | 2.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 5,060.1 | 474.0 | 9.4 |
| 2 | 24 | 1,533.1 | 116.1 | 7.6 |
| 3 | 24 | 330.0 | 15.9 | 4.8 |
Background Information
Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors. However, CgA is not a tumor-specific antigen for NETs, and an abnormal concentration has been described in some non-malignant diseases such as renal failure, heart failure, proton pump inhibition, gastritis, hypertension, or patients with liver diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Ann Surg MiR-7-5p as a Diagnostic and Monitoring Biomarker for Neuroendocrine Tumors: Insights from the SKY-NET Multicenter Study |