Human Caspase 9 ELISA Kit
Cat no : KE00691
Synonyms
CASP9, Caspase 9/p35/p10, APAF 3, APAF3, APAF-3
Validation Data Gallery
Product Information
KE00691 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Caspase 9 ELISA kit is to be used to detect and quantify protein levels of endogenous human Caspase 9. The assay recognizes human Caspase 9. An antibody specific for human Caspase 9 has been pre-coated onto the microwells. The human Caspase 9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Caspase 9 is added to detect the captured human Caspase 9 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Caspase 9 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.234-15 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 842 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 79% | 77%-82% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 7.32 | 0.43 | 5.94 |
| 2 | 8 | 1.93 | 0.13 | 6.76 |
| 3 | 8 | 0.95 | 0.05 | 4.75 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 7.30 | 0.38 | 5.17 |
| 2 | 16 | 2.02 | 0.12 | 6.06 |
| 3 | 16 | 0.95 | 0.04 | 4.23 |
Background Information
Caspase 9, apoptosis-related cysteine protease (CASP9) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. 10380-1-AP can recognize the pre- and cleaved- caspase 9. In recent years, the localization of caspase 9 was a focus of interest.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CASP9, Caspase 9/p35/p10, APAF 3, APAF3, APAF-3 |