Human CXCL16 ELISA Kit1 Publications

Catalog number: KE00052

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Freight/Packing £00.00

Size: 09µ/gram

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Product name:
Human CXCL16 ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatants

Assay type:

0.1 pg/mL

7.8-500 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Human serum 91% 76%-103%
Human plasma 99% 83%-119%
Cell culture supernatants 87% 82%-118%

Samplenmean ( pg/mL)SDCV%
1 20 241.1 10.8 4.5
2 20 56.1 1.7 3.0
3 20 12.7 0.5 3.9

Samplenmean ( pg/mL)SDCV%
1 24 260.7 17.1 6.6
2 24 56.0 3.2 5.8
3 24 12.7 0.7 5.8

Product overview:
KE00052 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CXCL16 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL16. The assay recognizes human CXCL16. A polyclonal antibody specific for CXCL16 has been pre-coated onto the microwells. The CXCL16 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for CXCL16 is added to detect the captured CXCL16 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

C X C motif chemokine 16, CXCL16, CXCLG16, SCYB16, Small inducible cytokine B16, SR PSOX, SRPSOX, Transmembrane chemokine CXCL16

CXCL16 is a recently discovered cytokine belonging to the CXC chemokine family, which is synthesised in plasmacytoid dendritic cell as a transmembrane molecule. It exists in a transmembrane and soluble form. The transmembrane form of CXCL16 functions as an adhesion molecule for CXCR6-expressing cells, whereas the soluble form of CXCL16 mediates infiltration of circulating cells into sites of injury. CXCL16, has been proposed as an important pathogenic mediator in inflammatory diseases, including rheumatoid arthritis, glomerulonephritis, or prostate cancer. CXCL16 has been implicated in some forms of renal disease such as lupus nephritis and antiglomerular basement membrane nephritis. CXCL16 also plays a pivotal role in the pathogenesis of angiotensin II–induced renal injury and fibrosis through regulation of macrophage and T cell infiltration and bone marrow–derived fibroblast accumulation.

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