Recombinant Human Cathepsin B protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg1020

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Validation Data Gallery

• 

  Purity of Recombinant Human Cathepsin B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Cathepsin B protein Arg18-Ile339 (Accession# P07858) with a His tag at the C-terminus.
• GeneID: 1508
• Accession: P07858
• PredictedSize: 37.5 kDa
• SDS-PAGE: 37-45kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Cardiovascular diseases persist as a major threat to human health. Cathepsin B (CTSB), a ubiquitous lysosomal cysteine protease, participates in a multitude of physiological processes and plays a pivotal role in the progression of diverse pathological conditions. CTSB, a member of the C1 family, is extensively expressed in various tissues and cell types throughout the body, often referred to as a housekeeping enzyme.

References:

1.Liu F.et al.(2024) Eur J Med Chem.269:116329.
2.Turk V.et al.(2012) Biochim Biophys Acta.1824(1):68-88.
3.Ruiz-Blazquez P.et al.(2021) J Heaton.75(5):1192-1202.
4.Ketterer S.et al.(2017) FEBS J.284(10):1437-1454.
5.Liu CL.et al.(2018) Nat Rev Cardiol.15(6):351-370.