Human CXCL16 ELISA Kit1 Publications

Catalog number: KE00052

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$399.00

Freight/Packing £00.00

Size: 09µ/gram

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Overview


Product name:
Human CXCL16 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatants

Assay type:
Sandwich

Sensitivity:
0.1 pg/mL

Range:
7.8-500 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Human serum 91% 76%-103%
Human plasma 99% 83%-119%
Cell culture supernatants 87% 82%-118%

IntraAssay:
Samplenmean ( pg/mL)SDCV%
1 20 241.1 10.8 4.5
2 20 56.1 1.7 3.0
3 20 12.7 0.5 3.9

InterAssay:
Samplenmean ( pg/mL)SDCV%
1 24 260.7 17.1 6.6
2 24 56.0 3.2 5.8
3 24 12.7 0.7 5.8

Product overview:
KE00052 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CXCL16 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL16. The assay recognizes human CXCL16. An antibody specific for CXCL16 has been pre-coated onto the microwells. The CXCL16 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for CXCL16 is added to detect the captured CXCL16 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
C X C motif chemokine 16, CXCL16, CXCLG16, SCYB16, Small inducible cytokine B16, SR PSOX, SRPSOX, Transmembrane chemokine CXCL16
Background

CXC chemokine ligand 16 (CXCL16) is a CXC soluble chemokine, an adhesion molecule and a cell surface scavenger receptor. CXCL16 regulates inflammation, tissue injury and fibrosis. CXCL16 exists as a transmembrane form (TM-CXCL16) as well as a soluble form (sCXCL16) that is cleaved by proteolytic enzymes. CXCL16 is produced by several inflammatory cells including macrophages, dendritic cells, smooth muscle cells or lymphocytes. CXCL16 is expressed in various cancers, including pancreatic, prostate, breast, colorectal, and nonsmall cell lung cancer. CXCL16) has been proposed as an important pathogenic mediator in inflammatory diseases, such as rheumatoid arthritis, hepatitis or atherosclerosis.


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